Skip to main content
SpringerLink
Log in

Fine mapping of S31, a gene responsible for hybrid embryo-sac abortion in rice (Oryza sativa L.)

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

Partial abortion of female gametes and the resulting semi-sterility of indica × japonica inter-subspecific rice hybrids have been ascribed to an allelic interaction, which can be avoided by the use of wide compatibility varieties. To further understand the genetic mechanism of hybrid sterility, we have constructed two sets of hybrids, using as male parent either the typical japonica variety Asominori, or the wide compatibility variety 02428; and as female, a set of 66 chromosome segment substitution lines in which various chromosomal segments from the indica variety IR24 have been introduced into a common genetic background of Asominori. Spikelet semi-sterility was observed in hybrid between CSSL34 and Asominori, which is known to carry the sterility gene S31 (Zhao et al. in Euphytica 151:331–337, 2006). Cytological analysis revealed that the semi-sterility of the CSSL34 × Asominori hybrid was caused primarily by partial abortion of the embryo sac at the stage of the mitosis of the functional megaspore. A population of 1,630 progeny of the three-way cross (CSSL34 × 02428) × Asominori was developed to map S31. Based on the physical location of linked molecular markers, S31 was thereby delimited to a 54-kb region on rice chromsome 5. This fragment contains eight predicted open reading frames, four of which encode known proteins and four putative proteins. These results are relevant to the map-based cloning of S31, and the development of marker-assisted transfer of non-sterility allele inducing alleles to breeding germplasm, to allow for a more efficient exploitation of heterosis in hybrid rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

CSSLs:

Chromosome segment substitution lines

ORF:

Open reading frame

SSR:

Simple sequence repeat

WCG:

Wide compatibility gene

WCLSM:

Whole-mount stain-clearing laser scanning confocal microscopy

WCV:

Wide compatibility variety

References

  • Bouharmont J, Oliver M, Dumont D, Chassart M (1985) Cytological observations in some hybrids between the rice species Oryza sativa L. and O. glaberrima Steud. Euphytica 34:75–81

    Article  Google Scholar 

  • Chen X, Temnykh S, Xu Y, Cho YG, McCouch SR (1997) Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor Appl Genet 95:553–567

    Article  CAS  Google Scholar 

  • Daniela N, Schreiber, Thomas D (2003) In vitro pollen germination and transient transformation of zea mays and other plant species. Plant Mol Biol Report 21:31–41

    Google Scholar 

  • Dellapporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: version II. Plant Mol Biol Rep 1:19–21

    Google Scholar 

  • Eshed Y, Zamir D (1995) An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141:1147–1162

    PubMed  CAS  Google Scholar 

  • Frary An, Nesbitt TC, Frary Am, Grandillo S, Knaap EVD, Cong B, Liu JP, Meller R, Alpert KB, Tanksley SD (2000) fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289:85–88

    Article  Google Scholar 

  • Freshour G, Clay RP, Fuller MS, Albersheim P, Darvill AG, Hahn MG (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol 110:1413–1429

    PubMed  CAS  Google Scholar 

  • Ikehashi H (1991) Genetics of hybrid sterility in wide hybridization in rice. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 14, pp 113–127

  • Ikehashi H, Araki H (1986) Genetics of F1 sterility in remote crosses of rice. In: IRRI (ed) Rice genetics. IRRI, Manila, pp 119–130

  • Kazimierska EM, Kazimierska T (1995) Cytoembryology of infertile segregants of the hybrid Lupinus varius. × L. digitatus Forsk. J Appl Genet 36:215–227

    Google Scholar 

  • Kubo T, Nakamura K, Yoshimura A (1999) Development of a series of indica chromosome segment substitution lines in japonica background of rice. Rice Genet Newsl 16:104–106

    Google Scholar 

  • Kubo T, Aida Y, Nakamura K, Tsunematsu H, Doi K, Yoshimura A (2002) Reciprocal chromosome segment substitution series derived from japonica and indica cross of rice (Oryza sativa L.). Breed Sci 52:319–325

    Article  CAS  Google Scholar 

  • Lao N, Long D, Kiang S, Coupland G, Shoue D, Carpita N, Kavanagh T (2003) Mutation of a family 8 glycosyltransferase gene alters cell wall carbohydrate composition and causes a humidity-sensitive semi-sterile dwarf phenotype in Arabidopsis. Plant Mol Biol 53:687–701

    Article  PubMed  Google Scholar 

  • Law CN (1966) The location of genetic factors affecting a quantitative character in wheat. Genetics 53:487–498

    PubMed  Google Scholar 

  • Li HB, Wang J, Liu AM, Liu KD, Zhang Q, Zou JS (1997) Genetic basis of low-temperature-sensitive sterility in indica-japonica hybrid of rice as determined by RFLP analysis. Theor Appl Genet 95:1092–1097

    Article  CAS  Google Scholar 

  • Li WT, Zeng RZ, Zhang ZM, Ding XH, Zhang GQ (2006) Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.). Chin Sci Bull 51:675–680

    Article  CAS  Google Scholar 

  • Liu YS, Zhu LH, Sun JS, Chen Y (2001) Mapping QTLs for defective female gametophyte development in an inter-subspecific cross in Oryza sativa L. Theor Appl Genet 102:1243–1251

    Article  CAS  Google Scholar 

  • Liu HY, Xu CG, Zhang QF (2004a) Male and female gamete abortions, and reduced affinity between the uniting gametes as the causes for sterility in an indica/japonica hybrid in rice. Sex Plant Rep 17:55–62

    Google Scholar 

  • Liu XD, Lu YG, Zhu HL, Xu XB, Feng JH, Xu SX (2004b) Abnormal behavior of nuclei and microrubule (MT) organizational changes during embryo sac development in the poly-egg mutant, AP IV of rice. Acta Bot Sin 46:829–838

    CAS  Google Scholar 

  • McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing YZ, Zhang Q, Kono I, Yano M, Fjellstrom R, Declerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  PubMed  CAS  Google Scholar 

  • Ouyang XZ (1996) Ultrastructure and ACPase ultracytochemical localization of abortive functional megaspores in intersubspecific F1 hybrids rice (Oryza sativa L.). Chin Rice Res Newsl 4:2–3

    Google Scholar 

  • Qiu SQ, Liu KD, Jiang JX, Song X, Xu CG, Li XH, Zhang QF (2005) Delimitation of the rice wide compatibiity gene S5n to a 40-kb DNA fragment. Theor Appl Genet 111:1080–1086

    Article  PubMed  CAS  Google Scholar 

  • Reiter WD, Chaple C, Somerville CR (1997) Mutants of Arabidopsis thaliana with altered cell wall polysaccharide composition. Plant J 12:335–345

    Article  PubMed  CAS  Google Scholar 

  • Rim YW, Beuselinck PR, Mcgraw RL (1998) Megagametophyte development in Lotus corniculatus × L. conimbricensis and their protoplast fusion hybrid. Lotus Newsl 19:45–47

    Google Scholar 

  • Sanguinetti CJ, Dias NE, Simpson AJG (1994) Rapid silver staining and recover of PCR products separated on polyacrylamide gels. Biotechniques 17:915–919

    Google Scholar 

  • Shen YJ, Jiang H, Jin JP, Zhang ZB, Xi B, He YY, Wang G, Wang Chen, Qian LL, Li X, Yu QB, Liu HJ, Chen DH, Gao JH, Huang H, Shi TL, Yang ZN (2004) Development of genome wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol 135:1198–1205

    Article  PubMed  CAS  Google Scholar 

  • Temnykh S, Park WD, Ayres N, Cartinour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch SR (2000) Mapping and genome organization of microsatellite sequence in rice (Oryza sativa L.). Theor Appl Genet 100:697–712

    Article  CAS  Google Scholar 

  • Wan J, Ikehashi H (1995) Identification of a new locus S-16 causing hybrid sterility in native rice varieties (Oryza sativa L.) from Tai-hu Lake region and Yunnan province, China. Breed Sci 45:161–170

    Google Scholar 

  • Wan J, Yanagihara S, Kato H, Ikehashi H (1993) Multiple alleles at a new locus causing hybrid sterility between a Korean indica variety and japonica variety in rice. Jpn J Breed 43:507–516

    Google Scholar 

  • Wan J, Yamaguchi Y, Kato H, Ikehashi H (1996) Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.). Theor Appl Genet 92:183–190

    Article  CAS  Google Scholar 

  • Wang GW, He YQ, Xu CG, Zhang QF (2006) Fine mapping of f5-Du, a gene conferring wide-compatibility for pollen fertility in inter-subspecific hybrids of rice (Oryza sativa L.). Theor Appl Genet 112:382–387

    Article  PubMed  CAS  Google Scholar 

  • Yanagihara S, Kato H, Ikehashi H (1992) A new locus for multiple alleles causing hybrid sterility between an aus variety and javanica varieties in rice (Oryza sativa L.). Jpn J Breed 42:793–801

    Google Scholar 

  • Yano M, Sasaki T (1997) Genetic and molecular dissection of quantitative traits in rice. Plant Mol Biol 35:145–153

    Article  PubMed  CAS  Google Scholar 

  • Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Bala T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2483

    Article  PubMed  CAS  Google Scholar 

  • Yu CY, Wan JM, Zhai HQ, Wang CM, Jiang L, Xiao YH, Liu YQ (2005) Study on heterosis of inter-subspecies between indica and japonica rice (Oryza sativa L.) using chromosome segment substitution lines. Chin Sci Bull 50:131–136

    Article  Google Scholar 

  • Yuan LP (1992) The strategy of the development of hybrid rice breeding. In: Yuan LP (ed) Current status of two line hybrid rice research, 1–5. Agricultural Publishing Ltd, Beijing (in Chinese, English summary)

    Google Scholar 

  • Zhang GQ, Lu YG, Zhang H, Yang JC, Liu GF (1994a) Genetic studies on the hybrid sterility in cultivated rice (Oryza sativa L.) IV. Genotypes for F1 pollen sterility (in Chinese, English abstract). Acta Genet Sin 21:34–41

    CAS  Google Scholar 

  • Zhang QF, Shen BZ, Dai XK, Mei MH, Maroof MAS, Li ZB (1994b) Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci USA 91:8675–8679

    Article  PubMed  CAS  Google Scholar 

  • Zhang QF, Liu KD, Yang GP, Saghai MA, Xu CG, Zhou ZQ (1997) Molecular marker diversity and hybrid sterility in indica-japonica rice crosses. Theor Appl Genet 95:112–118

    Article  CAS  Google Scholar 

  • Zhao ZG, Wang CM, Jiang L, Zhu SS, Ikehashi H, Wan JM (2006) Identification of a new hybrid sterility gene in rice (Oryza sativa L.). Euphytica 151:331–337

    Article  CAS  Google Scholar 

  • Zhu SS, Wang CM, Zheng TQ, Zhao ZG, Ikehashi H, Wan JM (2005a) A new gene located on chromosome 2 causing hybrid sterility in a remote cross of rice. Plant Breed 124:1–6

    Article  Google Scholar 

  • Zhu SS, Jiang L, Wang CM, Zhai HQ, Li DT, Wan JM (2005b) The origin of a weedy rice Ludao in China deduced by a genome wide analysis of its hybrid sterility genes. Breed Sci 55:409–414

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. A. Yoshimura, Kyushu University, Japan, for kindly providing us with the CSSLs and associated genotypic data. We thank Dr. Gu FL for his help in field management. We thank Dr. Wang JK and Dr. Su N for their help in revising the manuscript. This work was supported by the “863” program of China (No. 2006AA10A101, 2006AA10Z1A5 and 2006AA100101 ), the “973” project (2006CB101706, 2006CB100200), the National Natural Science Foundation of China (No. 30671275), High Technology Program of Jiangsu province (No. BG2004303 and BG2006301) and PCSIRT.

Author information

Authors and Affiliations

  1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China

    Z. G. Zhao, L. Jiang, W. W. Zhang, C. Y. Yu, S. S. Zhu, K. Xie, H. Tian, L. L. Liu & J. M. Wan

  2. Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Zhongguancun South Street 12, Beijing , 100081, China

    J. M. Wan

  3. Department of Breeding, Nihon University, 1866 Kameino, Fujisawa-shi, 2528510, Japan

    H. Ikehashi

Authors
  1. Z. G. Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. W. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Y. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. S. Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. K. Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. H. Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. L. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. H. Ikehashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. J. M. Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. M. Wan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, Z.G., Jiang, L., Zhang, W.W. et al. Fine mapping of S31, a gene responsible for hybrid embryo-sac abortion in rice (Oryza sativa L.). Planta 226, 1087–1096 (2007). https://doi.org/10.1007/s00425-007-0553-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00425-007-0553-8

Keywords

Search

Navigation