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Marker assisted transfer of anthracnose resistance gene Co-5 to elite French bean (Phaseolus vulgaris L.) background

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Abstract

Anthracnose caused by Colletotrichum lindemuthianum is one of the most prevalent and severe disease of beans. Anthracnose resistance to most of the prevalent races in the state is provided by Co-5 gene. Therefore, deployment of this gene is more relevant as the lines developed under this study harboring Co-5 gene have potential to impart resistance against prevalent races. Hybridisation between a resistant genotype, TU having bean anthracnose resistance gene Co-5 and elite cultivar Arka Komal was done and further advanced to generate F1, BC1F1, BC2F1 and BC2F2 plants. Co-5 gene linked SCAR marker SAB3 was used for screening of hybridity of F1, BC1F1, BC2F1 and BC2F2 plants/pods. The results of the foreground selection in these plants revealed that out of a population of 50 BC2F2 plants, 36 plants portrayed Co-5 gene. Further foreground selection with SAB3 SCAR marker, revealed lack of segregation in 12 progenies. Screening of these progenies by detached pod and germinated seed dip methods using race 3 of C. lindemuthianum, validated Co-5 resistance in all 12 BC2F3 progenies. All 12 Co-5 gene-positive BC2F4 progenies were evaluated for 17 agronomic traits including 8 qualitative and 9 quantitative traits. Evaluation identified AKTU 19 and AKTU 33 lines that possessed better elite background. These progenies can further be advanced to isolate variety/(ies) directly or as prebred donor of anthracnose resistance gene Co-5 in the future breeding programmes.

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References

  • Alzate-Marin AL, Baia GS, de Paula TJ, de Carvalho GA, de Barros EG, Moreira MA (1997) Inheritance of anthracnose resistance in common bean differential cultivar AB136. Plant Dis 81:996–998

    Article  PubMed  Google Scholar 

  • Alzate-Marin AL, Arruda KM, de Barros EG, Moreira MA (2003a) Allelism studies for anthracnose resistance genes of common bean cultivar AND 277. Annu Rep Bean Improv Coop 46:173–174

    Google Scholar 

  • Alzate-Marin AL, Costa MR, Arruda KM, de Barros EG, Moreira MA (2003b) Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar. Euphytica 133:165–169

    Article  CAS  Google Scholar 

  • Alzate-Marin AL, de Souza KA, de Silva MG, de Oliveira EJ, Moreira MA, de Barros EG (2007) Genetic characterization of anthracnose resistance genes Co-43 and Co-9 in common bean cultivar tlalnepantla 64 (PI 207262). Euphytica 154(1/2):1–8

    Article  CAS  Google Scholar 

  • Balardin RS, Jarosz AM, Kelly JD (1997) Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central and North America. Phytopathol 87:1184–1191

    Article  CAS  Google Scholar 

  • Bannerot H (1965) Results de l’infectiond’une collection de haricots par six races physiologiquesd’anthracnose. Ann De Amélior Desplantes (Paris) 15:201–222

    Google Scholar 

  • Bansuli, Kapila RK (2022) Introgression of anthracnose resistance Co-4 gene in elite French bean (Phaseolus vulgaris L.) background using linked molecular marker SY20. Euphytica 218: 116. https://doi.org/10.1007/s10681-022-03067-z

  • Campa A, Giraldez R, Ferreira JJ (2009) Genetic dissection of the resistance to nine anthracnose races in the common bean differential cultivars MDRK and TU. Theor Appl Genet 119(1):1–11

    Article  CAS  PubMed  Google Scholar 

  • Coimbra-Goncalves GK, Goncalves-Vidigal MC, Coelho RT, Valentini G, Filho PSV, Lacanallo GF, Sousa LL, Elias HT (2016) Characterization and mapping of anthracnose resistance gene in mesoamerican common bean cultivar crioulo 159. Crop Sci 56:2904–2915

    Article  CAS  Google Scholar 

  • Debouck DG, Smartt J (1995) Beans Phaseolus spp. (Leguminosae Papilionatae). In: Simmonds NW, Smartt J (eds) Evolution of crop plants, 2nd edn. Longman, London, pp 287–294

    Google Scholar 

  • Drijfhout E, Davis JHC (1989) Selection of a new set of a homogenously reacting beam differentials to differentiate races of Colletotrichum lindemuthianum. Plant Pathol 38:391–396

    Article  Google Scholar 

  • FAO (Food and Agriculture Organization) (2020) www.faostat.fao.org (5th March 2023)

  • Fouilloux G (1976) Bean anthracnose: New genes of resistance. Annu Rpt Bean Improv Coop 19:36–37

    Google Scholar 

  • Fouilloux G (1979) New races of bean anthracnose and consequences in our breeding programs. In: Proceedings of the International Symposium of Diseases of Tropical Food Crops (H. Maraite and J.A. Meyer, editors) Louvain-la-Neuve, Belgium. 4–8 Sept. 1978. UniversitéCatholique de Louvain, Louvain-la-Neuve, Belgium, pp. 221–235

  • Frias AAT, Gonçalves-Vidigal MC, Nanami DSY, Castro SAL, Vidigal Filho PS, Lacanallo GF (2016) Genetic resistance to Colletotrichum lindemuthianumin the Andean cultivar JaloPintado 2 of common bean. Agron Sci Biotechnol 2(1):21–28

    Article  Google Scholar 

  • Garzon LN, Ligarreto GA, Blair MW (2008) Molecular marker-assisted backcrossing of anthracnose resistance into Andean climbing beans (Phaseolus vulgaris L.). Crop Sci 48(2):562–570

    Article  CAS  Google Scholar 

  • Geffroy V, Sicard D, de Oliveira JCF, Sevignac M, Cohen S, Gepts P, Neema C, Langin T, Dron M (1999) Identification of an ancestral resistance gene cluster involved in the co-evolution process between Phaseolus vulgaris and its fungal pathogen Colletotrichum lindemuthianum. Mol Plant-Microbe Interact 12:774–784

    Article  CAS  PubMed  Google Scholar 

  • Geffroy V, Sevignac M, Billant P, Dron M, Langin T (2008) Resistance to Colletotrichum lindemuthianum in Phaseolus vulgaris: a case study for mapping two independent genes. Theor Appl Genet 116(3):407–415

    Article  CAS  PubMed  Google Scholar 

  • Geffroy V (1997) Dissection genetique de la resistance a Colletotrichum lindemuthianum, agent de l’anthracnose, chez deuxgenotypesrepresentatifs des pools geniques de Phaseolus vulgaris. (In French.) Ph.D. diss., Inst. Natl. Agron., Paris-Grignon.

  • Goncalves-Vidigal MC, Kelly JD (2006) Inheritance of anthracnose resistance in the common bean cultivar Widusa. Euphytica 151(3):411–419

    Article  CAS  Google Scholar 

  • Goncalves-Vidigal MC, Silva CR, VidigalFilho PS, Gonela A, Kvitschal MV (2007) Allelic relationships of anthracnose (Colletotrichum lindemuthianum) resistance in the common bean (Phaseolus vulgaris L.) cultivar Michelite and the proposal of a new anthracnose resistance gene, Co-11. Genet Mol Biol 30:589–593

    Article  CAS  Google Scholar 

  • Goncalves-Vidigal MC, Lacanallo GF, VidigalFilho PS (2008) A new gene conferring resistance to anthracnose in Andean common bean (Phaseolus vulgaris L.) cultivar “JaloVermelho.” Plant Breed 127(6):592–596

    Article  Google Scholar 

  • Goncalves-Vidigal MC, VidigalFilho PS, Medeiros AF, Pastor-Corrales MA (2009) Common bean landrace JaloListrasPretas is the source of a new Andean anthracnose resistance gene. Crop Sci 49(1):133–138

    Article  CAS  Google Scholar 

  • Goncalves-Vidigal MC, Meirelles AC, Poletine JP, Sousa LL, Cruz AS, Nunes MP, Lacanallo GF, Filho PSV (2012) Genetic analysis of anthracnose resistance in Pitanga dry bean cultivar. Plant Breed 131:423–429. https://doi.org/10.1111/j.1439-0523.2011.01939.x

    Article  CAS  Google Scholar 

  • Goncalves-Vidigal MC, Cruz AS, Lacanallo GF et al (2013) Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro. Theor Appl Genet 126:2245–2255. https://doi.org/10.1007/s00122-013-2131-8

    Article  CAS  PubMed  Google Scholar 

  • Gu Yu, Han Q, Wang W, Li S, Sun D, Wu F (2011) Application of common bean anthracnose resistance gene SCAR markers in snap bean disease resistance identification. Acta Hortic Sin 38(5):911–920

    CAS  Google Scholar 

  • Hospital F (2005) Selection in backcross programmes. Philos Trans R Soc B 360:1503–1511

    Article  CAS  Google Scholar 

  • Kamiri AK, Arunga EE, Rotich F, Otsyula R (2021) Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers. Afr J Biotechnol 20(2):51–65

    Article  CAS  Google Scholar 

  • Kaur S, Narpinder S, Navdeep SS, Rana JC (2009) Diversity in properties of seed and flour of kidney bean germplasm. Food Chem 117(2):282–289

    Article  CAS  Google Scholar 

  • Kelly JD, Young RA (1996) Proposed symbols for anthracnose resistance genes. Annu Rep Bean Improv Coop 39:20–24

    Google Scholar 

  • Khetan S, Singh N, Kaur A, Rana JC (2015) Structural and functional characterization of kidney bean and field pea protein isolates: a comparative study. Food Hydrocoll 43:679–689

    Article  Google Scholar 

  • Kwak M, Gepts P (2009) Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L. Fabaceae). Theor Appl Genet 118:979–992

    Article  CAS  PubMed  Google Scholar 

  • Lateef I, Katoch S, Katoch A et al (2024) Fine mapping of a new common bean anthracnose resistance gene (Co-18) to the proximal end of Pv10 in Indian landrace KRC-5. Theor Appl Genet 137:32. https://doi.org/10.1007/s00122-023-04539-z

    Article  CAS  PubMed  Google Scholar 

  • Limongelli G, Laghetti G, Perrino P, Piergiovanni AR (1996) Variation of seed storage proteins in landraces of common bean (Phaseolus vulgaris L.) from Basilicata, southern Italy. Euphytica 92:393–399

    Article  CAS  Google Scholar 

  • Manjula V, Shyamalamma S, Hosamani A, Geetha MS (2016) Validation of SCAR markers linked to anthracnose resistant in French bean (Phaseolus vulgaris L.) germplasm. Green Farm 7(2):379–382

    Google Scholar 

  • Mastenbroek C (1960) A breeding programme for resistance to anthracnose in dry shell haricot beans, based on a new gene. Euphytica 9:177–184

    Article  Google Scholar 

  • Mayee CD, Dattar VV (1986) Phytopathometry. Technical Bulletin-1. Marathwada Agricultural University, Parbhani. pp 88–89

  • McRostie GP (1919) Inheritance of anthracnose resistance as indicated by a cross between a resistant and a susceptible bean. Phytopathology 9:141–148

    Google Scholar 

  • Melotto M, Kelly JD (2000) An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica 116:143–149

    Article  Google Scholar 

  • Mendez-Vigo B, Rodriguez-Suarez C, Paneda A, Ferreira JJ, Giraldez R (2005) Molecular markers and allelic relationships of anthracnose resistance gene cluster B4 in common bean. Euphytica 141:237–245

    Article  CAS  Google Scholar 

  • Mumba LE, Galway NW (1998) Compatibility of crosses between gene pools and evolutionary classes in the common bean (Phaseolus vulgaris L.). Genet Resour Crop Evol 45:69–80

    Article  Google Scholar 

  • Murray HG, Thompson WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4325

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nunes MPBA, Goncalves-Vidigal MC, Martins VSR, Xavier LFS, Valentini G, Bisneta MV, Filho PSV (2021) Relationship of Colletotrichum lindemuthianum races and resistance loci in the Phaseolus vulgaris L. genome. Crop Sci 61:3877–3893

    Article  CAS  Google Scholar 

  • Pachico D (1993) The demand for bean technology: In G. Henry (ed) Trends in CIAT’s commodities, CIAT Working Document no. 128: 60–73, Cali, Colombia

  • Panse VG, Sukhatme PV (1984) Statistical methods for agricultural workers. ICAR, New Delhi

    Google Scholar 

  • Pastor-Corrales MA, Tu JC (1989) Anthracnose. In: Schwartz HF, Pastor-Corrales MA (eds) Bean production problems in the tropics. CIAT, Cali, pp 77–104

    Google Scholar 

  • Pastor-Corrales MA, Erazo OA, Estrada EI, Singh SP (1994) Inheritance of anthracnose resistance in common bean accession G 2333. Plant Dis 78:959–962. https://doi.org/10.1094/PD-78-0959

    Article  Google Scholar 

  • Pastor-Corrales MA, Otoya MM, Molina A, Singh SP (1995) Resistance to Colletotrichum lindemuthianum isolates from Middle America and Andean South America in different common bean races. Plant Dis 79:63–67

    Article  Google Scholar 

  • Pathania A, Sharma PN, Sharma OP, Chahhota RK, Padder BA, Sharma P (2006) Evaluation of resistance sources and inheritance of resistance in kidney bean to Indian virulences of Colletotrichum lindemuthianum - evaluation of resistance in bean to anthracnose. Euphytica 149(1/2):97–103

    Article  Google Scholar 

  • Peloso MJ Del (1992) Antracnose do feijoeiro no Estado de Minas Gerais-Brasil. In: Pastor-Corrales M (Ed) La antracnosis del frijol comun, Phaseolusvulgaris, en America Latina. Doc. de Trabajo No. 113. CIAT, Cali, Colombia, pp 86–108

  • Rodriguez-Suarez C, Ferreira JJ, Campa A, Paneda A, Giraldez R (2008) Molecular mapping and intra-cluster recombination between anthracnose race-specific resistance genes in the common bean differential cultivars Mexico 222 and Widusa. Theor Appl Genet 116(6):807–814

    Article  CAS  PubMed  Google Scholar 

  • Schwartz HF, Pastor-Corrales MA, Singh SP (1982) New sources of resistance to anthracnose and angular leaf spot of beans (Phaseolus vulgaris). Euphytica 31:741–754

    Article  Google Scholar 

  • Sharma PN, Kumar A, Sharma OP, Sud D, Tyagi PD (1999) Pathogenic variability in Colletotrichum lindemuthianum and evaluation of resistance in Phaseolus vulgaris in the north western Himalayan region of India. J Phtytopathol 147:41–45

    Google Scholar 

  • Sharma PN, Padder BA, Sharma OP, Pathania A, Sharma P (2007) Pathological and molecular diversity in Colletotrichum lindemuthianum (bean anthracnose) across Himachal Pradesh, a North-Western Himalayan state of India. Australas Plant Pathol 36(2):191–197

    Article  CAS  Google Scholar 

  • Sharma N, Kumari N, Sharma SK, Padder BA, Sharma PN (2019) Investigating the virulence and genetic diversity of Colletotrichum lindemuthianum populations distributed in the North Western Himalayan hill states. J Plant Pathol. https://doi.org/10.1007/s42161-019-00269-8

    Article  Google Scholar 

  • Sharma PN, Sharma OP, Sharma P and Pathania A (2003) RAPD analysis of diverse isolates of Colletotrichum lindemuthianum infecting kidney bean in Himachal Pradesh. In: Symposium on Challenging Problems in Horticultural and Forest Pathology, 14–15 Nov., 2003, UHF, Solan, p 26

  • Smartt J (1990) Grain legumes. Evolution and genetic resources. Cambridge University Press, Cambridge, p 379

    Book  Google Scholar 

  • Sousa LL, Cruz AS, VidigalFilho PS, Vallejo VA, Kelly JD, Goncalves-Vidigal MC (2014) Genetic mapping of the resistance allele Co-52 to Colletotrichum lindemuthianum in the common bean MSU 7–1 line. Aust J Crop Sci 8(2):317–323

    Google Scholar 

  • Sousa LL, Gonçalves AO, Gonçalves-Vidigal MC, Lacanallo GF, Fernandez AC, Awale H, Kelly JD (2015) Genetic Characterization and Mapping of Anthracnose Resistance of Common Bean Landrace Cultivar Corinthiano. Crop Sci 55:1900–1910

    Article  CAS  Google Scholar 

  • Trabanco N, Campa A, Ferreira JJ (2015) Identification of a new chromosomal region involved in the genetic control of resistance to anthracnose in common bean. Plant Genome 8:1–11

    Article  CAS  Google Scholar 

  • Vallejo V, Kelly JD (2001) Development of a SCAR marker linked to the Co5 locus in common bean. Annu Rep Bean Improv Coop 44:21–122

    Google Scholar 

  • Young RA, Kelly JD (1996) Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. Plant Dis 80:650–654

    Article  Google Scholar 

  • Young RA, Kelly JD (1997) RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Sci 37:940–946

    Article  CAS  Google Scholar 

  • Young RA, Melotto M, Nodari RO, Kelly JD (1998) Marker-assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar, “G2333.” Theor Appl Genet 96(1):87–94

    Article  CAS  Google Scholar 

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Acknowledgements

The first author is thankful to Department of Agricultural Biotechnology, CSK HPKV, Palampur, Himachal Pradesh, India for providing facilities in the department for conduct of the research.

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We want to acknowledge the Department of Agricultural Biotechnology, CSKHPKV, Palampur, Himachal Pradesh, India for providing funds and all the facilities for conducting the research.

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  1. Department of Agricultural Biotechnology, CSK HPKV, Palampur, Himachal Pradesh, 176062, India

    Bansuli

  2. Department of Seed Science & Technology, CSK HPKV, Palampur, Himachal Pradesh, India, 176062

    Rakesh Kumar Kapila

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Bansuli conducted the experiments, analyzed the data and wore the final manuscript. RK Kapila conceptualized the research programme and helped in main manuscript writing and reviewing the manuscript.

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Bansuli, Kapila, R.K. Marker assisted transfer of anthracnose resistance gene Co-5 to elite French bean (Phaseolus vulgaris L.) background. Euphytica 220, 71 (2024). https://doi.org/10.1007/s10681-024-03321-6

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