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Genetic diversity within Pisum sativum using protein- and PCR-based markers

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Abstract

A collection of 148 Pisum accessions, mostly from Western Europe, and including both primitive germplasm and cultivated types, was structured using 121 protein- and PCR-based markers. This molecular marker-based classification allowed us to trace back major lineages of pea breeding in Western Europe over the last decades, and to follow the main breeding objectives: increase of seed weight, introduction of the afila foliage type and white flowers, and improvement of frost tolerance for winter-sown peas. The classification was largely consistent with the available pedigree data, and clearly resolved the different main varietal types according to their end-uses (fodder, food and feed peas) from exotic types and wild forms. Fodder types were further separated into two sub-groups. Feed peas, corresponding to either spring-sown or winter-sown types, were also separated, with two apparently different gene pools for winter-sown peas. The garden pea group was the most difficult to structure, probably due to a continuum in breeding of feed peas from garden types. The classification also stressed the paradox between the narrowness of the genetic basis of recent cultivars and the very large diversity available within P. sativum. A sub-collection of 43 accessions representing 96% of the whole allelic variability is proposed as a starting point for the construction of a core collection.

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Acknowledgements

The authors thank M. Ambrose (John Innes Centre, Norwich), S. Anguelova (International Programme for Genetic Resources, Plovdiv), A. Burghoffer (Institut National pour la Recherche Agronomique, Versailles), C. Coyne (United States Department of Agriculture, Pullman), A. Ramos Monreal (University of Valladolid), M. Vishnyakova (NI Vavilov Research Institute of Plant Industry, St Petersburg), and X. Zong (Chinese Academy of Agricultural Sciences, Beijing) for providing accessions with related information. They also thank M. Duparque, P. Marget and G. Morin for the description of the accessions, T. Bataillon for his help with the MSTRAT analysis, P. Dubreuil, M. Manzanares, M.L. Pilet-Nayel, R. Thompson and M. Trottet for helpful comments on the manuscript. Finally, we are very grateful to R. Cousin for spending time in tracing back the history of pea breeding with us, and to M. Chauvet for sharing his knowledge on the history of pea and its uses.

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Authors and Affiliations

  1. Domaine de la Motte, UMR INRA-ENSAR APBV, BP35327, 35653, Le Rheu, France

    A. Baranger & G. Deniot

  2. INRA URGAP BV1540, 21034, Dijon, Cedex, France

    G. Aubert, J. Potier, C. Weinachter & J. Burstin

  3. Domaine Brunehaut, INRA GAP, 80200, Peronne, France

    A. L. Lainé & I. Lejeune-Hénaut

  4. Domaine du Magneraud, GEVES SEV, Saint Pierre d’Amilly, BP52, 17700, Surgères, France

    G. Arnau & J. Lallemand

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  1. A. Baranger
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  2. G. Aubert
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  3. G. Arnau
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  4. A. L. Lainé
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  5. G. Deniot
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  8. I. Lejeune-Hénaut
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  9. J. Lallemand
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  10. J. Burstin
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Correspondence to A. Baranger.

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Communicated by C. Möllers

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Baranger, A., Aubert, G., Arnau, G. et al. Genetic diversity within Pisum sativum using protein- and PCR-based markers. Theor Appl Genet 108, 1309–1321 (2004). https://doi.org/10.1007/s00122-003-1540-5

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  • DOI: https://doi.org/10.1007/s00122-003-1540-5

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