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Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa

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Abstract

Although strong intersubgenomic heterosis for seed production has been observed between “natural” domesticated Brassica napus (rapeseed, AACC) and a new type of rapeseed into which subgenomic components of Brassica rapa (AA) have been introgressed, the molecular genetic mechanism of this intersubgenomic heterosis is not understood. In this study, a recombinant inbred line population of new type rapeseed derived from a cross between B. napus and B. rapa, together with a population from a backcross with the parental line of B. napus, was used to identify single-locus quantitative trait locus (QTL) and interacting QTL pairs for yield and nine yield-related traits. More than half of single-locus QTLs and interacting QTL pairs detected were involved with the novel alleles induced by the introgression of B. rapa. The alleles directly from B. rapa A genome played a secondary role in contributing to intersubgenomic heterosis. Allelic and nonallelic interactions of both novel alleles generated by B. rapa introgression and the alleles directly from B. rapa A genome contributed to the intersubgenomic heterosis between “natural” domesticated rapeseed and new type rapeseed into which B. rapa had been introgressed. Six loci for fixed heterosis were identified and their possible applications are also discussed.

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Acknowledgments

This work was supported financially by the National Natural Science Foundation of China (project code: 30830073).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Donghui Fu, Wei Qian, Jun Zou & Jinling Meng

Authors
  1. Donghui Fu
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  2. Wei Qian
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  3. Jun Zou
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  4. Jinling Meng
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Corresponding author

Correspondence to Jinling Meng.

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Supplementary Fig. 1

The detected results of three-locus interacting QTL chain in the TH-BC population and TH-MPH data set Supplementary Fig. 1(a) represents the three-locus interacting QTL chain detected in the TH-BC population and Supplementary Fig. 1(b) denotes the three-locus interacting QTL chain detected in the TH-MPH data set. In each three-locus interacting QTL chain, the characters in the cycles represent the QTL loci and the connected line means the present interacting relationship between the QTLs of two ends of the connected line. Each QTL is denoted by year, followed by trait, linkage group and the index of linked marker, respectively. For example, 05-FT-IP-5-2 means one QTL which was detected in the environment of 2005 year, controlled the flowering time and was closely linked with the No.2 marker in No 5 linkage group. The detected traits included flowering time (FT, days), plant height (PH, cm), branch number per plant (BN), seed number per pod (SN), pod number per plant (PN), 1000-seed weight (SW, g), seed yield (SY, g/plant), economic coefficient (EC), oil yield (OY, g/plant), and dry weight (DW, g/plant). (DOC 58 kb)

Supplementary Fig. 2

Interacting QTL pairs between the A1 and A4 linkage groups detected in the TH-RIL population The QTLs flanked by N-CRB03 in linkage group A4 interacted with the QTLs flanked by N-CRB01 in linkage group A1 to control the pod number per plant, and also interacted with the QTLs flanked by N-CRB02 in linkage group A4 to control dry weight. (DOC 73 kb)

Supplementary Table 1

List of QTLs detected in the TH-BC population and TH-MPH data set (DOC 291 kb)

Supplementary Table 2

List of interacting QTL pairs in the TH-BC population and TH-MPH data set (DOC 1376 kb)

Supplementary Table 3

List of interacting QTL pairs in the TH-RIL population (DOC 752 kb)

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Fu, D., Qian, W., Zou, J. et al. Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa . Euphytica 184, 151–164 (2012). https://doi.org/10.1007/s10681-011-0533-8

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  • DOI: https://doi.org/10.1007/s10681-011-0533-8

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