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Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

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Abstract

Maintaining germplasm genetic integrity is a key objective of long-term ex situ conservation. Periodic regeneration, performed on limited plots with small number of individuals, increases the risk of genetic drift and genetic diversity changes. In this study, six accessions of white flowered, dry seed pea varieties (Pisum sativum L. subsp. sativum var. sativum): Bohatýr, Klatovský zelený, Hanák, Moravský hrotovický krajový, Raman and Viktoria-75 and four accessions of colour flowered, fodder pea (P. sativum subsp. sativum var. arvense (L.) Poiret: Arvika, Český banán, Moravská krajová and Niké, representing Czechoslovak varieties and landraces, bred over the last 40–80 years, were analyzed using ten microsatellite locus specific markers. Each accession was represented by 20 individual seeds of two temporally different samples, spanning the period of 20 or 40 years. Together with intra-accession variation (except of cv. Hanák), evidence of genetic changes, e.g. differences in allele frequencies as well as genetic composition of sample, was detected in six out of ten accessions (Arvika, Bohatýr, Český banán, Moravský hrotovický krajový, Moravská krajová and Raman). Evidence of genetic erosion was found in three accessions (Český banán, Moravský hrotovický krajový and Raman), while in another three (Arvika, Bohatýr and Moravská krajová) the level of diversity was found to have increased. Moreover in three samples of Bohatýr (2004) and Klatovský zelený (1963 and 2004), low levels of heterozygosity was detected. These results demonstrate that in pea, a self-pollinating and highly homozygous plant, the danger of the loss of genetic integrity exists. These findings are significant for long-term ex situ germplasm management.

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References

  • Ambrose MJ (1995) From near east centre of origin the prized pea migrates throughout world. Diversity 11:118–119

    Google Scholar 

  • Baranger A, Aubrey G, Aran G, Laine AL, Deniot G, Potier J, Burstin J (2004) Genetic diversity within Pisum sativum using protein and PCR-based markers. Theor Appl Genet 108:1309–1321

    Article  PubMed  CAS  Google Scholar 

  • Baur E (1914) Die Bedeutung der primitiven Kulturrassen und der wilden Verwandten unserer Kulturpflanzen für die Pflanzenzüchtung. Jb Deut Landw Ges 29:104–109

    Google Scholar 

  • Börner A, Chebotar C, Korzun V (2000) Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance. Theor Appl Genet 100:494–497

    Article  Google Scholar 

  • Breese EL (1989) Regeneration and multiplication of germplasm resources in seed genebanks: the scientific background. International Board for Plant Genetic Resources, Rome

    Google Scholar 

  • Brown AHD (1989) Core collections: a practical approach to genetic resources management. Genome 31:818–824

    Google Scholar 

  • Brown AHD, Brubaker CL, Grace JP (1997) Regeneration of germplasm samples: wild versus cultivated plant species. Crop Sci 37:7–13

    Article  Google Scholar 

  • Chebotar S, Roder MS, Korzun V, Borner A (2002) Genetic integrity of ex situ genebank collections. Cell Mol Biol Lett 7:437–444

    PubMed  CAS  Google Scholar 

  • Chwedorzewska KJ, Bednarek PT, Puchalski J, Krajewski P (2002) AFLP-profiling of long term stored and regenerated rye genebank samples. Cell Mol Biol Lett 7:457–463

    PubMed  CAS  Google Scholar 

  • Dostálová R, Seidenglanz M, Griga M (2005) Simulation and assessment of possible environmental risks associated with release of genetically modified peas (Pisum sativum L.) into environment in Central Europe. Czech J Genet Plant Breed 41:51–63

    Google Scholar 

  • Enjabert J, Goldringer I, Paillard S, Brabant P (1999) Molecular markers to study genetic drift and selection in wheat populations. J Exp Botany 50:283–290

    Article  Google Scholar 

  • Esquinas-Alcázar J (2005) Protecting crop genetic diversity for food security: political, ethical and technical challenges. Nat Rev Genet 6:946–953

    Article  PubMed  Google Scholar 

  • FAOSTAT (2007) Databases Query, http://faostat.fao.org/

  • Figliuolo G, Mazzeo M, Greco I (2007) Temporal variation of diversity in Italian durum wheat germplasm. Genet Resour Crop Evol 54:615–626

    Article  Google Scholar 

  • Frankel OH, Brown AHD (1984) Current plant genetic resources—a critical appraisal. In: Chopra VL, Joshi BC, Sharma RP, Bansal HC (eds) Genetics: new Frontiers, vol IV. Oxford & IBH Publ, New Delhi, pp 1–11

    Google Scholar 

  • Genebank Standards (1994) Food and Agriculture Organization of the United Nations, Rome, International Plant Genetic Resources Institute, Rome

  • Goudet J (1995) FSTAT (ver.1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Griga M, Smýkal P, Dostálová R, Seidenglanz M (2008) Field assessment of outcrossing rate in pea (Pisum sativum L.), a cleistogamous crop, as a background for release of genetically modified pea into environment. In: 10th international symposium on biosafety of genetically modified organisms, symposium handbook: biosafety research of GMOs: past achievements and future challenges. Wellington, New Zealand, p 110

  • Gugerli F, Brodbeck S, Holderegger R (2008) Insertions–deletions in a microsatellite flanking region may be resolved by variation in stuttering patterns. Plant Mol Biol Rep 26:255–262

    Article  CAS  Google Scholar 

  • Horáček J, Griga M, Smýkal P, Hýbl M (2009) Effect of environmental and genetic factors on the stability of pea (Pisum sativum L.) isozyme and DNA markers. Czech J Genet Plant Breed 45:57–71

    Google Scholar 

  • ICRISAT (1995) Consultation meeting on the regeneration of seed crops and their wild relatives. ICRISAT, Hyderabad, India

    Google Scholar 

  • Jing RC, Knox MR, Lee JM, Vershinin AV, Ambrose M, Ellis THN, Flavell AJ (2005) Insertional polymorphism and antiquity of PDR1 retrotransposon insertions in Pisum species. Genetics 171:741–752

    Article  PubMed  CAS  Google Scholar 

  • Le Clerc V, Briard M, Granger J, Delettre J (2003) Genebank biodiversity assessments regarding optimal sample size and seed harvesting techniques for the regeneration of carrot accessions. Biodiv Conserv 12:2227–2336

    Article  Google Scholar 

  • Lehmann CO, Mansfeld R (1957) Zur Technik der Sortimentserhaltung. Die Kulturpflanze 5:108–138

    Article  Google Scholar 

  • Lia VV, Bracco M, Gottlieb AM, Poggio L, Confalonieri VA (2007) Complex mutational patterns and size homoplasy at maize microsatellite loci. Theor Appl Genet 115:981–991

    Article  PubMed  CAS  Google Scholar 

  • Loridon K, McPhee K, Morin J, Dubreuil P, Pilet-Nayel ML, Aubert G et al (2005) Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor Appl Genet 111:1022–1031

    Article  PubMed  CAS  Google Scholar 

  • Malysheva-Otto L, Ganal MW, Law JR, Reeves JC, Röder MS (2007) Temporal trends of genetic diversity in European barley cultivars (Hordeum vulgare L.). Mol Breed 20:309–322

    Article  Google Scholar 

  • Mendel JG (1866) Versuche über Pflanzenhybriden. Verhandlungen des naturforschenden Vereines in Brünn, Bd. IV für das Jahr 1865

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  CAS  Google Scholar 

  • Otto SP, Whitlock MC (1997) The probability of fixation in populations of changing size. Genetics 146:723–733

    PubMed  CAS  Google Scholar 

  • Parzies HK, Spoor W, Ennos RA (2000) Genetic diversity of barley landrace accessions (Hordeum vulgare ssp. vulgare) conserved for different length of time in ex situ gene banks. Heredity 84:476–486

    Article  PubMed  CAS  Google Scholar 

  • Pavelková A, Moravec J, Hájek D, Bareš I, Sehnalová J (1986) Descriptor list of the genus Pisum L. RICP Prague—Ruzyně. Genové zdroje 32:46

    Google Scholar 

  • Reynolds J, Weir BS, Cockerham C (1983) Estimation of the coancestry coefficient: basis for a short-term genetic distance. Genetics 105:767–776

    PubMed  CAS  Google Scholar 

  • Roberts EH, Ellis RH (1984) The implications of the deterioration of orthodox seeds during storage for genetic resources conservation. In: Holden JHW, Williams JT (eds) Crop genetic resources: conservation and evaluation. IBPGR, George Allen and Unwin Ltd. Rome, International Plant Genetic Resources Institute, Rome, pp 19–37

    Google Scholar 

  • Russell JR, Ellis RP, Thomas WTB, Waugh R, Provan J, Booth A, Fuller J, Lawrence P, Young G, Powell W (2000) A retrospective analysis of spring barley germplasm development from ‘foundation genotypes’ to currently successful cultivars. Mol Breed 6:553–568

    Article  Google Scholar 

  • Sackville HNR, Chorlton KH (1997) Regeneration of accessions in seed collections: a decision guide. Handbook for genebanks No. 5. International Plant Genetic Resources Institute, Rome

    Google Scholar 

  • Schindler F (1890) Welches Werthverhältniss besteht zwischen den Landrassen landwirthschaftlicher Kulturpflanzen und den sogenannten Züchtungsrassen ? Internationaler land- und forstwirtschaftlicher Congress zu Wien 1890. Section I: Landwirthschaft. Subsection: Pflanzenbau. Frage 5. Heft 13:19–24

    Google Scholar 

  • Smýkal P (2006) Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification. J Appl Genet 47:221–230

    Article  PubMed  Google Scholar 

  • Smýkal P, Horáček J, Dostálová R, Hýbl M (2008a) Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers. J Appl Genet 49:155–166

    Article  PubMed  Google Scholar 

  • Smýkal P, Hýbl M, Corander J, Jarkovský J, Flavell A, Griga M (2008b) Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theor Appl Genet 117:413–424

    Article  PubMed  Google Scholar 

  • Smýkal P, Kalendar R, Ford R, Macas J, Griga M (2009) Evolutionary conserved lineage of Angela-family retrotransposons as a genome-wide microsatellite repeat dispersal agent. Heredity 103:157–167

    Article  PubMed  Google Scholar 

  • Soengas P, Cartea E, Lema M, Velasco P (2009) Effect of regeneration procedures on the genetic integrity of Brassica oleracea accessions. Mol Breed 23:389–395

    Article  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed bank and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    Article  PubMed  CAS  Google Scholar 

  • UPOV-BMT: BMT/36/10. (2002) Progress report of the 36th session of the technical committee, the technical working parties and working group on biochemical and molecular techniques and DNA-profiling in particular. Geneva

  • van Hintum TJL, Van De Wiel CCM, Visser DL, Van Treuren R, Vosman B (2007) The distribution of genetic diversity in a Brassica oleracea gene bank collection related to the effects on diversity of regeneration, as measured with AFLPs. Theor Appl Genet 114:777–786

    Article  PubMed  CAS  Google Scholar 

  • von Proskowetz E (1890) Welches Werthverhältniss besteht zwischen den Landrassen landwirthschaftlicher Kulturpflanzen und den sogenannten Züchtungsrassen ? Internationaler land- und forstwirtschaftlicher Congress zu Wien 1890. Section I: Landwirthschaft. Subsection: Pflanzenbau. Frage 5. Heft 13:3–18

    Google Scholar 

  • Wright S (1965) The interpretation of population structure by F-statistics with special regards to systems of mating. Evolution 19:395–420

    Article  Google Scholar 

  • Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian J Botany 129:157

    Google Scholar 

  • Zohary D, Hopf M (2000) Domestication of plants in the Old World. Oxford University Press, Oxford

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Ministry of Education of Czech Republic, MSM 2678424601 project. Excellent technical assistance of Mrs. E. Fialová, valuable discussion and manuscript improvement by Dr. C. Coyne, USDA-ARS Pullman and Dr. Mike Ambrose, JIC Norwich UK are greatly acknowledged.

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Authors and Affiliations

  1. Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic

    Jaroslava Cieslarová, Pavel Hanáček & Stanislav Procházka

  2. Department of Plant Biotechnology, AGRITEC Plant Research Ltd., Zemědělská 2520/16, 787 01, Šumperk, Czech Republic

    Petr Smýkal & Miroslav Griga

  3. Department of Biology, University of Ostrava, Chittusiho 10, Ostrava, Czech Republic

    Zuzana Dočkalová

  4. Grain Legumes Department, AGRITEC Plant Research Ltd., Zemědělská 2520/16, 787 01, Šumperk, Czech Republic

    Miroslav Hýbl

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  1. Jaroslava Cieslarová
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Correspondence to Petr Smýkal.

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Jaroslava Cieslarová and Petr Smýkal authors contributed equally to the work.

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Cieslarová, J., Smýkal, P., Dočkalová, Z. et al. Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance. Genet Resour Crop Evol 58, 439–451 (2011). https://doi.org/10.1007/s10722-010-9591-3

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