Abstract
Sucrose concentration in the culture medium affected chlorophyll content, trichome development and amount of solasodine in regenerated plantlets of Solanum trilobatum. High chlorophyll content and glandular trichomes were observed in the plants grown on Murashige and Skoog basal medium supplemented with 131.85 mM sucrose. The solasodine was quantified using reverse phase high performance liquid chromatography. The plantlets cultivated on this medium yielded 35.97 mg g−1 (d.m.) solasodine whereas the field plants used as control yielded only 2.32 mg g−1 (d.m.) of solasodine.
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Abbreviations
- Chl:
-
chlorophyll
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- d.m.:
-
dry mass
- GI:
-
growth index
- IAA:
-
indole-3-acetic acid
- KIN:
-
kinetin
- L/W:
-
length/width ratio
- RP-HPLC:
-
reverse phase high pressure liquid chromatography
- SEM:
-
scanning electron microscopy
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Acknowledgements: KA was supported by a Teacher Fellowship Scheme from University Grants Commission, New Delhi, India.
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Anirudhan, K., Nair, A.S. In vitro production of solasodine from Solanum trilobatum . Biol Plant 53, 719–722 (2009). https://doi.org/10.1007/s10535-009-0130-z
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DOI: https://doi.org/10.1007/s10535-009-0130-z