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Enhanced cell attachment using a novel cell culture surface presenting functional domains from extracellular matrix proteins

  • Method in Cell Science
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Abstract

Many factors contribute to the creation and maintenance of a realistic environment for cell growth in vitro, e.g. the consistency of the growth medium, the addition of supplements, and the surface on which the cells grow. The nature of the surface on which cells are cultured plays an important role in their ability to attach, proliferate, migrate and function. Components of the extracellular matrix (ECM) are often used to coat glass or plastic surfaces to enhance cell attachment in vitro. Fragments of ECM molecules can be immobilised on surfaces in order to mimic the effects seen by whole molecules. In this study we evaluate the application of a novel technology for the immobilisation of functional domains of known ECM proteins in a controlled manner on a surface. By examining the adherence of cultured PC12 cells to alternative growth surfaces, we show that surfaces coated with motifs from collagen I, collagen IV, fibronectin and laminin can mimic surfaces coated with the corresponding whole molecules. Furthermore, we show that the adherence of cells can be controlled by modifying the hydropathic properties of the surface to either enhance or inhibit cell attachment. Collectively, these data demonstrate the application of a new technology to enable optimisation of cell growth in the tissue culture laboratory.

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Acknowledgements

This work was supported by Orla Protein Technologies Ltd. (http://www.orlaproteins.com) and The Biotechnology and Biological Sciences Research Council (BBSRC, http://www.bbsrc.ac.uk).

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Authors and Affiliations

  1. North East England Stem Cell Institute (NESCI), School of Biological and Biomedical Science, University of Durham, South Road, Durham, DH1 3LE, UK

    M. J. Cooke & S. A. Przyborski

  2. Orla Protein Technologies Ltd., Nanotechnology Centre, Herschel Building, Newcastle Upon Tyne, NE1 7RU, UK

    S. R. Phillips, D. S. H. Shah, D. Athey & J. H. Lakey

  3. Institute for Cell and Molecular Biosciences, University of Newcastle Upon Tyne, Newcastle Upon Tyne, NE2 4HH, UK

    J. H. Lakey

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  1. M. J. Cooke
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  2. S. R. Phillips
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  3. D. S. H. Shah
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  4. D. Athey
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  5. J. H. Lakey
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  6. S. A. Przyborski
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Correspondence to S. A. Przyborski.

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Cooke, M.J., Phillips, S.R., Shah, D.S.H. et al. Enhanced cell attachment using a novel cell culture surface presenting functional domains from extracellular matrix proteins. Cytotechnology 56, 71–79 (2008). https://doi.org/10.1007/s10616-007-9119-7

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  • DOI: https://doi.org/10.1007/s10616-007-9119-7

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