Abstract
In vitro clonal multiplication of safed musli (Chlorophytum borivilianum Sant. et. Fernand.), a rare Indian medicinal herb, has been achieved on Murashige and Skoog's (MS) medium supplemented with 22.2 μM benzyladenine using young shoot bases as explants. Shoots multiplied at a rate of four-fold every 3 weeks. All shoots rooted when transferred to MS medium with 3/4-strength inorganic and organic constituents and 9.8 μM indolebutyric acid and 67% of the micropropagated plants were successfully established in pots. Such plants produced normal fasciculated storage roots as in wild plants.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- MS:
-
Murashige and Skoog (1962)
- NAA:
-
α-naphthaleneacetic acid
References
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Purohit, S.D., Dave, A. & Kukda, G. Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tiss Organ Cult 39, 93–96 (1994). https://doi.org/10.1007/BF00037596
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DOI: https://doi.org/10.1007/BF00037596