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Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding

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Abstract

Rice leaf folder, stem borer and brown planthopper (BPH) are the most devastating rice insect pests. Developing and planting insect-resistant rice varieties is the most economical and effective measure for controlling these pests. BPH can be controlled with native BPH-resistance genes in rice, while at present rice leaf folder and stem borer can only be controlled through planting transgenic Bt rice. In this study, the breeding of a new restorer line KR022 possessing stacked BPH-resistance genes Bph14 and Bph15, Bt gene cry1C and glufosinate-resistance gene bar, is reported for the first time. A rice restorer line R022 with BPH-resistance genes Bph14 and Bph15 was used as a recurrent parent to cross with the transgenic rice T1C-19 of cry1C and bar genes during the breeding process. The restorer line KR022 was developed from the backcross populations of R022 and T1C-19 through molecular marker-assisted selection and glufosinate-resistance selection. The cry1C and bar genes were found to integrate on chromosome 11 of KR022, and the genome recovery of KR022 was up to 95.8 % of the R022 genome. The quantification of Cry1C protein expression showed that it was expressed at different levels in the leaf, stem, panicle, endosperm, and root of KR022 and its hybrid rice. The insect-resistance evaluation indicated that KR022 and its hybrid rice had good resistance to rice leaf folder and stem borer, both in laboratory settings and in the field. Furthermore, they exhibited increased resistance to BPH at both the seedling and mature stage. The field trial showed there was no significant difference in key agronomic traits between KR022 and its recurrent parent R022, and four hybrids from KR022 yield much higher than the control II-You 838. Moreover, KR022 and its hybrid rice were found to have resistance to the herbicide glufosinate. These results demonstrate that KR022 is effective as a rice restorer line for the breeding of “green super rice”, possessing multiple tolerances to rice BPH, stem borer, leaf folder and glufosinate.

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Abbreviations

BPH:

Brown planthopper

Bt:

Bacillus thuringiensis

dNTP:

Deoxy-ribonucleoside triphosphate

ELISA:

Enzyme-linked immunosorbent assay

GM:

Genetically-modified

MAS:

Molecular marker-assisted selection

PCR:

Polymerase chain reaction

PPT:

Phosphinothricin

SSB:

Striped stem borer

YSB:

Yellow stem borer

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Acknowledgments

We thank Dr. Weihua Ma in the National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan, China) for the help in SSB bioassay. This work was supported by the National Program of Transgenic Variety Development of China (2011ZX08001-001) and the Hubei Centre of Innovation for Agricultural Science and Technology (China).

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Authors and Affiliations

  1. Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crop Research Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People’s Republic of China

    Bingliang Wan, Zhongping Zha, Jinbo Li, Mingyuan Xia, Xueshu Du & Desuo Yin

  2. National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yongjun Lin

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  1. Bingliang Wan
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  2. Zhongping Zha
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  3. Jinbo Li
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  4. Mingyuan Xia
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  5. Xueshu Du
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  7. Desuo Yin
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Correspondence to Bingliang Wan.

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Wan, B., Zha, Z., Li, J. et al. Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding. Euphytica 195, 129–142 (2014). https://doi.org/10.1007/s10681-013-0988-x

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