Abstract
Intracerebral hemorrhage (ICH) is a devastating subtype of stroke with high disability/mortality. Baicalein has strong anti-inflammatory activity. This study aims to explore the mechanism of baicalein on brain injury after ICH. The model of brain injury after ICH was established by collagenase induction, followed by the evaluation of neurological severity, brain water content, the degenerated neurons, neuronal apoptosis, and reactive oxygen species (ROS). The ICH model was treated with baicalein or silencing NLRP3 to detect brain injury. The expression of NLRP3 inflammasome was detected after treatment with ROS scavenger. The expressions of oxidative stress markers and inflammatory factors were detected, and the levels of components in NLRP3 inflammasome were detected. Baicalein reduced the damage of nervous system, lesion surface, brain water content, and apoptosis. Baicalein inhibited malondialdehyde and increased IL-10 by inhibiting ROS in brain tissue after ICH. Baicalein inhibited the high expression of NLRP3 inflammasome in ICH. ROS scavenger inhibited the NLRP3 inflammatory response by inhibiting ROS levels. Silencing NLRP3 alleviated the brain injury after ICH by inhibiting excessive oxidative stress and inflammatory factors. Overall, baicalein alleviated the brain injury after ICH by inhibiting ROS and NLRP3 inflammasome.
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XC is the guarantor of integrity of the entire study; XC and YZ contributed to the study concepts, study design, and definition of intellectual content; SSW contributed to the literature research; XC contributed to the manuscript preparation and XC contributed to the manuscript editing and review; SSW and WW contributed to the clinical studies; XC, YZ, SSW, and WW contributed to the experimental studies and data acquisition; XC and YZ contributed to the data analysis and statistical analysis. All the authors read and approved the final manuscript.
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Animal experiments followed the standards established by the animal experiment committee of the Fourth Affiliated Hospital of China Medical University and approved by the ethics committee of the Fourth Affiliated Hospital of China Medical University. All animal experiments were conducted according to the “Guidelines for the Care and Use of Laboratory Animals.”
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Chen, X., Zhou, Y., Wang, S. et al. Mechanism of Baicalein in Brain Injury After Intracerebral Hemorrhage by Inhibiting the ROS/NLRP3 Inflammasome Pathway. Inflammation 45, 590–602 (2022). https://doi.org/10.1007/s10753-021-01569-x
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DOI: https://doi.org/10.1007/s10753-021-01569-x