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Translationally controlled tumor protein GmTCTP interacts with GmCDPKSK5 in response to high temperature and humidity stress during soybean seed development

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Abstract

The developing seed of soybean is susceptible to high temperature and humidity (HTH) stress, resulting in pre-harvest seed deterioration in the field. Many genes are found to respond to the stress. Based on our previous proteomics study, an HTH-responsive gene, GmCDPKSK5, was isolated from soybean seed. GmCDPKSK5 encodes a cytoplasm- and membrane-associated protein, which belongs to Group I of the CDPK family. By yeast two-hybrid (Y2H) from soybean seed cDNA library, GmTCTP was screened as a GmCDPKSK5-interacting protein. The interaction between GmCDPKSK5 and GmTCTP was further verified using bimolecular fluorescence complementation and GST pull down assays. Expression levels of both GmCDPKSK5 and GmTCTP were induced by HTH stress in soybean seed. Our results indicated that GmCDPKSK5 and GmTCTP interact with each other and may function in responses to HTH stress in soybean developing seed.

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Abbreviations

BiFC:

Bimolecular fluorescence complementation

CDPK:

Calcium dependent protein kinases

CV:

Cultivar

GFP:

Green fluorescent protein

GST:

Glutathione S-transferase

HTH:

High temperature and humidity

ORF:

Open reading frame

PCR:

Polymerase chain reaction

qRT-PCR:

Quantitative reverse transcription PCR

TCTP:

Translationally controlled tumor protein

Y2H:

Yeast two-hybrid

YFP:

Yellow fluorescent protein

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Acknowledgements

We gratefully acknowledge the partial financial support from the projects supported by the Shanghai Committee of Agriculture, China [Hu (2013), No. 1–2], The National Natural Science Foundation of China (30971840, 31171572, 31371711, 31671772), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20100097110030, 20120097110025), and the Agriculture Science and Technology Fund of the Ministry of Science and Technology, China (02EFN216901241, 04EFN213100094) for this research.

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  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Shuang Wang, Yuan Tao, Yali Zhou, Juan Niu, Yingjie Shu, Xingwang Yu, Sushuang Liu, Ming Chen & Hao Ma

  2. Crop Science Department, North Carolina State University, Raleigh, NC, 27695, USA

    Xingwang Yu

  3. Animal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai, 201106, People’s Republic of China

    Weihong Gu

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  1. Shuang Wang
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Wang, S., Tao, Y., Zhou, Y. et al. Translationally controlled tumor protein GmTCTP interacts with GmCDPKSK5 in response to high temperature and humidity stress during soybean seed development. Plant Growth Regul 82, 187–200 (2017). https://doi.org/10.1007/s10725-017-0250-y

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