Skip to main content
SpringerLink
Log in

Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic stability of 80 micropropagated Hagenia abyssinica plants, 40 of axillary origin and 40 of adventitious origin. The shoots were isolated from the same mother tree and micropropagated for over two years. Among the 83 RAPD primers screened, 16 gave reproducible band patterns. These 16 primers produced 115 bands for each plant. One plant from axillary origin showed two unique bands with primer OPC-11. All other plants showed identical band patterns. Generally, there was no significant difference in the shoot multiplication rate between shoots of axillary and adventitious origin. Indole-3-acetic acid (IAA) resulted in better ex vitro rooting compared to indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA). Non-micropropagated plants that were grown in the greenhouse for about one year were better in ex vitro rooting compared to those of juvenile material and mature tree derived micropropagated plants of the same treatment. Adventitious rooting related oxygenase gene (ARRO-1) isolated from apple (Malus domestica) was not expressed in H. abyssinica using a complementary DNA representational difference analysis fragment (cDNA RDA14) as a probe.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2-ODD:

2-oxoacid-dependent dioxygenase

ARRO-1:

adventitious rooting related oxygenase

BAP:

benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog (1962)

NAA:

α-naphthaleneacetic acid

PCR:

polymerase chain reaction

RAPD:

randomly amplified polymorphic DNA

RDA:

representational difference analysis

References

  • Antonetti, P.L.E., Pinon, J.: Somaclonal variation within popular.-Plant Cell Tissue Organ Cult. 35: 99–106, 1993.

    Article  Google Scholar 

  • Barrett, C., Lefort, F., Douglas, G.C.: Genetic characterization of oak seedlings, epicormic, crown and micropropagated shoots from mature trees by RAPD and microsatellite PCR.-Sci. Hort. 70: 319–330, 1997.

    Article  CAS  Google Scholar 

  • Bhatia, N.P., Bhatia, P., Ashwath, N.: Ex vitro rooting of micropropagated shoots of Stackhousia tryonii.-Biol. Plant. 45: 441–444, 2002

    Article  CAS  Google Scholar 

  • Bindiya, K., Kanwar, K.: Random amplified polymorphic DNA (RAPDs) markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L.-Euphytica 132: 41–47, 2003.

    Article  Google Scholar 

  • Butler, E.D., Gallagher, T.F.: Isolation and characterization of a cDNA encoding a novel 2-oxoacid-dependent dioxygenase which is up-regulated during adventitious root formation in apple (Malus domestica ‘Jork 9’) stem discs.-J. exp. Bot. 50: 551–552, 1999.

    Article  CAS  Google Scholar 

  • Butler, E.D., Gallagher, T.F.: Characterization of auxin-induced ARRO-1 expression in the primary root of Malus domestica.-J. exp. Bot. 51: 1765–1766, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Carvalho, L.C., Goulão, L., Oliveira, C., Gonçalves, J.C., Amâncio, S.: RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids.-Plant Cell Tissue Organ Cult. 77: 23–27, 2004.

    Article  CAS  Google Scholar 

  • De Klerk, G.J., Ter Brugge, J., Marinova, S.: Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’.-Plant Cell Tissue Organ Cult. 49: 39–44, 1997.

    Article  Google Scholar 

  • Epstein, E., Ludwig-Müller, J.: Indole-3-butyric acid in plants: occurrence, synthesis, metabolism and transport.-Physiol. Plant. 88: 382–389, 1993.

    Article  CAS  Google Scholar 

  • Feyissa, T., Welander, M., Negash, L.: Micropropagation of Hagenia abyssinica: a multipurpose tree.-Plant Cell Tissue Organ Cult. 80: 119–127, 2005a.

    Article  Google Scholar 

  • Feyissa, T., Welander, M., Negash, L.: In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants.-Plant Cell Rep. 24: 392–400, 2005b.

    Article  PubMed  CAS  Google Scholar 

  • George, E.F.: Plant Propagation by Tissue Culture: Part 2. In Practice. 2nd Edition.-xegetics Ltd., Edington 1996.

    Google Scholar 

  • Goto, S., Thakur, R.C., Ishii, K.: Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers.-Plant Cell Rep. 18: 193–197, 1998.

    Article  CAS  Google Scholar 

  • Hashmi, G., Huettel, R., Meyer, R., Krusberg, L., Hammerschlag, F.: RAPD analysis of somaclonal variants derived from embryo callus cultures of peach.-Plant Cell Rep. 16: 624–627, 1997.

    CAS  Google Scholar 

  • Hofmann, N.E., Raja, R., Nelson, R.L., Korban, S.S.: Mutagenesis of embryogenic cultures of soybean and detecting polymorphisms using RAPD markers.-Biol. Plant. 48: 173–177, 2004.

    Article  CAS  Google Scholar 

  • Isabel, N., Tremblay, L., Michaud, M., Tremblay, F.M., Bousquet, J.: RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.-Theor. appl. Genet. 86: 81–87, 1993.

    Article  CAS  Google Scholar 

  • Munthali, M.T., Newbury, H.J., Ford-Lloyd, B.V.: The detection of somaclonal variants of beet using RAPD.-Plant Cell Rep. 15: 474–478, 1996.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Negash, L.: Indigenous Trees of Ethiopia: Biology, Uses and Propagation Techniques.-SLU Reprocentralen, Umeå 1995.

    Google Scholar 

  • Potter, R., Jones, M.G.K.: An assessment of genetic stability of potato in vitro by molecular and phenotypic analysis.-Plant Sci. 76: 239–248, 1991.

    Article  CAS  Google Scholar 

  • Rahman, M.H., Rajora, O.P.: Microsatellite DNA somaclonal variation in micropropagated trembling aspen (Populus tremuloides).-Plant Cell Rep. 20: 531–536, 2001.

    Article  CAS  Google Scholar 

  • Rani, V., Parida, A., Raina, S.N.: Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh.-Plant Cell Rep. 14: 459–462, 1995.

    Article  CAS  Google Scholar 

  • Rout, G.R., Das, G.: An assessment of genetic integrity of micropropagated plants of Plumbago zeylanica by RAPD markers.-Biol. Plant. 45: 27–32, 2002.

    Article  CAS  Google Scholar 

  • Singh, A.K., Chand, S., Pattnaik, S., Chand, P.K.: Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo Roxb., a timber yielding tree legume.-Plant Cell Tissue Organ Cult. 68: 203–209, 2002.

    Article  Google Scholar 

  • Vallés, M.P., Wang, Z.Y., Montavon, P., Potrykus, I., Spangenberg, G.: Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue (Festuca pratensis Huds.).-Plant Cell Rep. 12: 101–106, 1993.

    Article  Google Scholar 

  • Wang, M., Oppedijk, B.J., Lu, X., Duijn, B.V., Schilperoort, R.A.: Apoptosis in barley aleurone during germination and its inhibition by abscisic acid.-Plant mol. Biol. 32: 1125–1134, 1996.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden

    T. Feyissa & M. Welander

  2. Department of Biology, Faculty of Science, Addis Ababa University, Addis Ababa, Ethiopia

    L. Negash

Authors
  1. T. Feyissa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Welander
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Negash
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Feyissa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feyissa, T., Welander, M. & Negash, L. Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica . Biol Plant 51, 15–21 (2007). https://doi.org/10.1007/s10535-007-0004-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-007-0004-1

Additional key words

Search

Navigation