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Genetic mapping and development of near-isogenic lines for genes governing a liguleless phenotype in tetraploid wheat

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Abstract

The liguleless phenotype in wheat lacks ligules and auricles on all plant leaves. Two duplicate dominant genes (Lg 1 and Lg 3 ) principally control the presence of ligules in tetraploid wheat. Although the Lg 3 gene was tentatively located in chromosome 2A, a genetic stock of Triticum durum Desf. (2n = 4x = 28, BBAA genome) having genotype lg 1 lg 1 Lg 3 Lg 3 has not been available. We speculated that a wild emmer (T. dicoccoides Körn., 2n = 4x = 28, BBAA genome) could be a possible source of the Lg 3 gene. In the present study near-isogenic lines (NIL’s) ANW 12E for genotype lg 1 lg 1 Lg 3 Lg 3 and ANW 12F for genotype Lg 1 Lg 1 lg 3 lg 3 were developed from a cross between ANW 12A (lg 1 lg 1 lg 3 lg 3 ), a liguleless NIL of T. durum LD222, and LDN(DIC 2A) (Lg 1 Lg 1 Lg 3 Lg 3 ) through backcrossing to ANW 12A. LDN(DIC 2A) is a chromosome substitution line in which a pair of T. durum cv. Langdon (LDN) 2A chromosomes is replaced with a pair of chromosomes 2A from T. dicoccoides (DIC) line Israel A. Molecular mapping using the F2 of liguleless durum PI 370751/ANW 12F revealed that the Lg 1 gene was 17.5 cM distal to Xgwm47 in chromosome 2BL. In the F2 of PI 370751/ANW 12E the Lg 3 gene was located 18.6 cM distal to Xgwm312 in chromosome 2AL. These results confirmed that the Lg 3 locus was present in chromosome 2A, and dominant alleles at the homoeologous Lg loci determine the presence of ligules in polyploid wheat.

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Acknowledgments

We acknowledge gifts of seed from the National Small Grain Collection (NGSC), Aberdeen, Idaho and USDA-ARS, Fargo, North Dakota, USA. We thank Dr. D. L. Klindworth, USDA-ARS, Northern Crop Science Laboratory, Fargo for helpful comments on the manuscript.

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Authors and Affiliations

  1. College of Agriculture, Ibaraki University, 3-21-1 Chuo, Ami, Inashiki, Ibaraki, 300-0393, Japan

    Y. Amagai, N. Watanabe & T. Kuboyama

  2. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, 180-8509, Japan

    Y. Amagai

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  1. Y. Amagai
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  2. N. Watanabe
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  3. T. Kuboyama
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Correspondence to N. Watanabe.

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Amagai, Y., Watanabe, N. & Kuboyama, T. Genetic mapping and development of near-isogenic lines for genes governing a liguleless phenotype in tetraploid wheat. Euphytica 205, 851–857 (2015). https://doi.org/10.1007/s10681-015-1421-4

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