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Evaluation of the genetic fidelity of in vitro propagated Ocimum basilicum L. using RAPD and ISSR markers

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Abstract

Genetic uniformity is one of the most important prerequisites for successful micropropagation of crop species. Genetic fidelity of in vitro raised 40 plants of Ocimum basilicum L. derived from nodal explants was assessed by 56 RAPD and 17 ISSR markers, for their genetic stability. Out of 56 RAPD and 17 ISSR primers screened, only 31 RAPD and 11 ISSR primers produced clear, reproducible, and scorable bands. The 31 RAPD primers produced 92 distinct and scorable loci, with an average of 2.96 loci per primer. The number of scorable loci for ISSR primers varied from three (ISSR-BG09 and ISSR-BG11) to eight (ISSR-BG01), with an average of 4.81 loci per primer. The number of loci generated per primer was greater in ISSR than RAPD. The markers designed from AG motif amplified more number of loci. The markers anchored at 3’ ends produced a higher number of consistent bands than unanchored markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. A similarity matrix based on Jaccard’s coefficient revealed that the pair-wise value between the mother and the in vitro-raised plantlets was 1, indicating 100% similarity. This confirmed the true-to-type nature of the in vitro-raised clones.

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Authors and Affiliations

  1. Cytogenetics & Plant Biotechnology Research Unit, University of Kalyani, Kalyani-741235, Nadia, West Bengal, India

    Soumen Saha & Parthadeb Ghosh

  2. Microbiology Research Laboratory, University of Kalyani, Kalyani-741235, Nadia, West Bengal, India

    Chandan Sengupta

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  1. Soumen Saha
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  2. Chandan Sengupta
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  3. Parthadeb Ghosh
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Correspondence to Parthadeb Ghosh.

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Saha, S., Sengupta, C. & Ghosh, P. Evaluation of the genetic fidelity of in vitro propagated Ocimum basilicum L. using RAPD and ISSR markers. J. Crop Sci. Biotechnol. 17, 281–287 (2014). https://doi.org/10.1007/s12892-014-0050-0

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  • DOI: https://doi.org/10.1007/s12892-014-0050-0

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