Abstract
A cytological and molecular analysis was performed to assess the genetic uniformity and true-to-type character of plants regenerated from 20 week-old embryogenic suspension cultures of meadow fescue (Festuca pratensis Huds.), and compared to protoplastderived plants obtained from the same cell suspension. Cytological variation was not observed in a representative sample of plants regenerated directly from the embryogenic suspensions and from protoplasts isolated therefrom. Similarly, no restriction fragment length polymorphisms (RFLPs) were detected in the mitochondrial, plastid and nuclear genomes in the plants analyzed. Randomly amplified polymorphic DNA markers (RAPDs) have been used to characterise molecularly a set of mature meadow fescue plants regenerated from these in vitro cultures. RAPD markers using 18 different short oligonucleotide primers of arbitrary nucleotide sequence in combination with polymerase chain reaction (PCR) allowed the detection of pre-existing polymorphisms in the donor genotypes, but failed to reveal newly generated variation in the protoplast-derived plants compared to their equivalent suspensionculture regenerated materials.
The genetic stability of meadow fescue plants regenerated from suspension cultures and protoplasts isolated therefrom and its implications on gene transfer technology for this species are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- RFLP:
-
restriction fragment length polymorphism.
References
Aastveit AH, Aastveit K (1989) Hereditas 111:103–114
Baird E, Cooper-Bland S, Waugh R, DeMaine M, Powell W (1992) Mol. Gen. Genet. 233:469–475
Chandler SF, Vasil IK (1984) J. Plant Physiol. 117:147–156
Evans DA (1989) Trends in Genetics 5:46–50
Feinberg AP, Vogelstein B (1983) Anal. Biochem. 132:6–13
Haydu Z, Vasil IK (1981) Theor. Appl. Genet. 59:269–273
Hulten E (1971) Atlas of the distribution of vascular plants in northwestern Europe. Generalstabens Litografiska Anstaltsförlag, Stockholm
Humphreys MW, Dalton SJ (1991) Genome 34:59–65
Humphreys MW, Dalton SJ (1992) Genome 35:461–467
Lichtenstein C, Draper J (1985) Genetic engineering of plants. In: Glover, D.M. (ed) DNA cloning, vol II, IRL Press, Oxford, Washington, 67–119
Pérez-Vicente R, Petris L, Osusky M, Potrykus I, Spangenberg G (1992) Theor. Appl. Genet. 84:145–154
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning — a laboratory manual. Second edition. Cold Spring Harbor Laboratory Press, New York
Shenoy VB, Vasil IK (1992) Theor. Appl. Genet. 83:947–955
Swedlund BD, Vasil IK (1985) Theor. Appl. Genet. 69:575–581
Vasil V, Vasil IK (1981) Am. J. Bot. 68:864–872
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) Nucleic Acids Res. 18:6531–6535
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Vallés, M.P., Wang, Z.Y., Montavon, P. et al. Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue (Festuca pratensis Huds.). Plant Cell Reports 12, 101–106 (1993). https://doi.org/10.1007/BF00241943
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00241943