Abstract
The aim of the current study is to investigate the influence of light intensity, quality of light and alternative membrane sytems on the growth and headspace-GC/MS chemical analysis of Plectranthus amboinicus cultivated in vitro. Nodal segments were grown under light intensities (26, 51, 69, 94 and 130 µmol m−2 s−1) provided by cool-white fluorescent lamps. Apical segments were grown under light-emitting diodes blue; red; 1 blue/2.5 red; 2.5 blue/1 red; 1 blue/1 red and white fluorescent lamps. Apical and nodal segments were grown under alternative membrane and membrane-free systems. One, two or four PTFE membranes were used on the lid of the culture vessel. The membranes provided natural ventilation and worked as filters. The results have shown significant differences in the growth and carvacrol content, as well as in the content of carvacrol precursors (γ-terpinene and p-cymene) in different treatments. Among all tested light intensities, the significant increase in the dry weight and in the carvacrol content of plantlets derived from the nodal segments was recorded at 69 µmol m−2 s−1. The monochromatic red led to greater shoot length and higher dry weight in plantlets derived from the apical segments, as well as to carvacrol accumulation greater than that provided by the fluorescent lamps. The culture vessel enclosure by one and two membranes led to higher dry weight in plantlets derived from the apical and nodal segments, respectively. They also showed higher carvacrol content. Thus, it is possible optimizing the growth and carvacrol content in P. amboinicus cultivated in vitro by adjusting these environmental parameters.
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Abbreviations
- GC/MS:
-
Gas chromatography/mass spectrometry
- LED:
-
Light emitting diodes
- R:
-
Red
- B:
-
Blue
- F:
-
Fluorescent
- NMS:
-
No membrane system
- AMS:
-
Alternative membrane system
- AMS1:
-
Alternative membrane system with one filter
- AMS2:
-
Alternative membrane system with two filters
- AMS4:
-
Alternative membrane system with four filters
- PTFE:
-
Polytetrafluoroethylene
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Acknowledgements
The authors would like to thank the National Council for Scientific and Technological Development (CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico), the Coordination for the Improvement of Higher Education Personnel (CAPES—Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and the Minas Gerais State Research Foundation (FAPEMIG—Fundação de Pesquisa do Estado de Minas Gerais) for the financial support (scholarships and research grants).
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Silva, S.T., Bertolucci, S.K.V., da Cunha, S.H.B. et al. Effect of light and natural ventilation systems on the growth parameters and carvacrol content in the in vitro cultures of Plectranthus amboinicus (Lour.) Spreng. Plant Cell Tiss Organ Cult 129, 501–510 (2017). https://doi.org/10.1007/s11240-017-1195-6
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DOI: https://doi.org/10.1007/s11240-017-1195-6