Abstract
Assam lemon represents the core table fruit and a very important part of the food regime of Northeast India. It stands out from the other lemons owing to its distinctive aroma and flavour. Northeast India being the cradle to the various Citrus species, we were interested in cataloging the genetic diversity accumulated by this cultivar over the last 67 years of its existence. For this, Assam lemon (510 samples) collected from 97 different locations in 16 districts of Assam were analyzed using 12 inter simple sequence repeats, which showed 37,607 DNA amplicons among which 31,997 (85.08%) indicate polymorphism. The average polymorphism information content was 0.89, whereas total gene diversity among the populations (HT) and within-population (Hs) were 0.14 (0.03) and 0.07 (0.01), respectively. The mean coefficient of gene differentiation was high (GST = 0.52), resulting in a moderate gene flow (Nm = 0.46), indicating genetic differentiation among the populations of Assam lemon. The analysis of molecular variance specified that 84% molecular variance was observed among the population. In contrast to the samples from other districts, the populations from Tinsukia, and Dhemaji districts of Assam were genetically similar to the original stock conserved at Kahikuchi, as evident from the diversity analysis.
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Acknowledgements
The authors are indebted to Gauhati University for providing the technical facility. The authors also acknowledge Department of Biotechnology, Government of India under Task Force scheme for providing the financial aid vide sanction number BT/PR21144/BPA/118/203/2016.
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This work was supported by Department of Biotechnology, Government of India under Task Force (sanction number BT/PR21144/BPA/118/203/2016).
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Original draft preparation, investigation, acquisition of data, analysis and interpretation of data was performed by Raja Ahmed. Writing and substantial revision of the article was done by SA. The article was reviewed by AD and KB. The article was also critically reviewed by KK. The conception, study design, supervison, critically reviewed and revised by SB. All authors read and approved the final manuscript. The authors also declare no conflict of interest.
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Ahmed, R., Akhtar, S., Das, A. et al. Delineating the degree of genetic divergence within Assam lemon (Citrus limon ‘Assam lemon’) accessions in Assam, India. Genet Resour Crop Evol 70, 2785–2799 (2023). https://doi.org/10.1007/s10722-023-01606-8
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DOI: https://doi.org/10.1007/s10722-023-01606-8