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Micropropagation and salt tolerance of in vitro grown Crithmum maritimum L.

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Abstract

Crithmum maritimum (Apiaceae), a perennial halophyte native in Greece, could be used as an alternative culture at problematic soils. It presents significant economical potentials as its essential oils are in high demand from the medicinal and cosmetic industry. The response of the species on in vitro conditions was studied. MS proved to be the most effective of the basal media tested for in vitro adventitious shoot production, resulting in significantly increased number of new microshoots/explant and higher shoots. 6-Benzyladenine (BA) at 2.5 μM increased shoot proliferation. The combination of α-Naphthaleneacetic acid (NAA) (1–2.5 μM) with BA (2.5 μM) had a positive influence at simultaneous proliferation and rooting resulting in high rooting percentage (82.5–95%) and increased number of roots. Rooting percentage reached 100% and number of roots increased significantly when 0.5 μM and 1 μM IBA was combined with ½MS and full strength MS. The in vitro response to salinity stress (0–300 mM NaCl) was also tested. Shoot proliferation was gradually reduced at higher concentrations of NaCl but shoot height was enhanced. Acclimatization procedure was successful.

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Abbreviations

BA:

6-Benzyladenine

B5:

Gamborg medium

IBA:

Indole-3-butyric acid

NAA:

α-Naphthaleneacetic acid

MS:

Murashige and Skoog medium

WPM:

Woody plant medium

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Acknowledgements

This work has been performed at the framework of the programs: (a) Development of Industrial Research (05ΠAB219) financed by the General Secretary of Research and Technology of Greece and (b) “Exploitation actions for the multi-functionality of Balkan Botanic Garden Kroussia” and “Conservation and exploitation of the Balkan Flora” financed by the Greek Ministry of Economy and Finance.

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Authors and Affiliations

  1. Laboratory of Conservation and Exploitation of Native and Floricultural Species, Agricultural Research Center of Macedonia and Thrace, National Agricultural Research Foundation (NAGREF), P.O. Box 60125, Thermi, Thessaloniki, 57001, Greece

    Katerina Grigoriadou & Eleni Maloupa

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  1. Katerina Grigoriadou
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  2. Eleni Maloupa
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Correspondence to Katerina Grigoriadou.

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Grigoriadou, K., Maloupa, E. Micropropagation and salt tolerance of in vitro grown Crithmum maritimum L.. Plant Cell Tiss Organ Cult 94, 209–217 (2008). https://doi.org/10.1007/s11240-008-9406-9

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