Abstract
The aim of this study is to identify rat nasal septum respiratory mucosa-derived mesenchyme stem cells (RM-MSCs) and to compare its neural lineage differentiation capacity with bone marrow-derived mesenchyme stem cells (BM-MSCs) after a short period of neural induction culture in vitro. The cell morphology was observed with light microscopy; cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The characteristics of the cells were evaluated with flow cytometry, immunofluorescence, real-time quantitative PCR (RT-PCR), and Western blotting. The results showed that rat nasal respiratory mucosa contains RM-MSCs that exhibited similar proliferation rate as BM-MSCs in vitro. Both RT-PCR and Western blotting analyses demonstrated that RM-MSCs showed higher expression of neural lineage markers than BM-MSCs after a short period of neural induction culture, and secreted higher level of brain-derived neurotrophic factor. RM-MSCs were more amenable to differentiate into neural or glial cell after a short period of neural induction culture than BM-MSCs in vitro; and it could be considered as another optimal source of stem cells for cell-based therapy to neurological diseases.
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Acknowledgments
This study was supported by grants from Jiangsu Province Health Department (KF200956) and the Changzhou Science & Technology Bureau (CJ20122027), P. R. China. The authors acknowledge Dr. Jizong Gao, MD, PhD, for his critical editorial assistance. We are also gratitude to an anonymous reviewer for his/her professional comments and suggestions during the reviewing processes.
Conflict of interest
We state that the manuscript, or parts of it, have not been and will not be submitted elsewhere for publication. Xin Gao, Jinbo Liu, Jian Zhang, Jun Zhang, and Hongjun Zou have agreed to the submission of the final manuscript and declare no conflicts of interest.
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Gao, X., Zhang, J., Zhang, J. et al. Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro. Cell Mol Neurobiol 34, 257–268 (2014). https://doi.org/10.1007/s10571-013-0009-6
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DOI: https://doi.org/10.1007/s10571-013-0009-6