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Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells

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Abstract

Ectoderm-derived mesenchymal stem cells (EMSCs) were used as potential seed cells for bone tissue engineering to treat bone defects due to their capability of rapid proliferation and osteogenic differentiation. Sonic hedgehog (Shh) signaling was reported to play an important role in the development of bone tissue, but its role is not understood. The present study investigated the role of Shh molecule in osteogenic differentiation of rat EMSCs in vitro. Rat EMSCs were isolated form nasal respiratory mucosa and identified with immunofluorescence and analyzed with other methods, including reverse transcriptase polymerase chain reaction (qPCR) and western blotting. EMSCs expressed CD90, CD105, nestin, and vimentin. On the seventh day of osteogenic induction, expression levels of Shh and Gli1 was higher according to the result of qPCR and Western blotting. After induction for 14 days, higher alkaline phosphatase (ALP) activity and more mineralized nodules were seen in comparison to the cells that did not undergo induction. Shh signaling appears to enhance osteogenic differentiation of rat EMSCs, suggesting that Shh signaling directs the lineage differentiation of ectodermal stem cells and represents a promising strategy for skeletal tissue regeneration.

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Acknowledgements

The authors also thank the University Ethics Committee for their kind guidance in the animal experiments. I would like to thank all my friends, especially my four lovely teammates, for their encouragement and support.

Funding

The present study was supported by grants from the National Natural Science Foundation of China (Granted Numbers: 81671541, 81273202, 81701545, and 82071738), and by the Graduate Innovation Program of Jiangsu University (Granted Number: CXLX12_0675).

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Author notes

  1. Weijiang Wu

    Present address: Department of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China

Authors and Affiliations

  1. Department of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Jili Xu, Zhiqiang Huang & Qixiang Shao

  2. Department of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, 212001, Jiangsu, People’s Republic of China

    Weijiang Wu, Zhe Wang, Zhijian Zhang & Wenjing Yang

  3. Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, Jiangsu, People’s Republic of China

    Xin Fan

  4. School of Medical Science and Laboratory Medicine, Jiangsu College of Nursing, Huai’an, 223002, Jiangsu, People’s Republic of China

    Qixiang Shao

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  1. Weijiang Wu
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Weijiang Wu and Zhe Wang contribute equally to this work.

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Correspondence to Qixiang Shao.

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Wu, W., Wang, Z., Zhang, Z. et al. Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells. Cell Tissue Bank 23, 569–580 (2022). https://doi.org/10.1007/s10561-022-09994-4

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