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Adventitious rooting of detached Arabidopsis thaliana leaves

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Biologia Plantarum

Abstract

Arabidopsis thaliana Heyhn is a model species in biochemical, physiological and molecular studies for which a plethora of mutants is available. This work aimed at developing a system for rooting of detached leaves, and evaluating time course of several relevant biochemical parameters during rooting assays with and without auxins. The rooting pattern was of the direct type (without callus formation) in all of the treatments and ecotypes analyzed and was rather stable. Considering the different parameters examined, peroxidase activity and contents of phenolic compounds and soluble sugars appeared as the most distinct biochemical markers of the rooting process in this system.

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Abbreviations

ABRC:

Arabidopsis Biological Resource Center

AFR:

adventitious root formation

An:

ecotype Antwerpeen

Chl:

chlorophyll

Col:

ecotype Columbia

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

NAA:

naphthaleneacetic acid

POX:

peroxidase

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Acknowledgements

This work was supported by the Brazilian Funding agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Comissão de Aperfeiçoamento de Pessoal de NÍvel Superior (CAPES).

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Authors and Affiliations

  1. Departamento de Botânica, Laboratório de Fisiologia Vegetal, Universidade Federal do Rio Grande do Sul, CP 15005, CEP 91501-970, Porto Alegre, RS, Brazil

    L. R. Correa & A. G. Fett-Neto

  2. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, CP 15005, CEP 91501-970, Porto Alegre, RS, Brazil

    R. J. Stein & A. G. Fett-Neto

Authors
  1. L. R. Correa
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  2. R. J. Stein
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  3. A. G. Fett-Neto
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Correspondence to A. G. Fett-Neto.

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Correa, L.R., Stein, R.J. & Fett-Neto, A.G. Adventitious rooting of detached Arabidopsis thaliana leaves. Biol Plant 56, 25–30 (2012). https://doi.org/10.1007/s10535-012-0011-8

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  • DOI: https://doi.org/10.1007/s10535-012-0011-8

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