Abstract
Currently, in vitro cell, tissue, and organ cultures are used to produce plant secondary metabolites that are used as natural coloring agents, nutraceuticals, and medications. Various strategies have been applied for the hyperaccumulation of biomass and bioactive secondary compounds in vitro. The elicitation of cultured cells and organs with biotic and abiotic elicitors is an excellent strategy that has yielded promising results. Among various abiotic elicitors, light parameters such as light quality, intensity, and photoperiod have evolved as biotechnological tools to elicit cultures. Of the various light sources tested, ultraviolet (UV) lights, particularly UV-B, red, blue, and a mixture of light emitted by fluorescent light or light-emitting diodes, have yielded outstanding results and boosted the accumulation of bioactive compounds in cultured cells and organs. The objective of the current study was to evaluate light as an elicitor source and summarize the advantages and limitations of various light sources as elicitors for the bioaccumulation of secondary metabolites in vitro. The mechanism of the elicitation of secondary metabolism by UV and spectral light is discussed in this review.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbasi BH, Khan T, Khurshid R, Nadeem M, Drouet S, Hano C (2021) UV-C mediated accumulation of pharmacologically significant phytochemicals under light regimes in in vitro culture of Fagonia indica (L). Sci Rep 11:679. https://doi.org/10.1038/s41598-020-79896-6
Adil M, Abbasi BH, Haq IU (2019) Red light controlled callus morphogenetic patterns and secondary metabolites production in Withania somnifera L. Biotechnol Rep 24:e00380. https://doi.org/10.1016/j.btre.2019.e00380
Ali H, Khan MA, Ullah N, Khan RS (2018) Impacts of hormonal elicitors and photoperiod regimes on elicitation of bioactive secondary volatiles in cell cultures of Ajuga bracteosa. J Photoch Photobiol B 183:242–250. https://doi.org/10.1016/j.jphotobiol.2018.04.044
Alvarado-Orea IV, Paniagua-Vega D, Capataz-Tafur J, Torres-Lopes A, Vera-Reyes I, Garcia-Lopez E, Huerta-Heredia AA (2020) Photoperiod and elicitors increase steviol glycosides, phenolics, and flavonoid contents in root cultures of Stevia Rabaudiana. Vitro Cell Dev Biol Plant 56:298–306. https://doi.org/10.1007/s11627-019-10041-3
Alvarenga ICA, Pacheco FV, Silva ST, Bertolucci SKV, Pinto JEBP (2015) In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles. Plant Cell Tissue Organ Cult 122:299–308. https://doi.org/10.1007/s11240-015-0766-7
Andrade HB, Braga AF, Bertolucci SKV, HSie BS, Silva ST, Pinto JEBP (2017) Effect of plant growth regulators, light intensity and LED on growth and volatile compound of Hyptis suaveolens (L.) Poit in vitro plantlets. 1155:277–284. https://doi.org/10.17660/ActaHortic.2017.1155.40
Anjum S, Abbasi BH, Doussot J, Favre-Reguillon A, Hano C (2017) Effects of photoperiod regimes and ultraviolet-C radiations on biosynthesis of industrially important lignans and neolignans in cell cultures of Linum usitatissimum L. (Flax). J Phochem Photobiol B 167:216–227. https://doi.org/10.1016/j.jphotobiol.2017.01.006
Bantis F, Smirnakou S, Ouzounis T, Koukounaras A, Ntagkas N, Radoglou K (2018) Current status and recent achievements in the field of horticulture with the use of light-emitting diodes (LEDs). Sci Hrotic 235:437–451. https://doi.org/10.1016/j.scienta.2018.02.058
Batista DS, de Castro KM, Silva ARD, Teixeira ML, Sales TA, Soares LI, Cardoso MDG, Santos MDO, Viccini LF, Otoni WC (2016) Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae). Vitro Cell Dev Biol – Plant 52:276–282. https://doi.org/10.1007/s11627-016-9761-x
Batista DS, Felipe SHS, Silva TD, de Castro KM, Mamedes-Rodrigues TC, Miranda NA, Rios-Rios AM, Faria DV, Fortini EA, Chagas K, Torres-Silva G, Xavier A, Arencibia AD, Otoni WC (2018) Light quality in plant tissue culture: does it matter? Vitro Cell Dev Biol – Plant 54:195–215. https://doi.org/10.1007/s11627-018-9902-5
Beuel AK, Jablonka N, Heesel J, Severin K, Spiegel H, Rasche S (2021) LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures. Sci Rep 11:23353. https://doi.org/10.1038/s41598-021-02762-6
Binder BYK, Peebles CAM, Shanks JV, San KY (2009) The effects of UV-B stress on the production of terpenoid indole alkaloids in Catharanthus roseus hairy roots. Biotechnol Prog 25:861–865. https://doi.org/10.1021/bp.97
Biswal B, Jen B, Giri AK, Acharya L (2022) Monochromatic light elicited biomass accumulation, antioxidant activity, and secondary metabolites production in callus cultures of Operculina turpethum (L). Plant Cell Tissue Organ Cult 149:123–134. https://doi.org/10.1007/s11240-022-02274-9
Boccalandro HE, Giordano CV, Ploschuk EL, Piccoli PN, Bottini R, Casal JJ (2012) Phototropins but not cryptochromes mediate the blue light-specific promotion of stomatal conductance, while both enhance photosynthesis and transpiration under full sunlight. Plant Physiol 158:1475–1484. https://doi.org/10.1104/pp.111.187237
Casal JJ (2013) Photoreceptor signaling networks in plant responses to shade. Annu Rev Plant Biol 64:403–427. https://doi.org/10.1146/annurev-arplant-050312-120221
Cashmore AR, Jarillo JA, Wu YJ, Liu D (1999) Cryptochromes: blue light receptors for plants and animals. Science 284:760–765. https://doi.org/10.1126/science.284.5415.760
Chen M, Schwab R, Chory J (2003) Characterization of the requirements for localization of phytochrome B to nuclear bodies. Proc Natl Acad Sci USA 100:14493–14498. https://doi.org/10.1073/pnas.1935989100
Chen IGJ, Lee MS, Lin MK, Ko CY, Chang WT (2018) Blue light decreases tanshinone IIA content in Salvia miltiorrhiza hairy roots via gene regulation. J Photochem Photobiol B 183:164–171. https://doi.org/10.1016/j.jphotobiol.2018.04.013
Chen Y, Li T, Yan Q, Zhang Y, Zou J, Bian Z, Wen X (2019) UVA radiation is beneficial for yield and quality of indoor cultivated lettuce. Front Plant Sci 10:1563. https://doi.org/10.3389/fpls.2019.01563
Christie JM (2007) Phototropin blue-light receptors. Annu Rev Plant Biol 58:21–45. https://doi.org/10.1146/annurev.arplant.58.032806.103951
Darko EP, Heydarizadeh P, Schoefs B, Sabzalian MR (2014) Photosynthesis under artificial light: the shift in primary and secondary metabolism. Phil Trans R Soc B 369:20130243. https://doi.org/10.1098/rstb.2013.0243
Datta Gupta S, Jatothu B (2013) Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7:211–220. https://doi.org/10.1007/s11816-013-0277-0
de Castro KM, Batista DS, Fortini EA, Silva TD, Felipe SHS, Fernandes AM, Sousa RMDJ, Nascimento LSDQ, Campos VR, Grazul RM, Viccini LF, Otoni WC (2019) Photoperiod modulates growth, morphoanatomy, and linalool content in Lippia alba L. (Verbenaceae) cultured in vitro. Plant Cell Tissue Organ Cult 139:139–153. https://doi.org/10.1007/s11240-019-01672-w
Dillard CJ, German JB (2000) Phytochemicals: nutraceuticals and human health. J Sci Food Agricul 80:1744–1756. https://doi.org/10.1002/1097-0010(20000915)
Faccio G (2020) Plant complexity and cosmetic innovation. iScience 23:101358. https://doi.org/10.1016/j.isci.2020.101358
Fazal H, Abbasi BH, Ahmad N, Ali M, Ali S (2016) Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L. Plant Cell Tissue Organ Cult 124:573–581. https://doi.org/10.1007/s11240-015-0915-z
Findlay KM, Jenkins GI (2016) Regulation of UVR8 photoreceptor dimer/monomer photo-equilibrium in Arabidopsis plants grown under photoperiodic conditions. Plant Cell Environ 39:1706–1714. https://doi.org/10.1111/pce.12724
Fortini EA, Batista DS, de Castro KM, Silva TD, Felipe SHS, Correia LNF, Chagas K, Farias LM, Leite JPV, Otoni WC (2020) Photoperiod modulates growth and pigments of 20-hydroxyecdysone accumulation in Brazilian ginseng [Pfaffia Glomerata (Spreng.) Pedersen]. Plant Cell Tissue Organ Cult 142:595–611. https://doi.org/10.1007/s11240-020-01886-3
Fuglevand G, Jackson JA, Jenkins GI (1996) UV-B, UV-A, and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell 8:2347–23547. https://doi.org/10.1105/tpc.8.12.2347
Gabr AMM, Fayek NM, Mahmoud HM, El-Bahr MK, Ebrahim HS, Sytar O, El-Halawany AM (2022) Effect of light quality and media components on shoot growth, rutin and quercetin production from common buckwheat. ACS Omega 7:26566–26572. https://doi.org/10.1021/acsomega.2c02728
Gai QY, Feng X, Jiao J, Xu XJ, Fu JX, He XJ, Fu YJ (2023) Blue LED light promoting the growth, accumulation of high-value isoflavonoids and astragalosides, antioxidant response, and biosynthesis gene expression in Astragalus membranaceus (Fish.) Bunge hairy root cultures. Plant Cell Tissue Organ Cult 153: 511–523. https://doi.org/10.1007/s11240-023-02486-7
Gangappa SN, Botto JF (2016) The multifaceted roles of HY5 in plant growth and development. Mol Plant 9:1353–1365. https://doi.org/10.1016/j.molp.2016.07.002
Glassgen WE, Rose A, Madlung J, Koch W, Gleitz J, Seitz HU (1998) Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors. Planta 204:490–498
Guerriero G, Berni R, Munoz-Sanchez JA, Apone F, Abdel-Salam EM, Qahtan AA, Alatar AA, Cantini C, Cai G, Hausman JF, Siddiqui KS, Haenandez-Sotomayor SM, Faisal M (2018) Production of plant secondary metabolites: examples, tips and suggestions to biotechnologists. Genes 9:309. https://doi.org/10.3390/genes9060309
Hashim M, Ahmad B, Drouet S, Hano C, Abbasi BH, Anjum S (2021) Comparative effects of differential light sources on the production of key secondary metabolites in plant in vitro cultures. Plants 10:1521. https://doi.org/10.3390/plants10081521
Hectors K, Van Oevelen S, Geuns J, Guisez Y, Jansen MAK, Prinsen E (2014) Dynamic changes in plant secondary metabolites during UV acclimation in Arabidopsis thaliana. Physiol Plant 152:219–230. https://doi.org/10.1111/ppl.12168
Ho TT, Murthy HN, Park SY (2020) Methyl jasmonate induced oxidative stress and accumulation of secondary metabolites in plant cell and organ cultures. Int J Mol Sci 21:716. https://doi.org/10.3390/ijms21030716
Huche-Thelier L, Crespel L, Le Gourrierec J, Morel P, Sakr S, Leduc N (2015) Light singling and plant responses to blue and UV radiations-perspectives for applications in horticulture. Environ Exp Bot 121:22–38. https://doi.org/10.1016/j.envexpbot.2015.06.009
Hughes J (2013) Phytochromes cytoplasmic signaling. Annu Rev Plant Biol 64:377–402. https://doi.org/10.1146/annurev-arplant-050312-120045
Jan R, Asaf S, Numan M, Lubna, Kim KM (2021) Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy 11:968. https://doi.org/10.3390/agronomy11050968
Jenkins GI (2014) Structure and function of the UV-B photoreceptor UVR8. Curr Opin Struct Biol 29:52–57. https://doi.org/10.1016/j.sbi.2014.09.004
Jeong BR, Sivanesan I (2015) Direct adventitious shoot regeneration, in vitro flowering, fruiting, secondary metabolite content and antioxidant activity of Scrophularia takesimensis Nakai. Plant Cell Tissue Organ Cult 123:607–618. https://doi.org/10.1007/s11240-015-0864-6
Jiao Y, Lau OS, Deng XW (2007) Light-regulated transcriptional networks in higher plants. Nat Rev Genet 8:217–230. https://doi.org/10.1038/nrg2049
Jiao J, Gai QY, Yao LP, Niu LL, Zang YP, Fu YJ (2018) Ultraviolet radiation for flavonoid augmentation in Isatis tinctoria L. hairy root cultures mediated by oxidative stress and biosynthetic gene expression. Ind Crops Prod 118:347–354. https://doi.org/10.1016/j.indcrop.2018.03.046
Jing Y, Lin R (2020) Transcriptional regulatory network of the light signaling pathways. New Phytol 227:683–697. https://doi.org/10.1111/nph.16602
Kapoor S, Raghuvanshi R, Bhardwaj P, Sood H, Saxena S, Chaurasia OP (2018) Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola Imrbricata Edgew. J Photoch Photobiol B 183:258–265. https://doi.org/10.1016/j.jphotobiol.2018.04.018
Khan T, Abbasi BH, Khan MA (2018) The interplay between light, plant growth regulators and elicitors on growth and secondary metabolism in cell cultures of Fagonia Indica. J Photoch Photobiol B 185:153–160. https://doi.org/10.1016/j.jphotobiol.2018.06.002
Khonakdari MR, Rezadoost H, Heydari R, Mirjalili MH (2020) Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus Tazzeta L. (Amaryllidaceae), a potential source of galantamine. Plant Cell Tissue Organ Cult 142:187–199. https://doi.org/10.1007/s11240-020-01853-y
Khurshid R, Ullah MA, Tungmunnithum D, Drouet S, Shah M, Zaeem A, Hammed S, Hano C, Abbasi BH (2020) Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus cultures of Eclipta alba L. PLoS ONE 15:e0233963. https://doi.org/10.1371/journal.pone.0233963
Kim JY, Song JT, Seo HS (2017) COP1 regulates plant growth and development in response to light at the post-translational level. J Exp Bot 68:4737–4748. https://doi.org/10.1093/jxb/erx312
Kircher S, Kozma-Bognar L, Kim L, Adam E, Harter K, Schafer E, Nagy F (1999) Light quality-dependent nuclear import of the plant photoreceptors phytochrome A and B. Plant Cell 11:1445–1456. https://doi.org/10.1105/tpc.11.8.1445
Klimek-Szczykutowicz M, Prokopiuk B, Dziurka K, Pawlowska B, Ekiert H, Szopa A (2022) The influence of different wavelengths of LED light on the production of glucosinolates and phenolic compounds and the antioxidant potential in in vitro cultures of Nasturtium officinale (watercress). Plant Cell Tissue Organ Cult 149:113–122. https://doi.org/10.1007/s11240-021-02148-6
Kozlowska W, Matkowski A, Zielinska S (2022) Light intensity and temperature effect on Salvia Yangii (B. T. Drew) metabolic profile in vitro. Front Plant Sci 13:888509. https://doi.org/10.3389/fpls.2022.888509
Kubica P, Szopa A, Prokopiuk B, Komsta L, Pawlowska B, Ekiert H (2020) The influence of light quality on the production of bioactive metabolites – verbascoside, isoverbascoside, and phenolic acids and the content of photosynthetic pigments in biomass of Verbena officinalis L. cultured in vitro. J Photoch Photobiol B 203:111768. https://doi.org/10.1016/j.jphotobiol.2019.111768
Kumar SS, Arya M, Mahadevappa P, Giridhar P (2020) Influence of photoperiod on growth, bioactive compounds and antioxidant activity in callus cultures of Basella rubra L. J Photochem Photobiol B: Biol 111937. https://doi.org/10.1016/j.jphotobiol.2020.111937
Lange BM (2018) Commercial-scale tissue culture for the production of plant natural products: successes, failures and outlook. In: Schwab W, Lange BM, Wust M (eds) Biotechnology of natural products. Springer, Cham, pp 189–218. https://doi.org/10.1007/978-3-319-67903-7_8
Lazzarini LES, Bertolucci SKV, Pacheco FV, Santos JD, Silva ST, Carvalho AAD, Pinto JEBP (2018) Quality and intensity of light affect Lippia gracilis Schauer plant growth and volatile compounds in vitro. Plant Cell Tissue Organ Cult 135: 367–379. (2018) 135:367–379. https://doi.org/10.1007/s11240-018-1470-1
Li H, Lin Y, Chen X, Bai Y, Wang C, Xu X, Wang Y, Lai Z (2018) Effects of blue light on flavonoid accumulation linked to the expression of miR393, miR394 and miR395 in longan embryogenic calli. PLoS ONE 13:e0191444. https://doi.org/10.1371/journal.pone.0191444
Li QZ, Xu JX, Yang LY, Sun Y, Zhou XH, Zheng YH, Cai YM (2021) LED light quality affect growth, alkaloid contents, and expressions of Amaryllidaceae alkaloids biosynthetic pathway genes in Lycoris longituba. J Plant Growth Regl 41:257–270. https://doi.org/10.1007/s00344-021-10298-2
Liebelt DJ, Jordan JT, Doherty CJ (2019) Only a matter of time: the impact of daily and seasonal rhythms on phytochemicals. Phytochem Rev 18:1409–1433. https://doi.org/10.1007/s11101-019-09617-z
Liu CZ, Guo C, Wang YC, Ouyang F (2002) Effect of light irradiation on hairy root growth and artemisinin biosynthesis of Artemisia annua L. Process Biochem 38:581–585. https://doi.org/10.1016/S0032-9592(02)00165-6
Liu YL, Patra B, Pattanaik S, Wang Y, Yuan L (2019) GATA and phytochrome interacting factor transcription factors regulate light-induced vindoline biosynthesis in Catharanthus roseus. Plant Physiol 180:1336–1350. https://doi.org/10.1104/pp.19.00489
Lobiuc A, Vasilache V, Oroian M, Stoleru T, Burducea M, Pintilie O, Zamfirache MM (2017) Blue and red LED illumination improves growth and bioactive compounds contents in acyanic and cyanic Ocimum basilicum L. microgreens. Molecules 22:2111. https://doi.org/10.3390/molecules22122111
Loconsole D, Santamaria P (2021) UV lighting in horticulture: a sustainable tool for improving production quality and food safety. Horticulturae 7:9. https://doi.org/10.3390/horticulturae7010009
Lu Y, Zhang M, Meng X, Wan H, Zhang J, Tian J, Hao S, Jin K, Yao Y (2015) Photoperiod and shading regulate coloration and anthocyanin accumulation in the leaves of Malus crabapples. Plant Cell Tissue Organ Cult 121:619–532. https://doi.org/10.1007/s11240-015-0733-3
Manivannan A, Soundararajan P, Halima N, Ko CH, Jeong BR (2015) Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia Glutinosa cultured in vitro. Hort Environ Biotechnol 56:105–113
Marchev AS, Yardanova ZP, Georgiev MI (2020) Green (cell) factories for advanced production of plant secondary metabolites. Crit Rev Biotechnol 40:4443–4458
Moyo M, Koetle MJ, Van Staden J (2014) Photoperiod and plant growth regulator combinations influence growth and physiological responses in Pelargonium sidoides DC. Vitro Cell Dev Biol Plant 50:487–492. https://doi.org/10.1007/s11627-014-9594-4
Murthy HN, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue Organ Cult 118:1–16. https://doi.org/10.1007/s11240-014-0467-7
Murthy HN, Dandin VS, Paek KY (2016) Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem Rev 15:129–145. https://doi.org/10.1007/s11101-014-9391-z
Murthy HN, Joseph KS, Paek KY, Park SY (2023a) Anthraquinone production from cell and organ cultures of Rubis species: an overview. Metabolites 13:39. https://doi.org/10.3390/metabo13010039
Murthy HN, Joseph KS, Paek KY, Park SY (2023b) Production of anthraquinones from cell and organ cultures of Morinda species. Appl Microbiol Biotechnol 107:2061–2071. https://doi.org/10.1007/s00253-023-12440-4
Murthy HN, Joseph KS, Paek KY, Park SY (2023c) Bioreactor systems for micropropagation of plants: present scenario and future prospects. Front Plant Sci 14:1159588. https://doi.org/10.3389/fpls.2023.1159588
Murthy HN, Joseph KS, Paek KY, Park SY (2023d) Bioreactor configurations for adventitious root culture: recent advances toward the commercial production of specialized metabolites. Crit Rev Biotechnol 27:1–23. https://doi.org/10.1080/07388551.2023.2233690
Murthy HN, Joseph KS, Paek KY, Park SY (2023e) Suspension culture of somatic embryos for the production of high-value secondary metabolites. Physiol Mol Biol Plants 29:1153–1177. https://doi.org/10.1007/s12298-023-01365-x
Murthy HN, Joseph KS, Paek KY, Park SY (2023f) Nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites in cell and organ cultures: current status and future outlooks. Plant Growth Regul. https://doi.org/10.1007/s10725-023-01086-x
Murthy HN, Joseph KS, Paek KY, Park SY (2024a) Anthocyanin production from plant cell and organ cultures in vitro. Plants 13:117. https://doi.org/10.3390/plants13010117
Murthy HN, Joseph KS, Paek KY, Park SY (2024b) Production of biomass and bioactive compounds form cell and organ cultures of ginseng, He-shou-wu, purple coneflower, and St. John’s wort for the use in cosmetic industry. South Afr J Bot 164:334–344. https://doi.org/10.1016/j.sajb.2023.12.007
Najafabadi AS, Khanahamdi M, Ebrahimi M, Moradi K, Behroozi P, Noormohammadi N (2019) Effect of different quality of light on growth and production of secondary metabolites in adventitious root cultivation of Hypericum perforatum. Plant Sign Behav 14:e1640561. https://doi.org/10.1080/15592324.2019.1640561
Nhut DT, Huy NP, Tai NT, Nam NB, Luan VQ, Hien VT, Tung HT, Vinh BT, Luan TC (2015) Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis ha et Grushv. Biotechnol Biotechnol Equip 29:299–308. https://doi.org/10.1080/13102818.2014.1000210
Ocho-Villarreal M, Howat S, Hong SM, Jang M, Jin YW, Lee EY, Loake GJ (2016) Plant cell culture strategies for the production of natural products. BMB Rep 49:149–158. https://doi.org/10.5483/bmbrep.2016.49.3.264
Pan WS, Zheng LP, Tian H, Li WY, Wang JW (2014) Transcriptome responses involved in artemisinin production in Artemisia annua L. under UV-B radiation. J Photochem Photobiol B 140:292–300. https://doi.org/10.1016/j.jphotobiol.2014.08.013
Paradiso R, Proietti S (2022) Light-quality manipulation to control plant growth and photomorphogenesis in greenhouse horticulture: the state of the art and the opportunities of modern LED systems. J Plant Growth Regul 41:742–780. https://doi.org/10.1007/s00344-021-10337-y
Park YG, Kim MH, Yang JK, Chung YG, Choi MS (2003) Light-susceptibility of campotothecin production from in vitro cultures of Campotheca Acuminata Decne. Biotechnol Bioproc Eng 8:32–36. https://doi.org/10.1007/BF02932895
Park SY, Lee JG, Cho HS, Seong ES, Kim HY, Yu CY, Kim JK (2013) Metabolite profiling approach for assessing the effects of colored light-emitting diode lighting on the adventitious roots of ginseng (Panax ginseng C.A. Meyer). Plant Omics J 6:224–230
Pedmale UV, Huang SSC, Zander M, Cole BJ, Hetzel J, Ljung K, Reis PAB, Sridevi P, Nito K, Nery JR, Ecker JR (2016) Cryptochromes interact directly with PIFs to control plant growth in liming blue light. Cell 164:233–245. https://doi.org/10.1016/j.cell.2015.12.018
Peebles CAM, Shanks JV, San KY (2009) The role of the octadecanoid pathway in the production of terpenoid indole alkaloids in Catharanthus roseus hairy roots under normal and UV-B stress conditions. Biotechnol Bioeng 103:1248–1254. https://doi.org/10.1002/bit.22350
Pham VN, Kathare PK, Huq E (2018) Phytochromes and phytochrome interacting factors. Plant Physiol 178:1025–1038. https://doi.org/10.1104/pp.17.01384
Qin BF, Ma LL, Wang YX, Chen M, Lan XZ, Wu NB, Liao ZH (2014) Effect of acetylsalicylic acid and UV-B on gene expression and tropane alkaloid biosynthesis in hairy root cultures of Anisodus Luridus. Plant Cell Tissue Organ Cult 117:483–490. https://doi.org/10.1007/s11240-014-0454-z
Rai R, Meena RP, Smita SS, Shukla A, Rai SK, Pandey-Rai S (2011) UV-B and UV-C pre-treatments induce physiological changes and artemisinin biosynthesis in Artemisia annua L. – an antimalarial plant. J Photochem Photobiol B 105:216–225. https://doi.org/10.1016/j.jphotobiol.2011.09.004
Ramani S, Chelliah J (2007) UV-B-induced signaling events leading to enhanced -production of catharanthine in Catharanthus roseus cell suspension culture. BMC Plant Biol 7:61. https://doi.org/10.1186/1471-2229-7-61
Ramani S, Jayabaskaran C (2008) Enhanced catharanthine and vindoline production in suspension cultures of Catharanthus roseus by ultraviolet-B light. J Mol Singal 3:9. https://doi.org/10.1186/1750-2187-3-9
Reis A, Kleinowski AM, Klein FRS, Telles RT, Amarante LD, Braga EJB (2015) Light quality on the in vitro growth production of pigments in the genus Alternanthera. J Crop Sci Biotech 18:349–357. https://doi.org/10.1007/s12892-015-0074-0
Rosenfeldt G, Viana RM, Mootz HD, von Arnim AG, Batschauer A (2008) Chemically induced and light-independent cryptochrome photoreceptor activation. Mol Plant 1:4–14. https://doi.org/10.1093/mp/ssm002
Shin KS, Murthy HN, Heo JW, Paek KY (2004) Induction of betalain pigmentation in hairy roots of red beet under different radiation sources. Biol Plant 47:149–152. https://doi.org/10.1023/A:1027313805930
Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus embryos in bioreactor. Process Biochem 41:1179–1185. https://doi.org/10.1016/j.procbio.2005.12.015
Silva ST, Bertolucci SKV, Cunha SHBD, Lazzarini LES, Tavares MC, Pinto JEBP (2017) Effect of light and natural ventilation systems on the growth parameters and carvacrol content in the in vitro cultures of Plectranthus amboinicus (Lour.) Spreng. Plant Cell Tissue Organ Cult 129:501–510. https://doi.org/10.1007/s11240-017-1195-6
Song G, Jia M, Chen K, Kong X, Khattak B, Xie C, Li A, Mao L (2016) CRISPR/Cas9: a powerful tool for crop genome editing. Crop J 4:75–82. https://doi.org/10.1016/j.cj.2015.12.002
Stevens C, Liu J, Khan VA, Lu JY, Kabwe MK, Wilson CL, Igwegbe ECK, Chalutz E, Droby S (2004) The effects of low-dose ultraviolet light-C treatment on polygalacturonase activity, delay ripening, Rhizopus soft rot development of tomatoes. Crop Prot 23:551–554. https://doi.org/10.1016/j.cropro.2003.10.007
Szopa A, Ekiert H (2016) The importance of applied light quality on the production of lignans and phenolic acids in Schisandra chinensis (Turez.) Baill. Cultures in vitro. Plant Cell Tissue Organ Cult 127: 115–121. https://doi.org/10.1007/s11240-016-1034-1
Takshak S, Agrawal SB (2019) Defense potential of secondary metabolites in medicinal plants under UV-B stress. J Photochem Photobiol B Biol 193:51–88. https://doi.org/10.1016/j.jphotobiol.2019.02.002
Tusevski O, Stanoeva JP, Stefova M, Simic SG (2013) Phenolic profile of dark-grow and photoperiod-exposed Hypericum Pefroratum L. hairy root cultures. Sci World J ID 602752. https://doi.org/10.1155/2013/602752
Ullah MA, Tungmunnithaum D, Garros L, Drouet S, Hano C, Abbasi BH (2019) Effect of ultraviolet-C radiation and melatonin stress on biosynthesis of antioxidant and antidiabetic metabolites production in in vitro callus cultures of Lepidium sativum L. Int J Mol Sci 20:1787. https://doi.org/10.3390/ijms20071787
Vanhaelewyn L, Van Der Straeten D, De Coninck B, Vandenbussche F (2020) Ultraviolet radiation from a plant perspective: the plant-microorganism context. Front Plant Sci 11:597642. https://doi.org/10.3389/fpls.2020.597642
Verdaguer D, Jansen MAK, Llorens L, Morales LO, Neugart S (2017) UV-A radiation effects on higher plants: exploring the known unknown. Plant Sci 255:72–81. https://doi.org/10.1016/j.plantsci.2016.11.014
Wang Y, Zhang H, Zhao B, Yuan X (2001) Improved growth of Artemisia annua L hairy roots and artemisinin production under red light conditions. Biotechnol Lett 23:1971–1973. https://doi.org/10.1023/A:1013786332363
Wang CH, Zheng LP, Tian H, Wang JW (2016) Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots. J Photochem Phtobiol B 159:93–100. https://doi.org/10.1016/j.jphotobiol.2016.01.012
Wawrosch C, Zotchev SB (2021) Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook. Appl Microbiol Biotechnol 105:5549–6668. https://doi.org/10.1007/s00253-021-11539-w
Weller JL, Kendrick RE (2015) Photomorphogenesis and photoperiodism in plants. In: Bjorn LO (ed) Photobiology: the science of light and life. Springer, New York, USA, pp 299–321. https://doi.org/10.1007/978-1-4939-1468-5_19
Wu CH, Murthy HN, Hahn EJ, Paek (2007) Enhanced production of caftaric acid, chlorogenic acid and cichoric acid in suspension cultures of Echinacea purpurea by manipulation of incubation temperature and photoperiod. Biochem Eng J 36:301–303. https://doi.org/10.1016/j.bej.2007.02.024
Wu D, Hu Q, Yan Z, Chen W, Yan C, Huang X, Zhang J, Yang P, Deng H, Wang J, Deng XW, Shii Y (2012) Structural basis of ultraviolet B perception by UVR8. Nature 484:214–219. https://doi.org/10.1038/nature10931
Xu D (2019) COP1 and BBXs-HY5-mediated light signal transduction in plants. New Phytol 228:1748–1753. https://doi.org/10.1111/nph.16296
Yadav A, Sing D, Lingwan M, Yadukrishnan P, Maskapalli SK, Datta S (2020) Light singling an UV-B-mediated plant growth regulation. J Integr Plant Biol 62:1270–1292. https://doi.org/10.1111/jipb.12932
Yousefzadi M, Sharifi M, Behmanesh M, Ghasempour A, Moyano E, Palazon J (2012) The effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture. Plant Physiol Biochem 56:41–46. https://doi.org/10.1016/j.plaphy.2012.04.010
Yu KW, Murthy HN, Hahn EJ, Paek KY (2005) Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality. Biochem Eng J 23:53–56. https://doi.org/10.1016/j.bej.2004.07.001
Zahir A, Abbasi BH, Adil M, Anjum S, Zia M, Ihasa-ul H (2014) Synergistic effects of drought stress and photoperiods on phenology and secondary metabolites of Silybum marianum. Appl Biochem Biotechnol 174:163–707. https://doi.org/10.1007/s12010-014-1098-5
Zhang L, Allen LH, Vaughan MM, Hauser BA, Boote KJ (2014) Solar ultraviolet radiation exclusion increases soybean internode lengths and plant height. Agric Meteor 184:170–178. https://doi.org/10.1016/j.agrformet.2013.09.011
Zhang S, Zhang L, Zou H, Qiu L, Zheng Y, Yang D, Wang Y (2021) Effect of light on secondary metabolite biosynthesis in medicinal plants. Front Plant Sci 12:781236. https://doi.org/10.3389/fpls.2021.781236
Acknowledgements
Hosakatte Niranjana Murthy is thankful for the “Brain Pool” (BP) program (Grant No. 2022H1D3A2A02056665). This work was supported by the National Research Foundation of Korea (NRF) Grant (MSIT) (No. NRF-2020R1A2C2102401), Korea, and the Technology Innovation Program (Grant Number P0018148) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
Author information
Authors and Affiliations
Contributions
HNM: conceptualization; HNM, KSJ, KYP: investigation, data curation, formalization; SYP: resources, validation; HNM, KSJ, KYP, SYP: writing, review, and editing. All the authors have read and agreed to the published version of this manuscript.
Corresponding authors
Ethics declarations
Conflicts of interest
The authors declare no potential conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Murthy, H.N., Joseph, K.S., Paek, K.Y. et al. Light as an elicitor for enhanced production of secondary metabolites in plant cell, tissue, and organ cultures. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01139-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10725-024-01139-9