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The B-, G- and S-genomic Chi genes in family Triticeae

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Biologia Plantarum

Abstract

As result of a close evolutionary relationship between Triticeae B, G, and S genomes, the exchange of genetic material between them is possible and may be beneficial for broadening the genetic diversity of cultivated bread wheat. However, the extent to which regulatory networks are conserved remains poorly researched. Here, the structural organization and transcriptional activity of the B, S, and G genome copies of a gene encoding flavonoid biosynthesis enzyme chalcone-flavanone isomerase (CHI) were explored using introgression lines which differ from the wild type by carrying a non-bread wheat Chi-1 gene. Chi-S1, Chi-G1, and Chi-B1 all mapped to a comparable region of chromosomes 5S, 5G, and 5B, respectively. Nucleotide sequences of Aegilops speltoides Chi-S1 and Triticum timopheevii Chi-G1 were determined and compared with T. aestivum Chi-B1 sequences. The enzymes encoded by these three genes shared the same predicted tertiary structure and active sites. However, the replacement of Chi-B1 by Chi-S1 or Chi-G1 in a wheat background resulted in a significant decrease in the global amount of the Chi-1 transcript present in the seedling shoot indicating divergence in regulation of expression of the orthologous Chi-1 genes among Triticeae ssp.

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Abbreviations

CHI:

chalcone-flavanone isomerase

Chi :

gene encoding chalcone-flavanone isomerase

Ka :

number of non-synonymous substitutions

Ks :

number of synonymous substitutions

RT-qPCR:

reverse transcription quantitative polymerase chain reaction

Ubc :

gene encoding ubiquitin

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia

    O. Y. Shoeva, O. B. Dobrovolskaya, I. N. Leonova, E. A. Salina & E. K. Khlestkina

  2. Food Security Research Center, Novosibirsk State University, Novosibirsk, 630090, Russia

    E. K. Khlestkina

Authors
  1. O. Y. Shoeva
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  2. O. B. Dobrovolskaya
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  3. I. N. Leonova
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  4. E. A. Salina
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  5. E. K. Khlestkina
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Corresponding author

Correspondence to O. Y. Shoeva.

Additional information

Acknowledgements: This study was partially supported by the State Budget Programme (Project No VI.53.1.5. = 0324-2015-0005), the Siberian Branch of the Russian Academy of Science (Integration project SBRAS/NAS Belarus No 22) and RFBR (Grant No 16-34-60052).We thank Ms. Galina Generalova for technical assistance and www.smartenglish.co.uk for linguistic advice in the preparation of the first manuscript draft.

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Shoeva, O.Y., Dobrovolskaya, O.B., Leonova, I.N. et al. The B-, G- and S-genomic Chi genes in family Triticeae . Biol Plant 60, 279–284 (2016). https://doi.org/10.1007/s10535-016-0595-5

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  • DOI: https://doi.org/10.1007/s10535-016-0595-5

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