Abstract
Wild rice is a valuable resource for the genetic improvement of cultivated rice (Oryza sativa L., AA genome). Molecular markers are important tools for monitoring gene introgression from wild rice into cultivated rice. In this study, Simple sequence repeat (SSR) markers were used to analyze interspecific hybrids of O. sativa–O. officinalis (CC genome), the backcrossing progenies and the parent plants. Results showed that most of the SSR primers (335 out of 396, 84.6%) developed in cultivated rice successfully amplified products from DNA samples of wild rice O. officinalis. The polymorphism ratio of SSR bands between O. sativa and O. officinalis was as high as 93.9%, indicating differences between the two species with respect to SSRs. When the SSR markers were applied in the interspecific hybrids, only a portion of SSR primers amplified O. officinalis-specific bands in the F1 hybrid (52.5%), BC1 (52.5%), and MAALs (37.0%); a number of the bands disappeared. Of the 124 SSR loci that detected officinalis-specific bands in MAAL plants, 96 (77.4%) showed synteny between the A and C-genomes, and 20 (16.1%) showed duplication in the C-genome. Sequencing analysis revealed that indels, substitution and duplication contribute to the diversity of SSR loci between the genomes of O. sativa and O. officinalis.
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This study was supported by grants from the National Program of High Technology Development and the National Special Program for Research and Industrialization of Transgenic Plants of China.
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Li, G., Hu, W., Qin, R. et al. Simple sequence repeat analyses of interspecific hybrids and MAALs of Oryza officinalis and Oryza sativa . Genetica 134, 169–180 (2008). https://doi.org/10.1007/s10709-007-9222-x
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DOI: https://doi.org/10.1007/s10709-007-9222-x