Abstract
The effective recovery of the immobilized enzymes using magnetic carriers has led to growing interest in this technology. The objective of this research was to evaluate the efficiency of immobilized laccase on magnetized multiwall carbon nanotubes (m-MWCNTs) in terms of stability and reusability. Laccases were efficiently adsorbed onto magnetized multiwall carbon nanotubes (m-MWCNTs) synthesized using water. The concentration of 7 mg laccase/mL was found to be ideal for immobilization. The optimal activity of both free and immobilized laccases was observed at pH 5, while for the latter, the optimal temperature was shifted from 40 to 50 °C. Compared to the free laccase, the immobilized laccase exhibited a greater range of stability at more extreme temperatures. At the fourth cycle of reactions, the immobilized laccase exhibited more than 60% relative activity in terms of reusability. Based on the fourier-transform infrared spectroscopy (FTIR) peak at 2921 cm−1, saccharification of paddy straw using immobilized laccase verified lignin degradation. The easy recovery of the immobilized laccase on m-MWCNTs lends credence to its potential use in biomass hydrolysis.
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Acknowledgements
We express our gratitude to Universiti Malaysia Perlis for granting us access to the laboratory to conduct the experiment. This work was supported by Fundamental Research Grant Scheme (FRGS), Grant No: 9003-00889.
Funding
Fundamental Research Grant Scheme (FRGS), Grant No: 9003-00889 [FRGS/1/2020/TK0/UNIMAP/03/13] entitled “Ligninolytic Kinetics and Thermodynamic Properties of Laccase from Lignin-degrading Microorganism for Delignification of Agricultural Waste from the Ministry of Higher Education Malaysia”.
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Yasmin, H.A.N., Kunasundari, B., Shuit, S.H. et al. Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes. Biotechnol Lett (2024). https://doi.org/10.1007/s10529-024-03494-z
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DOI: https://doi.org/10.1007/s10529-024-03494-z