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Micropropagation of Harpagophytum procumbens

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

An efficient protocol for micropropagation of Harpagophytum procumbens DC., an endangered African medicinal plant, was developed. Maximum shoot multiplication without callus was obtained from nodal explants cultured on Murashige and Skoog (MS) basal salts plus Gamborg’s (B5) vitamins supplemented with 0.1 mg dm−3 indole-3-acetic acid and 5.0 mg dm−3 kinetin. The shoots were subsequently subcultured every 3 weeks on the same medium. Detached axillary shoots were transferred to MS basal salts plus B5 vitamins supplemented with various concentrations of α-naphthalene-acetic acid or indole-3-butyric acid (IBA), ranging from 0.5 to 2.5 mg dm−3 and 100 % rooting and optimal subsequent acclimatization was achieved on 1.0 mg dm−3 IBA. After 4 weeks of culture, the rooted shoots (>5 cm) were planted in pots containing peat, vermiculite and bark (2:1:1), covered with plastic domes and maintained at 25 °C for 2 weeks before being transferred to a glasshouse. Plant survival was about 40 %.

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Abbreviations

ADE:

adenine

AH:

adenine hemisulphate

BAP:

6-benzylaminopurine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

kinetin

MS:

Murashige and Skoog

NAA:

α-naphthalene acetic acid

PGR:

plant growth regulator

Z:

zeatin

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Author notes

  1. S. Kaliamoorthy

    Present address: Botanical Survey of India, Southern Circle, TNAU Campus, Lawley Road Post, Coimbatore-641003, Tamil Nadu, India

Authors and Affiliations

  1. School of Biological and Conservation Sciences, University of KwaZulu-Natal, P/B X 54001, Durban 0]4000, South Africa

    S. Kaliamoorthy, G. Naidoo & P. Achar

Authors
  1. S. Kaliamoorthy
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  2. G. Naidoo
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  3. P. Achar
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Correspondence to S. Kaliamoorthy.

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Kaliamoorthy, S., Naidoo, G. & Achar, P. Micropropagation of Harpagophytum procumbens . Biol Plant 52, 191–194 (2008). https://doi.org/10.1007/s10535-008-0043-2

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  • DOI: https://doi.org/10.1007/s10535-008-0043-2

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