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Allele-specific amplification for the detection of ascochyta blight resistance in chickpea

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Abstract

A new co-dominant molecular marker, CaETR, was developed based on allelic sequence length polymorphism in an ethylene receptor-like gene located in the genomic region of a QTL (QTLAR1) conferring ascochyta blight resistance in chickpea. This marker not only discriminated resistance and susceptible phenotypes of chickpea to ascochyta blight, but also easily detected heterozygous genotypes. Using the CaETR marker in combination with a previously developed co-dominant SCAR marker (SCY17590) linked to another QTL (QTLAR2) it was possible to detect resistance alleles in 90 % of resistant accessions in a collection of landraces, advances breeding lines and cultivars, and also detected susceptible alleles in all cases. The results of this study offer a scope for improving the efficiency of conventional chickpea breeding by carrying out negative selection for QTLAR1 and QTLAR2 in early generations without relaying directly on the phenotype. This PCR-based approach using both co-dominant markers is proposed as an efficient tool for selecting blight-resistant genotypes in breeding programs.

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Acknowledgments

This research was partly developed at the University of Cordoba and Instituto de Investigación y Formación Agraria y Pesca (IFAPA) Córdoba, Spain, belonging to the Campus de Excelencia Internacional Agroalimentario (ceiA3), and was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN; projects RTA2010-00059 and AGL2005-07497-CO2-01/AGR.2003), co-financed by Fondo Europeo de Desarrollo Regional (FEDER).

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Authors and Affiliations

  1. Department of Genetic, Córdoba University, Campus Rabanales, Edif. C5, 14071, Córdoba, Spain

    E. Madrid, T. Millán & J. Gil

  2. Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Washington State University, Pullman, WA, 99164, USA

    W. Chen & P. N. Rajesh

  3. Trait Genetics and Technologies, Dow AgroSciences, Indianapolis, IN, 46268, USA

    P. N. Rajesh

  4. Área de Mejora y Biotecnología, IFAPA Centro “Alameda del Obispo”, Apdo 3092, 14080, Córdoba, Spain

    P. Castro

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  1. E. Madrid
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  2. W. Chen
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  3. P. N. Rajesh
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  4. P. Castro
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  5. T. Millán
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  6. J. Gil
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Correspondence to E. Madrid.

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Madrid, E., Chen, W., Rajesh, P.N. et al. Allele-specific amplification for the detection of ascochyta blight resistance in chickpea. Euphytica 189, 183–190 (2013). https://doi.org/10.1007/s10681-012-0753-6

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  • DOI: https://doi.org/10.1007/s10681-012-0753-6

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