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The detection of somaclonal variants of beet using RAPD

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Summary

Plantlets were regenerated by adventitious shoot budding in tissue culture from leaf explants of a single genotype of sugar beet. DNA was extracted from the parental plant and from 120 regenerants. RAPD analysis was carried out using five decanucleotide primers; 4,557 RAPD marker bands were examined and two polymorphisms were observed. Thirty secondary regenerants were then derived, using the same tissue culture technique, from thirty of the primary regenerants. Again RAPD analysis was employed and a single band polymorphism was observed out of 1,050 bands examined. The overall frequency of detection of somaclonal polymorphisms using RAPD (3 in 5,607 = 0.05%) is similar to frequencies previously reported using isozyme and RFLP technologies.

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Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole-butyric acid

MS:

Murashige and Skoog medium (Murashige and Skoog, 1962)

RAPD:

Random Amplification of Polymorphic DNA

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Authors and Affiliations

  1. School of Biological Sciences, University of Birmingham, PO Box 363, B15 2TT, Birmingham, UK

    H. J. Newbury & B. V. Ford-Lloyd

  2. Dipartimento di Genetica e di Biologia dei Microorganismi, Via Celoria 26, 20133, Milan, Italy

    M. T. Munthali

Authors
  1. M. T. Munthali
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  2. H. J. Newbury
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  3. B. V. Ford-Lloyd
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Communicated by P. J. King

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Munthali, M.T., Newbury, H.J. & Ford-Lloyd, B.V. The detection of somaclonal variants of beet using RAPD. Plant Cell Reports 15, 474–478 (1996). https://doi.org/10.1007/BF00232977

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  • DOI: https://doi.org/10.1007/BF00232977

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