Summary
Genetics of four in vitro characters was studied using immature inflorescence-derived callus from four inbred lines. The number of genes controlling total callus quantity varied from 5–11, those for embryogenic(E) callus quantity from 4–7, for callus growth rate from 3–12 and those for regeneration frequency varied from 4–9 in the three segregating F2 populations. Callus derived from the dwarf plants (d2 d2) was phenotypically distinguishable from that of the tall accessions. The association between d2 locus and the in vitro characters was analysed using three different approaches. It is suggested that d2 locus might be closely linked to majority of the loci governing E callus production.
Similar content being viewed by others
References
Aslam, F.N., M.V. Mac Donald, P. Loudon & D.S. Ingram, 1990. Rapid-cycling Brassica speices: inbreeding and selection of B. campestris for anther culture ability. Annal Bot 65: 557–566.
Baroncelli, S., M. Buiatti, A. Bennici & M. Pagliai, 1974. Genetics of growth and differentiation in vitro of Brassica oleracea var. botrytis. III. Genetic correlations and ontogenetic unity. Z Pflanzenzuchtg 72: 275–282.
Bingham, E. T., R.V. Hurley, D.MM. Kaatz & W. Saunders, 1975. Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci 15: 719–721.
Buiatti, M., S. Baroncelli, A. Bennici, M. Pagliai & R. Tesi, 1974. Genetics of growth and differentiation in vitro of Brassica oleracea var. botrytis. II. An in vitro and in vivo analysis of a diallel cross. Z Pflanzenzuchtg 72: 269–274.
Burton, G.W. & J.C. Fortson, 1966. Inheritance and utilization of five dwarfs in pearl millet (Pennisetum typhoides) breeding. Crop Sci 6: 69–72.
Charmet, G. & S. Bernard, 1984. Diallel analysis of androgenetic plant production in hexaploid Triticale (X. triticosecale Wittmack). Theor Appl Genet 69: 55–61.
Chevrier, N., J.A. Qureshi, P. Hucl & K.K. Kartha, 1990. Heritability of in vitro regeneration in wheat (Triticum aestivum L.). Can J Plant Sci 70: 547–550.
Foroughi-Wehr, B., W. Friedt & G. Wenzel, 1982. On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62: 233–239.
Gawell, N.J. & C.D. Robacker, 1990. Genetic control of somatic embryogenesis in cotton petiole callus culture. Euphytica 49: 249–253.
Henry, Y., P. Vain & J. De Buyser, 1994. Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79: 45–58.
Komatsuda, T., S. Enomoto & K. Nakajima, 1989. Genetics of callus proliferation and shoot differentiation in barley. J Hered 80: 345–350.
Lange, C.E., L.C. Federizzi, F.I.F. Carvalho, M.J.C.M.S. Tavares, A.L.C. Dornelles & C.L. Handel, 1995. Genetic analysis of somatic embryogenesis and plant regeneration of wheat (Triticum aestivum L.). J Genet & Breed 49: 195–200.
Lazar, M.D., P.S. Baenziger & G.W. Schaeffer, 1984. Combining abilities and heritabiity of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures. Theor Appl Genet 68:131–134.
Mather, K. & J.L. Jinks, 1982. Biometrical Genetics, 3rd Edition, Chapman and Hall, Lndon.
Mathias, R.J. & E. Atkinson, 1988. In vitro expression of genes affecting whole plant phenotype- the effect of Rht/ Gai alleles on the callus culture response of wheat (Triticum aestivum L.em.Thell.). Theor Appl Genet 75: 474–479.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Mythili, P.K., V. Satyavathi, G. Pavan Kumar, M.V.S. Rao & V. Manga, 1997. Genetic analysis of short term callus culture and morphogenesis in pearl millet, Pennisetum glaucum. Plant Cell Tiss Org Cult 50: 171–178.
Nabors, M.W., J.W. Heyser, T.A. Dykes & K.J. Demott, 1983. Long-duration, high frequency plant regeneration from cereal tissue cultures. Planta 157: 139–142.
Nadolska-Orczyk, A. & S. Malepszy, 1989. In vitro culture of Cucumis sativus L. 7. Genes controlling plant regeneratiton. Theor Appl Genet 78: 836–840.
Petolino, J.F. & S.A. Thompson, 1987. Genetic analysis of anther culture response in maize. Theor Appl Genet 74: 284–286.
Rakoczy-Trojanowska, M. & S. Malepszy, 1993. Genetic factors influencing regeneration ability in rye (Secale cereale L.) immature inflorescences. Theor Appl Genet 86: 406–410.
Rangan, T.S., 1976. Growth and plantlet regeneration in tissue cultures of some Indian millets: Paspalum scrobiculatum L., Eleusine coracana Gaertner and Pennisetum typhoideum Z Pflanzenphysil 78: 208–216.
Reisch, B. & E.T. Bingham, 1980. The genetic control of bud formation from callus cultures of diploid alfalfa. Plant Sci Lett 20: 71–77.
Snedecor, G.W. & W.G. Cochran, 1968. Statistical methods. The Iowa State University Press, Ames, Iowa, USA.
Subba Rao, M.V. & W. Nitzsche, 1984. Genotypic differences in callus growth and organogenesis of eight pearl millet lines. Euphytica 33: 923–928.
Talwar, M. & A. Rashid, 1990. Factors effecting formation of somatic embryos and embyogenic callus from unemerged inflorescence of a graminaceous crop, Pennisetum. Ann Bot 66: 17–21.
Vasil, V. & I.K. Vasil, 1981. Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum and P.americanum × P. purpureum hybrid. Amer J Bot 68: 864–872.
Vasil, V. & I.K. Vasil, 1982. The ontogeny of somatic embryos of Pennisetum americanum (L.) K. Schum. 1. In cultured immature embryos. Bot Gaz 143: 454–465.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Satyavathi, V., Rao, M.V.S., Manga, V. et al. Genetics of some in vitro characters in pearl millet. Euphytica 148, 243–249 (2006). https://doi.org/10.1007/s10681-005-9015-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-9015-1