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GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean

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Abstract

Key message

GmSBH1 involves in response to high temperature and humidity stress.

Abstract

Homeobox transcription factors are key switches that control plant development processes. Glycine max H1 Sbh1 (GmSBH1) was the first homeobox gene isolated from soybean. In the present study, the full ORF of GmSBH1 was isolated, and the encoded protein was found to be a typical class I KNOX homeobox transcription factor. Subcellular localization and transcriptional activation assays showed that GmSBH1 is a nuclear protein and possesses transcriptional activation activity in the homeodomain. The KNOX1 domain was found to play a clear role in suppressing the transcriptional activation activity of GmSBH1. GmSBH1 showed different expression levels among different soybean tissues and was involved in response to high temperature and humidity (HTH) stress in developing soybean seeds. The overexpression of GmSBH1 in Arabidopsis altered leaf and stoma phenotypes and enhanced seed tolerance to HTH stress. Overall, our results indicated that GmSBH1 is involved in growth, development, and enhances tolerance to pre-harvest seed deterioration caused by HTH stress in soybean.

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Abbreviations

2-DE:

2-dimensional electrophoresis

BF:

Bright field

GFP:

Green fluorescent protein

GmSBH1:

Glycine max H1 Sbh1

HD:

Homeodomain

HTH:

High temperature and humidity

MS:

Medium, Murashige and Skoog medium

NLS:

Nuclear localization signal

ORF:

Open reading frames

qRT-PCR:

Quantitative reverse transcription PCR

RH:

Relative humidity

RT-PCR:

Reverse transcription PCR

SAM:

Shoot apical meristem

SD:

Synthetic dropout

SEM:

Scanning electron microscopy

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Acknowledgments

This research was financially supported partially by the National Natural Science Foundation of China (31101212, 30971840, 31371711 and 31171572), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20120097110025), the Excellent Young Talents Program of Anhui Province Colleges and Universities of China (2012 SQRL143), the Shanghai Committee of Agriculture, China (Hu 2013, No. 1-2), and the Shanghai Committee of Science and Technology, China (093919N1400, 12391900900). We thank Dr. Kathryn Winglee (Department of Bioinformatics and Genomics, University of North Carolina, USA) for his excellent language correction.

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    Authors and Affiliations

    1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

      Yingjie Shu, Yuan Tao, Shuang Wang, Liyan Huang, Xingwang Yu, Zhankui Wang, Ming Chen & Hao Ma

    2. College of Agriculture, Anhui Science and Technology University, Fengyang, 233100, China

      Yingjie Shu

    3. Animal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai, 201106, People’s Republic of China

      Weihong Gu

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    1. Yingjie Shu
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    2. Yuan Tao
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    Correspondence to Hao Ma.

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    Communicated by Z.-Y. Wang.

    Authors Yingjie Shu and Yuan Tao contributed equally to this work.

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    Shu, Y., Tao, Y., Wang, S. et al. GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean. Plant Cell Rep 34, 1927–1937 (2015). https://doi.org/10.1007/s00299-015-1840-7

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