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An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers

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  • Published:
Biologia Plantarum

Abstract

Rapid micropropagation was achieved in Chlorophytum borivilianum Santapau and Fernandes using shoot base as explants. Multiple shoots were induced on Murashige and Skoog’s (MS) medium supplemented with 3.0 mg dm−3 6-benzylaminopurine, 0.1 mg dm−3 1-naphthaleneacetic acid, 150 mg dm−3 adenine sulphates and 3 % saccharose. Rooting was readily achieved upon transferring the shoots onto half strength MS medium supplemented with 0.1 mg dm−3 indolebutyric acid and 2 % saccharose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of the micropropagated plants. Thirty one arbitrary decamers were used to amplify genomic DNA from in vitro and in vivo plant material to assess the genetic stability. All RAPD profile analysis from micropropagated plants was genetically similar to mother plants.

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Abbreviations

Ads:

adenine sulphate

BA:

6-benzylaminopurine

bp:

base pair

IAA:

indole-3-acetic acid

IBA:

indolebutyric acid

Kin:

kinetin

NAA:

1-naphthaleneacetic acid

MS medium:

Murashige and Skoog (1962) medium

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

CTAB:

cetyltrimethyl ammonium bromide

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Acknowledgement

The authors are thankful to Dr. K.A. Geetha, for providing her registered material for the study and the Indian Council of Agricultural Research (ICAR) for funding support in the form of granting CESS fund adhoc project.

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  1. Directorate of Medicinal and Aromatic Plants Research, Boriavi, Anand, 387310, Gujarat, India

    S. Samantaray & S. Maiti

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  1. S. Samantaray
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  2. S. Maiti
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Correspondence to S. Samantaray.

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Samantaray, S., Maiti, S. An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers. Biol Plant 54, 334–338 (2010). https://doi.org/10.1007/s10535-010-0058-3

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  • DOI: https://doi.org/10.1007/s10535-010-0058-3

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