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Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight

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Abstract

Ascochyta blight in chickpea (Cicer arietinum L.) is a devastating fungal disease caused by the necrotrophic pathogen, Ascochyta rabiei (Pass.) Lab. To elucidate the genetic mechanism of pathotype-dependent blight resistance in chickpea, F7-derived recombinant inbred lines (RILs) from the intraspecific cross of PI 359075(1) (blight susceptible) × FLIP84-92C(2) (blight resistant) were inoculated with pathotypes I and II of A. rabiei. The pattern of blight resistance in the RIL population varied depending on the pathotype of A. rabiei. Using the same RIL population, an intraspecific genetic linkage map comprising 53 sequence-tagged microsatellite site markers was constructed. A quantitative trait locus (QTL) for resistance to pathotype II of A. rabiei and two QTLs for resistance to pathotype I were identified on linkage group (LG)4A and LG2+6, respectively. A putative single gene designated as Ar19 (or Ar21d) could explain the majority of quantitative resistance to pathotype I. Ar19 (or Ar21d) appeared to be required for resistance to both pathotypes of A. rabiei, and the additional QTL on LG4A conferred resistance to pathotype II of A. rabiei. Further molecular genetic approach is needed to identify individual qualitative blight resistance genes and their interaction for pathotype-dependent blight resistance in chickpea.

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References

  • Ahmad GD, Hafiz A, Ashraf M (1952) Some studies on chickpea crosses. In: Proceedings of the 4th Pakistan science conference, Peshwar, 1952. Part IV. Scientific land utilization. Pakistan Association for the Advancement of Science, Lahore, pp 15–16

  • Chen W, Muehlbauer FJ (2002) A mini-dome technique for virulence assay of Ascochyta rabiei on chickpea. In: Proceedings of 4th Canadian pulse research workshop, Edmonton, Alberta, 8–10 December 2002, pp 167–171

  • Cho S, Kumar J, Shultz JL, Anupama K, Tefera F, Muehlbauer FJ (2002) Mapping genes for double podding and other morphological traits in chickpea. Euphytica 128:285–292

    Article  CAS  Google Scholar 

  • Dey SK, Singh G (1993) Resistance to ascochyta blight in chickpea—genetic basis. Euphytica 68:147–153

    Google Scholar 

  • Flandez-Galvez H, Ades PK, Ford R, Pang ECK, Tayler PWJ (2003) QTL analysis for ascochyta blight resistance in an intraspecific population of chickpea (Cicer arietinum L.). Theor Appl Genet 107:1257–1265

    Article  CAS  PubMed  Google Scholar 

  • Hafiz A, Ashraf M (1953) Studies on the inheritance of resistance to Mycosphaerella blight in gram. Phytopathology 43:580–581

    Google Scholar 

  • Hervas A, Trapero-Casas JL, Jimenez-Diaz RM (1995) Induced resistance against fusarium wilt of chickpea by nonpathogenic races of Fusarium oxysporum f. sp. ciceri and nonpathogenic isolates of F. oxysporum. Plant Dis 79:1110–1116

    Google Scholar 

  • Hüttel B, Winter P, Weising H, Choumane W, Weigand F, Kahl G (1999) Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.). Genome 42:210–217

    PubMed  Google Scholar 

  • Jamil FF, Sarwar N, Sarwar M, Khan JA, Geistlinger J, Kahl G (2000) Genetic and pathogenic diversity within Ascochyta rabiei (Pass.) Lab. populations in Pakistan causing blight of chickpea (Cicer arietinum L.). Physiol Mol Plant Pathol 57:243–254

    Article  CAS  Google Scholar 

  • Kusmenoglu I (1990) Ascochyta blight of chickpea: inheritance and relationship to seed size, morphological traits, and isozyme variation. MS Thesis, Washington State University

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow E, Daly MJ, Lincoln SE, Newberg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Muehlbauer FJ (1996) Advances in the production of cool-season food legumes. Am J Altern Agric 11:71–76

    Google Scholar 

  • Nelson JC (1997) QGENE: software for maker-based genomic analysis and breeding. Mol Breed 3:239–245

    CAS  Google Scholar 

  • Roger C, Tivoli B, Huber L (1999) Effects of temperature and moisture on disease and fruit body development of Mycosphaerella pinodes on pea (Pisum sativum). Plant Pathol 48:1–9

    Article  Google Scholar 

  • Santra DK, Tekeoglu M, Ratnaparkhe M, Kaiser WJ, Muehlbauer FJ (2000) Identification and mapping of QTLs conferring resistance to ascochyta blight in chickpea. Crop Sci 40:1606–1612

    CAS  Google Scholar 

  • Singh KB, Reddy MV (1983) Inheritance of resistance to ascochyta blight in chickpea. Crop Sci 23:9–10

    Google Scholar 

  • Singh KB, Reddy MV (1996) Improving chickpea yield by incorporating resistance to ascochyta blight. Theor Appl Genet 92:509–515

    Article  Google Scholar 

  • Tekeoglu M, Santra DK, Kaiser WJ, Muehlbauer FJ (2000) Ascochyta blight resistance inheritance in three chickpea recombinant inbred line populations. Crop Sci 40:1251–1256

    Google Scholar 

  • Tekeoglu M, Rajesh PN, Muehlbauer FJ (2002) Integration of sequence tagged microsatellite sites to the chickpea genetic map. Theor Appl Genet 105:847–854

    Article  Google Scholar 

  • Tewari SK, Pandey MP (1986) Genetics of resistance to ascochyta blight in chickpea (Cicer arietinum L.). Euphytica 35:211–215

    Google Scholar 

  • Tuite J (1969) Plant phytopathological methods. Burgess, Minneapolis, p 239

  • Udupa SM, Baum M (2003) Genetic dissection of pathotype-specific resistance to ascochyta blight resistance in chickpea (Cicer arietinum L.) using microsatellite markers. Theor Appl Genet 106:1196–1202

    CAS  PubMed  Google Scholar 

  • Vir S, Grewal JS, Gupta VP (1975) Inheritance of resistance to Ascochyta rabiei in chickpea. Euphytica 24:209–211

    Google Scholar 

  • Weising, K, Nybom H, Wolff K, Meyer W (1994) DNA fingerprinting in plants and fungi. CRC, Boca Raton, p 336

  • Winter P, Pfaff T, Udupa SM, Hüttel B, Sharma PC, Sahi S, Arreguin-Espinoza R, Weigand F, Muehlbauer FJ, Kahl G (1999) Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol Gen Genet 262:90–101

    CAS  PubMed  Google Scholar 

  • Winter P, Benko-Iseppon AM, Huttel B, Ratnaparkhe M, Tullu A, Sonnante G, Pfaff T, Tekeoglu M, Santra D, Sant VJ, Rajesh PN, Kahl G, Muehlbauer FJ (2000) A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum × C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5. Theor Appl Genet 101:1155–116

    CAS  Google Scholar 

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Acknowledgements

The authors thank to Drs. A. Kleinhofs, S.S. Jones, L.A. Hadwiger, and V.R. Franceschi for critical discussion and manuscript review.

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Author notes

  1. Seungho Cho

    Present address: School of Molecular Biosciences and the Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Washington State University, Pullman, WA 99164-6434, USA

Authors and Affiliations

  1. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA

    Seungho Cho

  2. The Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Washington State University, Pullman, WA 99164-6434, USA

    Weidong Chen

  3. School of Molecular Biosciences and the Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Washington State University, Pullman, WA 99164-6434, USA

    Fred J. Muehlbauer

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  1. Seungho Cho
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  2. Weidong Chen
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  3. Fred J. Muehlbauer
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Correspondence to Fred J. Muehlbauer.

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Communicated by C. Möllers

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Cho, S., Chen, W. & Muehlbauer, F.J. Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight. Theor Appl Genet 109, 733–739 (2004). https://doi.org/10.1007/s00122-004-1693-x

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  • DOI: https://doi.org/10.1007/s00122-004-1693-x

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