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Lipase production by diverse phylogenetic clades of Aureobasidium pullulans

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Abstract

Thirty-nine strains representing 12 diverse phylogenetic clades of Aureobasidium pullulans were surveyed for lipase production using a quantitative assay. Strains in clades 4 and 10 produced 0.2–0.3 U lipase/ml, while color variant strain NRRL Y-2311-1 in clade 8 produced 0.54 U lipase/ml. Strains in clade 9, which exhibit a dark olivaceous pigment, produced the highest levels of lipase, with strain NRRL 62034 yielding 0.57 U lipase/ml. By comparison, Candida cylindracea strain NRRL Y-17506 produced 0.05 U lipase/ml under identical conditions. A. pullulans strain NRRL 62034 reached maximal lipase levels in 5 days on lipase induction medium, while A. pullulans strain NRRL Y-2311-1 and strains in clades 4 and 10 were highest after 6 days. A. pullulans strain NRRL Y-2311-1 and strains in clade 9 produced two extracellular proteins in common, at >50 and <37 kDa.

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Authors and Affiliations

  1. Renewable Product Technology Research Unit National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA

    Timothy D. Leathers, Joseph O. Rich & Amber M. Anderson

  2. Biochemistry Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, 52/347 Muang Ake, Phaholyothin Road, Lakhok, Pathumthani, 12000, Thailand

    Pennapa Manitchotpisit

Authors
  1. Timothy D. Leathers
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  2. Joseph O. Rich
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  3. Amber M. Anderson
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  4. Pennapa Manitchotpisit
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Correspondence to Timothy D. Leathers.

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Mention of any trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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Leathers, T.D., Rich, J.O., Anderson, A.M. et al. Lipase production by diverse phylogenetic clades of Aureobasidium pullulans . Biotechnol Lett 35, 1701–1706 (2013). https://doi.org/10.1007/s10529-013-1268-5

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  • DOI: https://doi.org/10.1007/s10529-013-1268-5

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