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Allelic variations of α-gliadin genes from species of Aegilops section Sitopsis and insights into evolution of α-gliadin multigene family among Triticum and Aegilops

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Abstract

The α-gliadins account for 15–30 % of the total storage protein in wheat endosperm and play important roles in the dough extensibility and nutritional quality. On the other side, they act as a main source of toxic peptides triggering celiac disease. In this study, 37 α-gliadins were isolated from three species of Aegilops section Sitopsis. Sequence similarity and phylogenetic analyses revealed novel allelic variation at Gli-2 loci of species of Sitopsis and regular organization of motifs in their repetitive domain. Based on the comprehensive analyses of a large number of known sequences of bread wheat and its diploid genome progenitors, the distributions of four T cell epitopes and length variations of two polyglutamine domains are analyzed. Additionally, according to the organization of repeat motifs, we classified the α-gliadins of Triticum and Aegilops into eight types. Their most recent common ancestor and putative divergence patterns were further considered. This study provides new insights into the allelic variations of α-gliadins in Aegilops section Sitopsis, as well as evolution of α-gliadin multigene family among Triticum and Aegilops species.

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Authors and Affiliations

  1. College of Landscape and Architecture, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Zhuo Huang

  2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, Sichuan, China

    Hai Long

  3. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Yu-Ming Wei, Ze-Hong Yan & You-Liang Zheng

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  1. Zhuo Huang
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  2. Hai Long
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  3. Yu-Ming Wei
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  4. Ze-Hong Yan
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  5. You-Liang Zheng
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Correspondence to Hai Long or You-Liang Zheng.

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Huang, Z., Long, H., Wei, YM. et al. Allelic variations of α-gliadin genes from species of Aegilops section Sitopsis and insights into evolution of α-gliadin multigene family among Triticum and Aegilops . Genetica 144, 213–222 (2016). https://doi.org/10.1007/s10709-016-9891-4

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  • DOI: https://doi.org/10.1007/s10709-016-9891-4

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