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Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Plantago algarbiensis and P. almogravensis are endemic Al tolerant species from the Western-centre of the Algarve region (South of Portugal) and Portuguese Southwest coast, respectively, which are in risk of global extinction. The aim of this work was to establish an efficient protocol to in vitro propagate these species using shoots obtained from in vitro germinated seeds. The best results in terms of multiplication response were afforded in Murashige and Skoog’s (MS) medium supplemented with 6-benzyladenine (8.5 and 9.2 shoots per explant in P. algarbiensis and P. almogravensis, respectively). Shoots of both species showed a great rooting capacity (100 and 80 % for P. algarbiensis and P. almogravensis, respectively) that was not significantly influenced by the concentration of MS macronutrients or auxins. Plants were acclimatized to ex vitro conditions, exhibited normal development (survival rate of 95 and 80 % in P. algarbiensis and P. almogravensis, respectively), and were successfully reintroduced in their natural habitat.

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Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KIN:

kinetin

MS:

Murashige and Skoog medium

ZEA:

zeatin

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Authors and Affiliations

  1. Faculty of Sciences and Technology, University of Algarve, and Institute for Biotechnology and Bioengineering, Campus de Gambelas, 8005-139 Faro, and IBB/CGB-UTAD, 5001-801, Vila Real, Portugal

    S. Gonçalves, N. Martins & A. Romano

Authors
  1. S. Gonçalves
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  2. N. Martins
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  3. A. Romano
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Corresponding author

Correspondence to A. Romano.

Additional information

Acknowledgments: S. Gonçalves and N. Martins acknowledge a grant from Portuguese Science and Technology Foundation (FCT, Grant SFRH/BPD/31534/2006 and SFRH/BD/48379/2008, respectively). This work was partially supported by the project EcoDilema (POCI/BIA-BDE/60664/2004).

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Gonçalves, S., Martins, N. & Romano, A. Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis . Biol Plant 53, 774–778 (2009). https://doi.org/10.1007/s10535-009-0142-8

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  • DOI: https://doi.org/10.1007/s10535-009-0142-8

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