Summary
Suspension cells of Taxus brevifolia were cultured as an alternative source of taxol and related taxanes. The effects of basal medium, sugar type, and sugar concentration on growth and taxane production were investigated. To induce taxol production, modification of medium composition was necessary. Fructose supported the best taxol yield and the use of high concentration of sugar was desirable. The maximum level of taxol obtained after 10 days of culture in an optimized condition was 1.43 mg/L, which was 0.013% as a specific content.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Borman, S. (1991). Chem. Eng. News 69, 11–18.
Bringi, V. and Kadkade, P. (1993). WO Patent 9317121.
Fett-Neto, A.G., DiCosmo, F, Reynolds, and Sakata, K. (1992). Bio/Technology 10, 1572–1575.
Fett-Neto, A.G., Melanson, S.J. Sakata, K., and DiCosmo, F. (1993). Bio/Technology 11, 731–734.
Fett-Neto, A.G., Zhang, W.Y., and DiCosmo, F. (1994). Biotechnol. Bioeng. 44, 205–210.
Fujita, Y., Hara, Y., Ogino, T., and Suga, C. (1981). Plant Cell Rep. 1, 59–60.
Knobloch, K-H. and Berlin, J. (1980). Z. Naturforsch. 35c, 551–556.
Mantell, S.H. and Smith, H. (1983). Cultural factors that influence secondary metabolite accumulations in plant cell and tissue cultures. In: Plant Biotechnology, S.H. Mantell and H. Smith, eds., pp. 75–108, Cambridge: Cambridge University Press.
Merillon, J.M., Rideau, M., and Chenieux, J.C. (1984). Planta Med. 50, 497–501.
Panda, A.K., Mishira, S., and Bisaria, V.S. (1992). Biotechnol. Bioeng. 39, 1043–1051.
Park, I.-S. and Kim, D.-I. (1993). Biotechnol. Techniques 7, 627–630.
Park, I.-S., Kim, J.-H., Yun, J.-H., Byun, S.Y., and Kim, D.-I. (1994). In: Better Living Through Innovative Biochemical Engineering (Proceedings of the Third Asia-Pacific Biochemical Engineering Conference), W.K. Teo, M.G.S. Yap, and S.K.W. Oh, eds., pp. 131–133, Singapore: Continental Press.
Tal, B., Gressel, J., and Goldberg, I. (1982). Planta Med. 44, 111–115.
Wani, M.C., Taylor, H.L., Wall, M.E., Coggon, P., and McPhail, A.T. (1971). J. Am. Chem. Soc. 93, 2325–2327.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, JH., Yun, JH., Hwang, YS. et al. Production of taxol and related taxanes in Taxus brevifolia cell cultures: Effect of sugar. Biotechnol Lett 17, 101–106 (1995). https://doi.org/10.1007/BF00134204
Issue Date:
DOI: https://doi.org/10.1007/BF00134204