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Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation

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  • Published:
Biologia Plantarum

Abstract

Withanolides-steroidal lactones, isolated from various Solanaceous plants have received considerable attention due to their potential biological activities. Five selected withanolides (withanone, withaferin A, withanolide A, withanolide B, withanolide E) were identified by HPLC-UV (DAD) — positive ion electrospray ionization mass spectroscopy in Withania somnifera (L.) Dunal cv. WSR plants and tissues cultured in vitro at different developmental phases. Cultures were established from five explants on Murashige and Skoog’s medium supplemented with different plant growth regulators. Results suggest that production of withanolides is closely associated with morphological differentiation.

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Abbreviations

B5 :

Gamborg’s (1968) medium

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

HPLC:

high pressure liquid chromatography

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KN:

kinetin

MS medium:

Murashige and Skoog (1962) medium

NAA:

α-naphthalene acetic acid

NMR:

nuclear magnetic resonance

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Regional Research Laboratory, CSIR, Jammu-Tawi, 180001, India

    M. Sharada, A. Ahuja, K. A. Suri, R. K. Khajuria, V. Verma & A. Kumar

  2. Shoolini Institute of Life Sciences & Business Management, Anand Campus, Solan, 173212, HP, India

    S. P. Vij

Authors
  1. M. Sharada
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  2. A. Ahuja
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  3. K. A. Suri
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  4. S. P. Vij
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  5. R. K. Khajuria
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  6. V. Verma
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  7. A. Kumar
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Correspondence to A. Ahuja.

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Sharada, M., Ahuja, A., Suri, K.A. et al. Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation. Biol Plant 51, 161–164 (2007). https://doi.org/10.1007/s10535-007-0031-y

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  • DOI: https://doi.org/10.1007/s10535-007-0031-y

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