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Analysis of differentially expressed genes in response to endogenous cytokinins during cotton leaf senescence

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Biologia Plantarum

Abstract

Cytokinins have been implicated in delaying leaf senescence. We previously generated transgenic cotton (Gossypium hirsutum L.) plants that harbor the Agrobacterium isopentenyl transferase gene (ipt) directed by a proteinase gene promoter. Here, we report that mRNAs were isolated from ipt cotton leaves and azygous leaves and were subsequently sequenced using Illumina Solexa technology. The sequence tags were searched against the TIGR database and the related gene expression profiles were compared resulting in the identification of 1 218 differentially expressed genes (DEGs): 719 up-regulated and 499 down-regulated. Analyzing the DEGs in the ipt cotton leaves showed that these genes belonged to four pathways: flavone biosynthesis, arginine and proline metabolism, glyoxylate and dicarboxylate metabolism, and RNA degradation. These pathways increased the activities of antioxidants, inhibited the effect of ethylene, and prevented degradation of macromolecules during senescence. The expression patterns of 17 genes were evaluated by real-time PCR and results were in agreement with the patterns of sequencing analysis. The identification of the DEGs may help us to understand a role of cytokinins in leaf senescence.

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Abbreviations

AHK:

Arabidopsis histidine kinase

DAE:

days after emergence

DEG:

differentially expressed gene

IPT:

isopentenyl transferase

ODC:

ornithine decarboxylase

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, Shandong, P.R. China

    P. Zhao, N. Zhang, Z. J. Yin, Y. D. Liu & F. F. Shen

  2. State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, 455100, Henan, P.R. China

    Z. J. Yin

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  1. P. Zhao
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  2. N. Zhang
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  3. Z. J. Yin
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  4. Y. D. Liu
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  5. F. F. Shen
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Correspondence to F. F. Shen.

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Acknowledgements: This research was mainly supported by the China Major Projects for Transgenic Breeding (Grant Nos. 2011ZX005-004 and 2011ZX08005-002) and the China Key Development Project for Basic Research (973) (Grant No. 2010CB12600).

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Zhao, P., Zhang, N., Yin, Z.J. et al. Analysis of differentially expressed genes in response to endogenous cytokinins during cotton leaf senescence. Biol Plant 57, 425–432 (2013). https://doi.org/10.1007/s10535-013-0324-2

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  • DOI: https://doi.org/10.1007/s10535-013-0324-2

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