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Linked activation of cell division and oxidative stress defense in alfalfa leaf protoplast-derived cells is dependent on exogenous auxin

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Abstract

The activation of cell division and oxidative stress responses has been investigated in the case of leaf protoplast-derived cells. Initiation of protoplast culture was found to be associated with oxidative stress as indicated by the rate of H2O2 release into the medium and/or by catalase and ascorbate peroxidase activities. Both cell division frequency and the above stress-related parameters were dependent on the exogenous auxin (2,4-dichlorophenoxyacetic acid, 2,4-D) concentrations used. In addition, the well known oxidative stress-inducing agent paraquat (1 μM) could promote cell division at suboptimal auxin concentration but not in the absence of exogenous auxin. The H2O2 scavenger dimethylthiourea and the NADPH oxidase inhibitor diphenyleneiodonium inhibited not only the activation of cellular defense reactions but cell division as well. Based on the above experimental observations, it is suggested that exogenous auxin (2,4-D) enhances cellular defense reactions in parallel with cell division activation.

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Acknowledgements

This work was partly supported by the “Országos Tudományos Kutatási Alap (OTKA)” grant #T034818 and the grant BIO-00062/2000 from the Ministry of Education, Hungary. A. F. is thankful for the János Bólyai Research Fellowship for its support.

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  1. Taras P. Pasternak

    Present address: Institute of Biologie II, Ludwig-Albert University, Sonnerster 5, 79104, Freiburg, Germany

Authors and Affiliations

  1. Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62., H-6726, Szeged, P.O. Box 521, H-6701, Szeged, Hungary

    Taras P. Pasternak, Krisztina Ötvös, Mónika Domoki & Attila Fehér

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  1. Taras P. Pasternak
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  2. Krisztina Ötvös
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  3. Mónika Domoki
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  4. Attila Fehér
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Correspondence to Attila Fehér.

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Pasternak, T.P., Ötvös, K., Domoki, M. et al. Linked activation of cell division and oxidative stress defense in alfalfa leaf protoplast-derived cells is dependent on exogenous auxin. Plant Growth Regul 51, 109–117 (2007). https://doi.org/10.1007/s10725-006-9152-0

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  • DOI: https://doi.org/10.1007/s10725-006-9152-0

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