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Genetic dissection of a chromosomal region conferring hybrid sterility using multi-donors from Oryza glaberrima

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Abstract

A chromosomal region containing a hybrid sterility locus, S 1, from Oryza glaberrima, located on the short arm of chromosome 6, was investigated in 15 BC7F1 and one BC3F2 populations. As a gamete eliminator, S 1 induced both male and female gamete abortion and responded to typical, spherical, or empty pollen abortion when it was heterozygous. The gametes carrying S a1 (Oryza sativa allele in S 1) in the heterozygous S 1 locus had been eliminated in previous studies. We found here that both male and female gametes carrying the S a1 allele were killed incompletely in heterozygous (S g1 S a1 ) plants, with 26.22% female and 9.06% male gametes with the S a1 genotype remaining when the BC3F1 sterile plant produced male and female gametes, which fits with the hypothesis that the ratio of S a1 gametes of the female that were killed is higher than that of the male gametes. In addition, in sterile BC6F1 plants with the heterozygous S 1 locus, not only a majority of the female gametes carrying the S a1 allele, but also a quite number of those carrying the S g1 allele (O. glaberrima allele in S 1) could not be transmitted to their offspring. This abnormal transmission of female gametes illustrates the complexity of the reproductive isolation mechanism caused by S 1 and can not be explained by the ‘one locus, sporo-gametal interaction’ model alone. Interaction for female sterility between S 1 and two other loci on chromosomes 1 and 4 that suppress the expression of S 1 was identified. S 1 is the main reproductive isolation locus when mining favorable traits/genes from O. glaberrima due to its ubiquity between these two species, and the development of bridge parents carrying S 1 should be a useful method for overcoming or abating this obstacle between these two species. The chromosomal region of S 1 may be the hot region of genetic diversity for speciation because several hybrid sterility loci between species, subspecies, and even intra-subspecies have been identified in the same region.

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Acknowledgments

This research was funded partially by grants from Ministry of Science and Technology (2006CB708207), and Yunnan Department of Science and Technology (2002C0009Z, 2003RC02, 2004PY01-21, 2006GP09, and 2006C0063M), People’s Republic of China.

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Authors and Affiliations

  1. Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Jiawu Zhou, Zhigang Zhao, Ling Jiang & Jianmin Wan

  2. Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, People’s Republic of China

    Jiawu Zhou, Peng Xu, Xianneng Deng, Jing Li, Fengyi Hu, Wei Deng & Dayun Tao

  3. Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Guangyun Ren, Zhi Zhang & Yahong Luan

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  1. Jiawu Zhou
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  2. Peng Xu
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  3. Xianneng Deng
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  4. Jing Li
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  5. Fengyi Hu
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  10. Zhigang Zhao
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  12. Jianmin Wan
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  13. Dayun Tao
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Correspondence to Jianmin Wan or Dayun Tao.

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Zhou, J., Xu, P., Deng, X. et al. Genetic dissection of a chromosomal region conferring hybrid sterility using multi-donors from Oryza glaberrima . Euphytica 175, 395–407 (2010). https://doi.org/10.1007/s10681-010-0188-x

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  • DOI: https://doi.org/10.1007/s10681-010-0188-x

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