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A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers

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Abstract

Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria × ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal–Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.

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References

  • Aharoni A, Keizer LCP, Van Den Broeck HC, Blanco-Portales R, Munoz-Blanco J, Bois G, Smit P, De Vos RCH, O’Connell A (2002) Novel insight into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non-climacteric fruit. Plant Physiol 129:1019–1031

    Article  PubMed  CAS  Google Scholar 

  • Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107:1049–1054

    PubMed  CAS  Google Scholar 

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Andersen JR, Lubberstedt T (2003) Functional markers in plants. Trends Plant Sci 8:554–560

    Article  PubMed  CAS  Google Scholar 

  • Arnau G, Lallemand J, Bourgoin M (2003) Fast and reliable strawberry cultivar identification using inter simple sequence repeat (ISSR) amplification. Euphytica 129:69–79

    Article  CAS  Google Scholar 

  • Ashley MV, Wilk JA, Styan SMN, Craft KJ, Jones KL, Feldheim KA, Lewers KS, Ashman TL (2003) High variability and disomic segregation of microsatellites in octoploid Fragaria virginiana Mill (Rosaceae). Theor Appl Genet 107:1201–1207

    Article  PubMed  CAS  Google Scholar 

  • Badawi GH, Yamauchi Y, Shimada E, Sasaki R, Kawano N, Tanaka K, Tanaka K (2004) Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Sci 166:919–928

    Article  CAS  Google Scholar 

  • Bagnoli F, Giannino D, Caparrini S, Camussi A, Mariotti D, Racchi ML (2002) Molecular cloning, characterisation and expression of a manganese superoxide dismutase gene from peach (Prunus persica [L.] Batsch). Mol Genet Genomics 267:321–328

    Article  PubMed  CAS  Google Scholar 

  • Barr HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413–428

    Google Scholar 

  • Bartels D (2001) Targeting detoxification pathways: an efficient approach to obtain plants with multiple stress tolerance. Trends Plant Sci 6:284–286

    Article  PubMed  CAS  Google Scholar 

  • Bartels D, Souer E (2003) Molecular responses of higher plants to dehydration. In: Hirt H, Shinozaki K (eds) Plant responses to abiotic stress. Springer-Verlag, Berlin, pp 9–38

    Google Scholar 

  • Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58

    Article  CAS  Google Scholar 

  • Bassil NV, Gunn M, Folta K, Lewers K (2006) Microsatellite markers for Fragaria from ‘Strawberry Festival’ expressed sequence tags. Mol Ecol Notes 6:473–476

    Article  CAS  Google Scholar 

  • Belaj A, Satovic Z, Cipariani G, Baldoni L, Testolin R, Rallo L, Trujillo I (2003) Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theor Appl Genet 107:736–744

    Article  PubMed  CAS  Google Scholar 

  • Belo A, Zheng P, Luck S, Shen B, Meyer DJ, Li B, Tingey S, Rafalski A (2008) Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize. Mol Genet Genomics 279:1–10

    Article  PubMed  CAS  Google Scholar 

  • Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111

    Article  PubMed  CAS  Google Scholar 

  • Borsani O, Diaz P, Agius MF, Valpuesta C, Monza J (2001) Water stress generates an oxidative stress through the induction of a specific Cu/Zn superoxide dismutase in Lotus corniculatus leaves. Plant Sci 161:757–763

    Article  CAS  Google Scholar 

  • Bray EA (2002) Classification of genes differentially expressed during water-deficit stress in Arabidopsis thaliana: an analysis using microarray and differential expression data. Ann Bot 89:803–811

    Article  PubMed  CAS  Google Scholar 

  • Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. J Exp Bot 55:2331–2341

    Article  PubMed  CAS  Google Scholar 

  • Breseghello F, Sorrells MS (2006) Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172:1165–1177

    Article  PubMed  Google Scholar 

  • Buhariwalla HK, Jayashree B, Eshwar K, Crouch JH (2005) Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus. BMC Plant Biol 5:1–14

    Article  CAS  Google Scholar 

  • Caruso A, Morabito D, Delmotte F, Kahlem G, Carpin S (2002) Dehydrin induction during drought and osmotic stress in Populus. Plant Physiol Biochem 40:1033–1042

    Article  CAS  Google Scholar 

  • Cattivelli L, Rizza F, Badeck FW, Mazzucotelli E, Mastrangelo AM, Francia E, Mare C, Tondelli A, Stanca AM (2008) Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crop Res 105:1–14

    Article  Google Scholar 

  • Causse M, Duffe P, Gomez MC, Buret M, Damidaux R, Zamir D, Gur A, Chevalier C, Lemaire-Chamley M, Rothan C (2004) A genetic map of candidate genes and QTLs involved in tomato fruit size and composition. J Exp Bot 55:1671–1685

    Article  PubMed  CAS  Google Scholar 

  • Chandler CK, Ferree DC (1990) Response of ‘Raritan’ and ‘Surecrop’ strawberry plants to drought stress. Fruit Varieties J 44:183–185

    Google Scholar 

  • Chavez MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought from gene to whole plant. Funct Plant Boil 30:239–264

    Article  Google Scholar 

  • Chen TH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5:250–257

    Article  PubMed  CAS  Google Scholar 

  • Chen X, Salamini F, Gebhardt C (2001) A potato molecular-function map for carbohydrate metabolism and transport. Theor Appl Genet 102:284–295

    Article  CAS  Google Scholar 

  • Clarke JM, McCaig TN (1982) Evaluation of techniques for drought resistance in wheat. Crop Sci 22:503–506

    Article  Google Scholar 

  • Comadran J, Russell JR, van Eeuwijk FA, Ceccarelli S, Grando S, Baum M, Stanca AM, Pecchioni N, Mastrangelo AM, Akar T et al (2008) Mapping adaptation of barley to droughted environments. Euphytica 161:35–45

    Article  Google Scholar 

  • Davik J, Honne BI (2005) Genetic variance and breeding values for resistance to a wind-born disease [Sphaerotheca macularis (Wallr. ex Fr.)] in strawberry (Fragaria × ananassa Duch.) estimated by exploring mixed and spatial models and pedigree information. Theor Appl Genet 111:256–264

    Article  PubMed  CAS  Google Scholar 

  • De Keyser E, Scariot V, Hand T, De Riek J (2006) EST markers in evergreen azalea: development and use for classifications compared to AFLP, microsatellite and morphological data. Acta Hort (ISHS) 763:107–116

    Google Scholar 

  • De Keyser E, De Riek J, Van Bockstaele E (2009) Discovery of species-wide EST-derived markers in Rhododendron by intron-flanking primer design. Mol Breed 23:171–178

    Article  CAS  Google Scholar 

  • De Riek J, Dendauw J, Mertens M, De Loose M, Heursel J, Van Bockstaele E (1999) Validation of criteria for the selection of AFLP markers to assess the genetic variation of a breeders’ collection of evergreen azaleas. Theor Appl Genet 99:1155–1165

    Article  Google Scholar 

  • Degani C, Rowland LJ, Levi A, Hortynski JA, Galletta GJ (1998) DNA fingerprinting of strawberry (Fragaria × ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers. Euphytica 102:247–253

    Article  CAS  Google Scholar 

  • Degani C, Rowland LJ, Saunders JA, Hokanson SC, Ogden EL, Golan-Goldhirsh A, Galleta GJ (2001) A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data. Euphytica 117:1–12

    Article  CAS  Google Scholar 

  • Desikan R, Mackerness SAH, Hancock JT, Neill SJ (2001) Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol 127:159–172

    Article  PubMed  CAS  Google Scholar 

  • Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L, Cosson P, Pronier V, Monet R, Dirlewanger E (2002) Candidate genes and QTLs for sugar and organic acid content in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 105:145–159

    Article  PubMed  CAS  Google Scholar 

  • Flower DJ, Ludlow MM (1986) Contribution of osmotic adjustment to the dehydration tolerance of water-stressed pigeonpox [Cajanus cajan (L.) Millsp.] leaves. Plant Cell Environ 9:33–40

    Google Scholar 

  • Folta KM, Davis TM (2006) Strawberry genes and genomics. Crit Rev Plant Sci 25:399–415

    Article  CAS  Google Scholar 

  • Forster BP, Ellis RP, Moir J, Talame V, Sanguineti MC, Tuberosa R, This D, TeulatMerah B, Ahmed I, Mariy SAEE, Bahri H, El-Ouahabi M, Zoumarou-Wallis N, El-Fellah M, Ben Salem M (2004) Genotype and phenotype associations with drought tolerance in barley tested in North Africa. Ann Appl Biol 144:157–168

    Article  Google Scholar 

  • Fresneau C, Ghashghaie J, Cornic G (2007) Drought effect on nitrate reductase and sucrose-phosphate synthase activities in wheat (Triticum durum L.): role of leaf internal CO2. J Exp Bot 58:2983–2992

    Article  PubMed  CAS  Google Scholar 

  • Garris AJ, McCouch SR, Kresovich S (2003) Population structure and its effects on haplotype diversity and linkage disequilibrium surrounding the xa5 locus of rice Oryza sativa L. Genetics 165:759–769

    PubMed  Google Scholar 

  • Gehrman H, Lenz FR (1991) Wassersbedrijf und einfluss von wassermangel bei erdbeere. I. Blattfl achenentwicklung und trockensubstanzverteilung. Erwerbsobstbau 33:14–17

    Google Scholar 

  • Gil-Ariza DJ, Amaya I, Botella MA, Munoz Blanco J, Caballero JL, Lopez-Aranda JM, Valpuesta V, Sanchez-Sevilla JF (2006) EST-derived polymorphic microsatellites from cultivated strawberry (Fragaria × ananassa) are useful for diversity studies and varietal identification among Fragaria species. Mol Ecol Notes 6:1195–1197

    Article  CAS  Google Scholar 

  • González-Martínez SC, Ersoz E, Brown GR, Wheeler NC, Neale DB (2006) DNA sequence variation and selection of tag single-nucleotide polymorphisms at candidate genes for drought-stress response in Pinus taeda L. Genetics 172:1915–1926

    Article  PubMed  CAS  Google Scholar 

  • Govan CL, Simpson DW, Johnson AW, Tobutt KR, Sargent DJ (2008) A reliable multiplexed microsatellite set for genotyping Fragaria and its use in a survey of 60 F. × ananassa cultivars. Mol Breed 22:649–661

    Article  CAS  Google Scholar 

  • Graham J, McNichol RJ, McNichol JW (1996) A comparison of methods for the estimation of genetic diversity in strawberry cultivars. Theor Appl Genet 93:402–406

    Article  CAS  Google Scholar 

  • Gupta PK, Rustgi S (2004) Molecular markers from the transcribed/expressed region of the genome in higher plants. Funct Integr Genomics 4:137–162

    Article  CAS  Google Scholar 

  • Gupta PK, Rustgi S, Sharma S, Singh R, Kumar N, Balyam HS (2003) Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol Genet Genomics 270:315–323

    Article  PubMed  CAS  Google Scholar 

  • Gupta PK, Rustgi S, Kulwal PL (2005) Linkage disequilibrium and association studies in higher plants: present status and future prospects. Plant Mol Biol 57:461–485

    Article  PubMed  CAS  Google Scholar 

  • Haake V, Cook D, Riechmann JL, Pineda O, Thomashow MF, Zhang JZ (2002) Transcription factor CBF4 is a regular of drought adaptation in Arabidopsis. Plant Physiol 130:639–648

    Article  PubMed  CAS  Google Scholar 

  • Hall D, Tegstrom C, Ingvarsson PK (2010) Using association mapping to dissect the genetic basis of complex traits in plants. Brief Funct Genomics 9:157–165

    Article  PubMed  CAS  Google Scholar 

  • Hamblin MT, Mitchell SE, White GM, Gallego J, Kukatla R, Wing RA, Paterson AH, Kresovich S (2004) Comparative population genetics of the panicoid grasses: sequence polymorphism, linkage disequilibrium and selection in a diverse sample of Sorghum bicolor. Genetics 167:471–483

    Article  PubMed  CAS  Google Scholar 

  • Hare PD, Cress WA, Van Staden J (1999) Proline synthesis and degradation: a model system for stress-related signal transduction. J Exp Bot 50:413–434

    Article  CAS  Google Scholar 

  • Hegde VS, Mishra SK (2009) Landraces of cowpea Vigna unguiculata (L.) Walp., as potential sources of genes for unique characters in breeding. Genet Resour Crop Evol 56:615–627

    Article  CAS  Google Scholar 

  • Hietaranta T, Linna MM (1997) Selecting cultivars for strawberry production in Finland—clone and cultivar trials. Acta Hort (ISHS) 439:145–148

    Google Scholar 

  • Horvath GV, Turoczy Z, Cserhati M, Kis P, Dudits D, Torok K, Sass L, Dudits D (2006) Engineering of reactive species detoxification pathways for increasing stress tolerance in plants. In: Xu Z et al (eds) Biotechnology and sustainable agriculture 2006 and beyond. Springer, Dordrecht, pp 163–166

    Google Scholar 

  • Hubbard NL, Pharr DM, Huber SC (2006) Sucrose phosphate synthase and other sucrose metabolizing enzymes in fruits of various species. Physiol Plant 82:191–196

    Article  Google Scholar 

  • Hyten DL, Choi IY, Song Q, Shoemaker RC, Nelson RL, Costa JM, Specht JE, Cregan PB (2007) Highly variable patterns of linkage disequilibrium in multiple soybean populations. Genetics 175:1937–1944

    Article  PubMed  CAS  Google Scholar 

  • Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Plant Physiol Plant Mol Biol 47:377–403

    Article  CAS  Google Scholar 

  • Ingram J, Chandler JW, Gallagher L, Salamini F, Bartels D (1997) Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craferosfigma planfagineum Hochst. Plant Physiol 115:113–121

    Article  PubMed  CAS  Google Scholar 

  • Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K (2001) Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis. Plant J 27:325–333

    Article  PubMed  CAS  Google Scholar 

  • Ivandic V, Thomas WTB, Nevo E, Zhang Z, Froster BP (2003) Association of simple sequence repeats with quantitative trait variation including biotic and abiotic stress tolerance in Hordeum spontaneum. Plant Breed 122:300–304

    Article  CAS  Google Scholar 

  • Jung S, Jesudurai C, Staton M, Du Z, Ficklin S, Cho I, Abbott A, Tomkins J, Main D (2004) GDR (Genome Database for Rosaceae): integrated web resources for Rosaceae genomics and genetics research. BMC Bioinform 5:130

    Article  CAS  Google Scholar 

  • Jung S, Staton M, Lee T, Blenda A, Svancara R, Abbott A, Main D (2008) GDR (Genome Database for Rosaceae): integrated web-database for Rosaceae genomics and genetics data. Nucleic Acids Res 36:D1034–D1040

    Article  PubMed  CAS  Google Scholar 

  • Kashyap S, Kaur R, Kumar K (2005) Molecular characterization and genetic diversity in Fragaria genotypes as revealed by randomly amplified DNA polymorphisms. Acta Hort (ISHS) 696:135–142

    Google Scholar 

  • Kepenek K, Koyuncu F (2002) Studies on the salt tolerance of some strawberry cultivars under glasshouse. Acta Hort (ISHS) 573:297–304

    Google Scholar 

  • Keutgen AJ, Pawelzik E (2007) Modifications of strawberry fruit antioxidant pools and fruit quality under NaCl stress. J Agric Food Chem 55:4066–4072

    Article  PubMed  CAS  Google Scholar 

  • Kim IJ, Lee BH, Jo J, Chung WI (2001) Sequence variability of nine cytosolic ascorbate peroxidases in polyploid strawberry. DNA Seq 11:475–484

    PubMed  Google Scholar 

  • Klamkowski K, Treder W (2006) Morphological and physiological responses of strawberry plants to water stress. Agric Conspec Sci 71:159–165

    Google Scholar 

  • Klamkowski K, Treder W (2008) Response to drought stress of three strawberry cultivars grown under greenhouse conditions. J Fruit Ornam Plant Res 16:179–188

    Google Scholar 

  • Korbin M, Kuras A, Zurawicz E (2002) Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR. Cell Mol Biol Lett 7:785–794

    PubMed  CAS  Google Scholar 

  • Kraakman ATW, Niks RE, Van der Berg PMMM, Stam P, Van Eeuwijk FA (2004) Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics 168:435–446

    Article  PubMed  CAS  Google Scholar 

  • Kraakman ATW, Martinez F, Mussiraliev B, Van Eeuwijk FA, Niks RE (2006) Linkage disequilibrium mapping of morphological, resistance, and other agronomically relevant traits in modern spring barley cultivars. Mol Breed 17:41–58

    Article  CAS  Google Scholar 

  • Kuras A, Korbin M, Zurawicz E (2004) Comparison of suitability of RAPD and ISSR techniques for determination of strawberry (Fragaria × ananassa) relationship. Plant Cell Tiss Organ Cult 79:189–193

    Article  CAS  Google Scholar 

  • Lee YP, Kim SH, Bang JW, Lee HS, Kwak SS, Kwon SY (2007) Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Plant Cell Rep 26:591–598

    Article  PubMed  CAS  Google Scholar 

  • Liu X, Baird WV (2003) Differential expression of genes regulated in response to drought or salinity stress in sunflower. Crop Sci 43:678–687

    CAS  Google Scholar 

  • Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406

    Article  PubMed  CAS  Google Scholar 

  • Llop-Tous I, Dominguez-Puigjaner E, Vendrell M (2002) Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature. J Exp Bot 53:2283–2285

    Article  PubMed  CAS  Google Scholar 

  • Luna CM, M Pastori G, Driscoll S, Groten K, Bernard SH, Foyer C (2004) Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. J Exp Bot 56:417–423

    Article  PubMed  CAS  Google Scholar 

  • Malatrasi M, Close TJ, Marmiroli N (2002) Identification and mapping of a putative stress response regulator gene in barley. Plant Mol Biol 50:143–152

    Article  PubMed  CAS  Google Scholar 

  • Malysheva-Otto L, Roder MS (2006) Haplotype diversity in the endosperm specific β-amylase gene βmy 1 of cultivated barley (Hordeum vulgare L.). Mol Breed 18:143–156

    Article  CAS  Google Scholar 

  • Mantel N (1967) The detection of disease clustering and generalized regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • Medina-Escobar N, Gardenas J, Munoz-Blanco J, Caballero JL (1998) Cloning and molecular characterization of a strawberry fruit ripening-related cDNA corresponding a mRNA for a low-molecular-weight heat-shock protein. Plant Mol Biol 36:33–42

    Article  PubMed  CAS  Google Scholar 

  • Milella L, Saluzzi D, Lapelosa M, Bertino G, Spada P, Greco I, Martelli G (2006) Relationships between an Italian strawberry ecotype and its ancestor using RAPD markers. Genet Resour Crop Evol 53:1715–1720

    Article  CAS  Google Scholar 

  • Mishra RK, Sen-Mandi S (2004) Molecular profiling and development of DNA marker associated with drought tolerance in tea clones growing in Darjeeling. Curr Sci 87:60–66

    CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498

    Article  PubMed  CAS  Google Scholar 

  • Muthurajan R, Shobbar ZS, Jagadish SVK, Bruskiewich R, Ismail A, Leung H, Bennett J (2010) Physiological and proteomic responses of rice peduncles to drought stress. Mol Biotechnol. doi:10.1007/s12033-010-9358-2

  • Nakashima K, Yamaguchi-Shinozaki K (2005) Molecular studies on stress-responsive gene expression in Arabidopsis and improvement of stress tolerance in crop plants by regulon biotechnology. JARQ 39:221–225

    CAS  Google Scholar 

  • Nakashima K, Yamaguchi-Shinozaki K (2006) Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants. Physiol Plant 126:62–71

    Article  CAS  Google Scholar 

  • Ndong C, Ouellet F, Houde M, Sarhan F (1997) Gene expression during cold acclimation in strawberry. Plant Cell Physiol 38:863–870

    PubMed  CAS  Google Scholar 

  • Oberschall A, Deak M, Török K, Saa L, Vass I, Kovacs I, Feher A, Dudits D, Horvath GV (2000) A novel aldose/aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses. Plant J 24:437–446

    Article  PubMed  CAS  Google Scholar 

  • Owens CL, Thomashow MF, Hanckok JF, Iezzoni AF (2002) CBF1 orthologs in sour cherry and strawberry and the heterologous expression of CBF1 in strawberry. J Am Soc Hort Sci 127:462–710

    Google Scholar 

  • Park SY, Ryu SH, Jang IC, Kwon SY, Kim JG, Kwak SS (2004) Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweet potato and its expression in response to stress. Mol Gen Genomics 271:339–346

    Article  CAS  Google Scholar 

  • Pearce RG (1999) Molecular analysis of acclimation to cold. Plant Growth Regul 29:47–76

    Article  CAS  Google Scholar 

  • Peleg Z, Fahima T, Abbo S, Krugman T, Nevo E, Yakir D, Saranga Y (2005) Genetic diversity for drought resistance in wild emmer wheat and its ecogeographical associations. Plant, Cell Environ 28:176–191

    Article  Google Scholar 

  • Puhakainen T, Hess MW, Makela P, Svensson J, Heino P, Tapio Palva E (2004) Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis. Plant Mol Biol 54:743–753

    Article  PubMed  CAS  Google Scholar 

  • Qian C, Liu S, Yin K (2005) Effects of water stress on the membrane protection system of strawberry. J Southwest Agric Univ. doi:CNKI:SUN:XNND.0.2005-04-029

  • Quick P, Siegl G, Neuhaus E, Feil R, Stitt M (1989) Short-term water stress leads to a stimulation of sucrose synthesis by activating sucrose-phosphate synthase. Planta 177:535–546

    Article  CAS  Google Scholar 

  • Rampino P, Pataleo S, Gerardi C, Mita G, Perrota C (2006) Drought stress response in wheat: physiological and molecular analysis of tolerant and sensitive genotypes. Plant Cell Environ 29:2143–2152

    Article  PubMed  CAS  Google Scholar 

  • Ravel C, Praud S, Murigneux A, Linossier L, Dardevet M, Balfourier F, Dufour PH, Brunel D, Charmet G (2006) Identification of Glu-B1-1 as a candidate gene for the quantity of high-molecular-weight glutenin in bread wheat (Triticum aestivum L.) by means of an association study. Theor Appl Genet 112:738–743

    Article  PubMed  CAS  Google Scholar 

  • Razavi F, Pollet B, Steppe K, Van Labeke MC (2008) Study of chlorophyll fluorescence as a tool for evaluation of drought stress in strawberry. Photosynthetica 46:631–633

    Article  CAS  Google Scholar 

  • Remington DL, Thornsberry JM, Matsuoka Y, Wilson LM, Whitt SR, Doebley J, Kresovich S, Goodman MM, Buckler ES IV (2001) Structure of linkage disequilibrium and phenotypic association in the maize genome. PNAS 98:11479–11484

    Article  PubMed  CAS  Google Scholar 

  • Rhone B, Raquin AL, Goldringer I (2007) Strong linkage disequilibrium near the selected Yr17 resistance gene in a wheat experimental population. Theor Appl Genet 114:787–802

    Article  PubMed  CAS  Google Scholar 

  • Ristic Z, Jenks MA (2002) Leaf cuticle and water loss in maize lines differing in dehydration avoidance. J Plant Physiol 159:645–651

    Article  CAS  Google Scholar 

  • Rohloff J, Bones AM, Winge P, Kopka J, Erban A, Sparstad T, Wilson RC, Randall SK, Davik J, Alsheikh M (2009) Metabolic, transcriptional and proteomics profiling of cold response in Fragaria vesca. Acta Hort (ISHS) 842:785–788

    Google Scholar 

  • Rostoks N, Ramsay L, MacKenize K, Cardle L, Bhat PR, Roose ML, Svensson JT, Stein N, Varshney RK, Marshall DF et al (2006) Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties. PNAS 103:18656–18661

    Article  PubMed  CAS  Google Scholar 

  • Saiful Islam M, Wang MH (2009) Expression of dehydration responsive element-binding protein-3 (DREB3) under different abiotic stresses in tomato. BMB Rep, 611–616

  • Saleh A, Lumreras V, Pages M (2005) Functional role of DRE-binding transcription factors in abiotic stress. In: Tuberosa R, Phillips RL, Gale M (eds) Proceedings of the international congress “In the wake of the double helix: from the green revolution to the gene revolution”. Avenue Media, Bologna, Italy, pp 193–205

  • Sargent DJ, Hadonou AM, Simpson DW (2003) Development and characterisation of polymorphic microsatellite markers from Fragaria viridis, a wild diploid strawberry. Mol Ecol Notes 3:550–552

    Article  CAS  Google Scholar 

  • Sargent DJ, Davis TM, Tobutt KR, Wilkinson MJ, Battey NH, Simpson DW (2004) A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theor Appl Genet 109:1385–1391

    Article  PubMed  CAS  Google Scholar 

  • Sasaki H, Ichimura K, Imada S, Yamaki S (2001) Sucrose synthase and sucrose phosphate synthase, but not acid invertase, are regulated by cold acclimation and deacclimation in cabbage seedlings. J Plant Physiol 158:847–852

    Article  CAS  Google Scholar 

  • Scariot V, De Keyser E, Handa T, De Riek J (2007) Comparative study of the discriminating capacity and effectiveness of AFLP, STMS and EST markers in assessing genetic relationship among evergreen azaleas. Plant Breed 126:207–212

    Article  CAS  Google Scholar 

  • Schwab W, G Schaart J, Rosati C (2009) Functional molecular biology research in Fragaria. In: Folta KM, Gardiner SE (eds) Genetic and genomics of Rosaceae. Springer, New York, pp 457–486

    Google Scholar 

  • Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high salinity stresses using a full-length cDNA microarray. Plant J 31:279–292

    Article  PubMed  CAS  Google Scholar 

  • Shao HB, Liang ZS, Shao MA, Sun Q (2005) Dynamic changes of anti-oxidative enzymes of 10 wheat genotypes at soil water deficits. Biointerfaces 42:187–195

    Article  PubMed  CAS  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227

    Article  PubMed  CAS  Google Scholar 

  • Shulaev V, Korban SS, Sosinski B, Abbott AG, Aldwinckle HS, Folta KM, Iezzoni A, Main D, Arús P, Dandekar AM, Lewers K, Brown SK, Davis TM, Gardiner SE, Potter D, Veilleux RE (2008) Multiple models for Rosaceae genomics. Plant Physiol 147:985–1003

    Article  PubMed  CAS  Google Scholar 

  • Simko I, Costanzo S, Haynes KG, Chris BJ, Jones RW (2004) Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato Solanum tuberosum through a candidate gene approach. Theor Appl Genet 108:217–224

    Article  PubMed  CAS  Google Scholar 

  • Skot L, Humphreys MO, Armstead I, Heywood S, Skot KP, Sanderson R, Thomas ID, Sanderson R, Chorlton KH, Hamilton NRS (2005) An association mapping approach to identify flowering time genes in natural populations of Lotium perenne (L.). Mol Breed 15:233–245

    Article  CAS  Google Scholar 

  • Smouse PE, Long JC, Sokal RR (1986) Multiple regression and correlation extension of the Mantel test of Matrix correspondence. Syst Zool 35:627–632

    Article  Google Scholar 

  • Sorkheh K, Malysheva-Otto LV, Wirthensohn MG, Tarkesh-Esfahani S, Martinez-Gomez P (2008) Linkage disequilibrium, genetic association mapping and gene localization in crop plants. Genet Mol Biol 31:805–814

    Article  Google Scholar 

  • Sturm A (1999) Invertases, primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol 121:1–7

    Article  PubMed  CAS  Google Scholar 

  • Sunkar R, Bartels (2002) Drought- and desiccation-induced modulation of gene expression in plants. Plant Cell Environ 25:141–151

    Article  Google Scholar 

  • Suprunova T, Krugman T, Fahima T, Chen G, Shams I, Korol A, Nevo E (2004) Differential expression of dehydrin genes in wild barley, Hordeum spontaneum, associated with resistance to water deficit. Plant Cell Environ 27:1297–1308

    Article  CAS  Google Scholar 

  • Teulat B, Zoumarou-Wallis N, Rotter B, Ben Salem M, Bahri H, This D (2003) QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theor Appl Genet 108:181–188

    Article  PubMed  CAS  Google Scholar 

  • Thornsberry JM, Goodman MM, Doebley J, Kresovich S, Nielsen D, Buckler ES IV (2001) Dwarf8 polymorphism associated with variation in flowering time. Nat Genet 28:286–289

    Article  PubMed  CAS  Google Scholar 

  • Tommasini L, Schnurbusch T, Fossati D, Mascher F, Keller B (2007) Association mapping of Stagonospora nodorum blotch resistance in modern European winter wheat varieties. Theor Appl Genet 115:697–708

    Article  PubMed  CAS  Google Scholar 

  • Tuberosa R, Salvi S (2006) Genomics-based approaches to improve drought tolerance of crops. Trends Plant Sci 11:405–412

    Article  PubMed  CAS  Google Scholar 

  • Turhan E, Gulen H, Eris A (2008) The activity of antioxidative enzymes in three strawberry cultivars related to salt-stress tolerance. Acta Physiol Plant 30:201–208

    Article  CAS  Google Scholar 

  • Tyrka M, Dziadczyk P, Hortynski JA (2002) Simplified AFLP procedure as a tool for identification of strawberry cultivars and advanced breeding lines. Euphytica 125:273–280

    Article  CAS  Google Scholar 

  • VanDerZanden AM, Cameron SJ (1996) Effect of water deficit stress on 11 native Fragaria chiloensis clones selected as ornamental groundcovers. Sci Hort 66:241–253

    Article  Google Scholar 

  • Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu JK (2006) Methods and concept in quantifying resistance to drought, salt and freezing, abiotic stressed that affect plant water status. Plant J 45:523–539

    Article  PubMed  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Fijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 21:4407–4414

    Article  Google Scholar 

  • Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14

    Article  PubMed  CAS  Google Scholar 

  • Wei W, Dai X, Wang Y, Chuan Y, Gou CB, Chen F (2010) Cloning and expression analysis of 1 L-myo-Inositol-1-phosphate synthase gene from Ricinus communis L. Z Naturforsch 65c:501–507

    Google Scholar 

  • Winter H, Huber SC (2000) Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Crit Rev Plant Sci 19:31–67

    Article  CAS  Google Scholar 

  • Wisniewski ME, Basset CL, Renaut J, Farrell R, Tworkoski T, S.Artlip T (2006) Differential regulation of two dehydrin genes from peach (Prunus persica) by photoperiod, low temperature and water deficit. Tree Physiol 26:575–584

    PubMed  CAS  Google Scholar 

  • Xiao H, Siddiqua M, Braybrook S, Nassuth A (2006) Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid. Plant Cell Environ 29:1410–1421

    Article  PubMed  CAS  Google Scholar 

  • Xing Y, Frei U, Schejbel B, Asp T, Lubberstedt T (2007) Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in Lolium perenne. BMC Plant Biol 7:43

    Article  PubMed  CAS  Google Scholar 

  • Xiong LM, Lee HJ, Ishitani M, Zhu JK (2002) Regulation of osmotic stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis. J Biol Chem 277:8588–8596

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57:781–803

    Article  PubMed  CAS  Google Scholar 

  • Yamaki S (1995) Physiology and metabolism of fruit development—biochemistry of sugar metabolism and compartmentation in fruits. Acta Hort (ISHS) 398:109–120

    CAS  Google Scholar 

  • Yu J, Buckler ES (2006) Genetic association mapping and genome organization of maize. Curr Opin Biotechnol 17:155–160

    Article  PubMed  CAS  Google Scholar 

  • Yushiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi-Shinozaki K, Wada K, Harada Y, Shinozaki K (1995) Correlation between the induction of a gene for Δ1-pyrroline-5-carboxylate synthetase and accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant J 7:751–760

    Article  Google Scholar 

  • Zhang B, Archbold DD (1993) Solute accumulation in leaves of a Fragaria chiloensis and a F. virginiana selection responds to water deficit stress. J Am Soc Hort Sci 118:280–285

    CAS  Google Scholar 

  • Zhang Y, Shih DS (2007) Isolation of an osmotin-like protein gene from strawberry and analysis of the response of this gene to abiotic stresses. J Plant Physiol 164:68–77

    Article  PubMed  CAS  Google Scholar 

  • Zhang JZ, Creelman RA, Zhu JK (2004) From laboratory to field using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol 135:615–621

    Article  PubMed  CAS  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  PubMed  CAS  Google Scholar 

  • Zhu C, Gore M, S.Buckler E, Yu J (2008) Status and prospects of association mapping in plants. Plant Genome 1:5–20

    Article  CAS  Google Scholar 

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Acknowledgments

We gratefully acknowledge the financial support of the Iranian Ministry of Science, Research and Technology in this research by means of the scholarship granted to the first author and also the financial support of Ghent University by means of the BOF (Bijzonder Onderzoeksfonds) granted to the first author. The authors thank Veerle Buysens for her assistance in DNA preparations and AFLP and EST reactions. The whole staff of the biotech lab of ILVO (Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding) was very much appreciated for their support and skilful assistance. Special thanks to ir. Sarsaifee M., Agricultural Research Center, Kurdistan, Iran.

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Authors and Affiliations

  1. Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Farzaneh Razavi & Marie Christine Van Labeke

  2. ILVO, Institute for Agricultural & Fisheries Research, Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium

    Ellen De Keyser & Jan De Riek

  3. Iranian Ministry of Science, Research & Technology and Ministry of Agriculture, Tehran, Iran

    Farzaneh Razavi

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  1. Farzaneh Razavi
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  2. Ellen De Keyser
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  3. Jan De Riek
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  4. Marie Christine Van Labeke
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Correspondence to Farzaneh Razavi.

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Razavi, F., De Keyser, E., De Riek, J. et al. A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. Euphytica 180, 385–409 (2011). https://doi.org/10.1007/s10681-011-0398-x

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