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A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria

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Abstract

Diploid Fragaria provide a potential model for genomic studies in the Rosaceae. To develop a genetic linkage map of diploid Fragaria, we scored 78 markers (68 microsatellites, one sequence-characterised amplified region, six gene-specific markers and three morphological traits) in an interspecific F2 population of 94 plants generated from a cross of F.vesca f. semperflorens × F. nubicola. Co-segregation analysis arranged 76 markers into seven discrete linkage groups covering 448 cM, with linkage group sizes ranging from 100.3 cM to 22.9 cM. Marker coverage was generally good; however some clustering of markers was observed on six of the seven linkage groups. Segregation distortion was observed at a high proportion of loci (54%), which could reflect the interspecific nature of the progeny and, in some cases, the self-incompatibility of F. nubicola. Such distortion may also account for some of the marker clustering observed in the map. One of the morphological markers, pale-green leaf (pg) has not previously been mapped in Fragaria and was located to the mid-point of linkage group VI. The transferable nature of the markers used in this study means that the map will be ideal for use as a framework for additional marker incorporation aimed at enhancing and resolving map coverage of the diploid Fragaria genome. The map also provides a sound basis for linkage map transfer to the cultivated octoploid strawberry.

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Acknowledgements

This research was supported by funds from the University of Reading Research Endowment Trust Fund, the East Malling Trust for Horticultural Research the Worshipful Company of Fruiterers and Defra. We are grateful to Radovan Bošković and Mike Solomon for critically reviewing the manuscript and to Asaph Aharoni for the provision of PCR primers for FvNES1.

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Authors and Affiliations

  1. East Malling Research, New Road, East Malling, West Malling, Kent ME19 6BJ, UK

    D. J. Sargent, K. R. Tobutt & D. W. Simpson

  2. School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, Reading RG6 6AS, UK

    D. J. Sargent, M. J. Wilkinson & N. H. Battey

  3. Plant Biology Department, University of New Hampshire, Durham, NH 03824, USA

    T. M. Davis

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  1. D. J. Sargent
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  2. T. M. Davis
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  3. K. R. Tobutt
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  4. M. J. Wilkinson
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  5. N. H. Battey
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  6. D. W. Simpson
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Correspondence to D. J. Sargent.

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Communicated by I. Paran

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Sargent, D.J., Davis, T.M., Tobutt, K.R. et al. A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theor Appl Genet 109, 1385–1391 (2004). https://doi.org/10.1007/s00122-004-1767-9

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  • DOI: https://doi.org/10.1007/s00122-004-1767-9

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