Abstract
Soil salinity is a major abiotic stress and salt overly sensitive (SOS) pathway plays an important role in imparting tolerance to salinity by reinstating cellular ionic equilibrium. Salt overly sensitive 1 (SOS1) gene of SOS pathway has been implicated in increasing salt tolerance in plants. In this study, a 734 bp fragment of SOS1 promoter (SbUSOS1) was isolated from a halophyte Salicornia brachiata Roxb. In silico analysis of SbUSOS1 predicted several cis-acting regulatory elements such as DOF motif, GT elements, ABRE-like sequence, and root specific motifs. Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold (4 °C) stresses. This study indicated that SbUSOS1 was functional with predicted cis-acting elements that could be responsible for its salt-inducible nature. It can be used for the development of salt stress tolerant transgenic plants.
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Abbreviations
- AtUSOS1:
-
Arabidopsis thaliana SOS1 gene upstream sequence
- CaM:
-
calmodulin
- EDTA:
-
ethylene-diamine tetraacetate
- EtBr:
-
ethidium bromide
- GUS:
-
β-glucuronidase
- LB:
-
Luria broth
- MEME:
-
multiple expectation maximization for motif elicitation
- MES:
-
2-(N-morpholino) ethanesulfonic acid
- PLACE:
-
plant cis-acting regulatory DNA elements
- SbUSOS1:
-
Salicornia brachiata upstream sequence of SOS1 gene
- ThUSOS1:
-
Thellungiella halophila SOS1 gene upstream sequence
- X-gluc:
-
5-bromo-4-chloro-3-indolyl-β-d-glucuronide
- YEMA:
-
yeast extract mannitol agar
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Acknowledgements: This financial support received from Department of Biotechnology, New Delhi under the project entitled “Development of transgenic groundnut (Arachis hypogaea L.) for enhanced abiotic stress tolerance” is thankfully acknowledged. The authors are grateful to the Director, National Research center on Plant Biotechnology, NRCPB, New Delhi and Dr. Bhavnath Jha, CSMCRI, Bhavnagar for providing seeds of S. brachiata and valuable suggestions.
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Goyal, E., Singh, R.S. & Kanika, K. Isolation and functional characterization of Salt overly sensitive 1 (SOS1) gene promoter from Salicornia brachiata . Biol Plant 57, 465–473 (2013). https://doi.org/10.1007/s10535-013-0309-1
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DOI: https://doi.org/10.1007/s10535-013-0309-1