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Evaluating Genetic Diversity Within Genus Jasminum L. (Oleaceae) Using Intersimple Sequence Repeats (ISSR) Marker

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Jasmines are an important group of plants extensively used in the perfumery industry, preparation of garland and for ornamental purposes. To analyze the genetic potential of the group, the authors evaluated its genetic diversity. Intersimple sequence repeats (ISSR) markers were used to evaluate genetic diversity among 40 accessions of 23 Jasminum species including six endemic species. The present study is the first report of the efficacy of 10 shortlisted ISSR markers out of 30 primers screened. Among 23 Jasminum species, J. bignoniaceum revealed maximum genetic diversity and Shannon’s Index. Results revealed that the accessions of Jasminum species are grouped into three major clades, showing close agreement with the morphological relatedness. The diversity analysis also showed that the members of section Alternifolia are the ancestor to the rest of the Jasminum spp. The study provides direction for the future population study on Jasminum spp. This is the first report on the genetic diversity assessment of Indian Jasminum spp. incorporating approximately 50% species.

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Acknowledgements

The authors are thankful to UGC, New Delhi, for financial support (#32-409/2006 (SR), and to Kerala Forest Department for permission to collect Jasminum spp. from forest areas in Kerala. NJ, RY, DV greatly acknowledge the help rendered by the Principals of respective Sree Narayana Colleges at Chempazhanthy and Kollam for providing research facilities.

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Authors and Affiliations

  1. Department of Botany, Sree Narayana College, Kollam, Kerala, India

    Regy Yohanan

  2. Department of Botany, Fatima Mata National College (Autonomous), Kollam, Kerala, India

    Nirmala J. Jeyarani

  3. Department of Botany, Sree Narayana College, Chempazhanthy, Thiruvananthapuram, Kerala, India

    V. Devipriya

  4. Department of Botany, University of Delhi, Delhi, 110007, India

    Shabir A. Rather, Shruti Kasana, Julie Thakur, Mayank D. Dwivedi & Arun K. Pandey

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  1. Regy Yohanan
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  2. Nirmala J. Jeyarani
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  3. V. Devipriya
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  4. Shabir A. Rather
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  5. Shruti Kasana
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  6. Julie Thakur
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  7. Mayank D. Dwivedi
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  8. Arun K. Pandey
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Contributions

AKP conceived the idea and provided all the laboratory facilities at the Department of Botany, University of Delhi and also helped in designing the experiments and finalizing the manuscript. RY collected specimens for herbarium preparation and preserved leaf materials for DNA extraction and also helped in experimental work and manuscript preparation. NJJ helped in collection of materials and manuscript writing. DV helped in experimental design, manuscript preparation, and final discussion. SAR helped in experimental work. SK helped in analysis of data and manuscript writing. JT helped in experimental work, data analysis, and manuscript writing. MDD helped in experimental design, laboratory work, interpretation of data, and manuscript writing.

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Correspondence to Arun K. Pandey.

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Significance Statement

Genus Jasminum is an economically important oil-yielding taxa. Despite its high economic value, no molecular study was done to assess the genetic diversity within the genus in India. ISSR-PCR technique will help to estimate the genetic similarities and evaluate relationships between available accessions of Jasminum species in India.

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Yohanan, R., Jeyarani, N.J., Devipriya, V. et al. Evaluating Genetic Diversity Within Genus Jasminum L. (Oleaceae) Using Intersimple Sequence Repeats (ISSR) Marker. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 531–540 (2020). https://doi.org/10.1007/s40011-019-01124-7

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  • DOI: https://doi.org/10.1007/s40011-019-01124-7

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