Abstract
Glutenin and gliadin subunits play a key role in flour processing quality by network formation in dough. Wild relatives of crops have served as a pool of genetic variation for decades. In this study, 180 accessions from 12 domesticated and wild relatives of wheat were characterized for the glutenin and gliadin genes with allele-specific molecular markers. A total of 24 alleles were detected for the Glu-A3 and Gli-2A loci, which out of 19 amplified products identified as new alleles. Analysis of molecular variance (AMOVA) indicated that 90 and 65% of the genetic diversity were partitioned within two Aegilops and Triticum genera and their species, respectively. Furthermore, all glutenin and gliadin analyzed loci were polymorphic, indicating large genetic diversity within and between the wild species. Our results revealed that allelic variation of Glu-3A and Gli-As.2 is linked to genomic constitutions so that, Ae. caudata (C genome), Ae. neglecta (UM genome), Ae. umbellulata (U genome) and T. urartu (Au genome) harbor wide variation in the studied subunits. Hence, these species can be used in wheat quality breeding programs.
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Aguiriano, E., Ruiz, M., Fite, R., Carrillo, J.M. 2006. Analysis of genetic variability in a sample of the durum wheat (Triticum durum Desf.) Spanish collection based on gliadin markers. Genet. Resour. Crop. Evol. 53:1543–1552.
Ahmadi, J., Pour-Aboughadareh, A. 2015. Allelic variation of glutenin and gliadin genes in Iranian einkorn wheat. Bio Env Sci. 7:168–179.
Ahmadpoor, F., Asghari-Zakaria, R., Firoozi, B., Shahbazi, H. 2014. Investigation of diversity in Aegilops biuncialis and Aegilops umbellulata by A-PAGE. Nat. Prod. Res. 28:1626–1636.
An, X., Zhang, Q., Yan, Y., Li, Q., Zhang, Y., Wang, A., Pei, Y., Tian, J., Wang, H., Hsam, S.L.K., Zeller, F.J. 2006. Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theor. Appl. Genet. 113:383–395.
Caballero, L., Martin, M.A., Alvarez, J.B. 2008. Allelic variation for the high- and low-molecular-weight glutenin subunits in wild diploid wheat (Triticum urartu) and its comparison with durum wheats. Aust. J. Agric. Res. 59:906–910.
Cuesta, S., Alvarez, J.B., Guzman, C. 2017. Identification and molecular characterization of novel LMW-m and –s glutenin genes, and a chimeric–m/-I glutenin gene in 1A chromosome of three diploid Triticum species. J. Cereal. Sci. 74:46–55.
Doyle, J.J., Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical. Bulletin. 19:11–15.
Huang, Z., Long, H., Wei, Y.M., Yan, Z.H., Zheng, Y.L. 2016. Allelic variations of α-gliadin genes from species of Aegilops section Sitopsis and insights into evolution of α-gliadin multigene family among Triticum and Aegilops. Genetica. 144:213–222.
Jiang, C., Pei, Y., Zhang, Y., Li, X., Yao, D., Yan, Y., Ma, W., Hsam, S.L.K., Zeller, F.J. 2008. Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi. Hereditas. 145:92–98.
Jin, H., Yan, J., Pena, R.J., Xia, X.C., Morgounov, A., Han, L.M., Zhang, Y., He, Z.H. 2011. Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. Crop. Pasture. Sci. 62:746–754.
Kawaura, K., Mochida, K., Ogihara, Y. 2005. Expression profile of two storage-protein gene families in hexaploid wheat revealed by large-scale analysis of expressed sequence tags. Plant. Physiol. 139:1870–1880.
Kimber, G., Feldman, M. 1987. Wild wheat. An introduction. Special Report No. 353, Missouri, College of Agriculture, University of Missouri-Columbia.
Lee, J.Y., Beom, H.R., Altenbach, S.B., Lim, S.H., Kim, Y.T., Kang, C.S., Yoon, U.H., Gupta, R., Kim, S.T., Ahn, S.N., Kim, Y.M. 2016. Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Funct. Integr. Genomics. 16:269–279.
Li, J., Wang, S.L., Cao, M., Lv, D.W., Subburaj, S., Li, X.H., Zeller, F.J., Hsam, S.L.K., Yan, Y.M. 2013. Cloning, expression, and evolutionary analysis of a-gliadin genes from and genomes. J. Appl. Genet. 54:157–167.
Long, H., Wei, Y., Yan, Z.H., Baum, B., Nevo, E. 2005. Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant. Biol. 15:322
Luo, G., Zhang, X., Zhang, Y., Yang, W., Li, Y., Sun, J., Zhan, K., Zhang, A., Liu, D. 2015. Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant. Biol. 15:322
Ma, Z.C., Wei, Y.M., Long, H., Yan, Z.H., Baum, B., Zheng, Y.L. 2006. Characterization of low-molecular-weight i-type glutenin subunit genes from diploid wheat in relation to the gene family structure. Mol. Biol. 40:897–906.
Payne, I.P., Jackson, E.A., Holt, L.M., Law, C.N. 1984. Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theor. Appl. Genet. 67:235–243.
Payne, P.I., Nightingale, M.A., Krattiger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food. Agr. 40:51–65.
Peakall, R., Smouse, P.E. 2006. GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol. Ecol. Notes. 6:288–295.
Pour-Aboughadareh, A., Ahmadi, J., Mehrabi, A.A., Etminan, A., Moghaddam, M., Siddique, K.H.M. 2017a. Physiological responses to drought stress in wild relatives of wheat: implications for wheat improvement. Acta. Physiol. Plant. 39:106
Pour-Aboughadareh, A., Ahmadi, J., Mehrabi, A.A., Moghaddam, M., Etminan, A. 2017b. Evaluation of agromorphological diversity in wild relatives of wheat collected in Iran. J. Agr. Sci. Tech. 19:943–956.
Pour-Aboughadareh, A., Mahmoudi, M., Moghaddam, M., Ahmadi, J., Mehrabi, A.A., Alavikia, S.S. 2017c. Agro-morphological and molecular variability in Triticum boeoticum accessions from Zagros Mountains, Iran. Genet. Resour. Crop. Evol. 64:545–556.
Van Slageren, M.W. 1994. Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. and Spach) Eig (Poaceae). Wageningen Agricultural University Papers. Wageningen, The Netherlands.
Wan, Y., Wang, D., Shewry, P.R., Halford, N.G. 2002. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor. Appl. Genet. 104:828–839.
Wang, L., Li, G., Penea, R.J., Xia, X., He, Z. 2010. Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). J. Cereal. Sci. 51:305–312.
Wang, L.H., Zhao, X.L., He, Z.H., Ma, W., Appels, R., Peña, R.J., Xia, X.C. 2009. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor. Appl. Genet. 118:525–539.
Xu, S.S., Khan, K., Klindworth, D.L., Nygard, G. 2010. Evaluation and characterization of high-molecular weight 1D glutenin subunits from Aegilops tauschii in synthetic hexaploid wheats. J. Cereal. Sci. 52:333–336.
Zhang, X., Liu, D., Zhang, J., Jiang, W., Luo, G., Yang, W., Sun, J., Tong, Y., Cui, D., Zhang, A. 2013. Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. J. Exp. Bot. 64:2027–2040.
Zhang, X.F., Jin, H., Zhang, Y., Liu, D.C., Li, G.Y., Xia, X.C., He, Z.H., Zhang, A.M. 2012. Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biol. 12:243
Zhang, Y., Luo, G., Liu, D., Wang, D., Yang, W., Sun, J., Zhang, A., Zhan, K. 2015. Genome, transcriptome-and proteome-wide analyses of the gliadin gene families in Triticumurartu. PLoS One. 10:e0131559.
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Communicate by M. Molnár-Láng
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Ahmadi, J., Pour-Aboughadareh, A., Fabriki-Ourang, S. et al. Molecular Detection of Glutenin and Gliadin Genes in the Domesticated and Wild Relatives of Wheat using Allele-specific Markers. CEREAL RESEARCH COMMUNICATIONS 46, 510–520 (2018). https://doi.org/10.1556/0806.46.2018.039
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DOI: https://doi.org/10.1556/0806.46.2018.039