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Heavy oils produced by Aureobasidium pullulans

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Abstract

From a survey of more than 50 diverse strains of Aureobasidium pullulans, 21 produced extracellular heavy oils. Most oil producers fell into phylogenetic clades 8, 9, and 11. Oil colors ranged from bright yellow to malachite. More than half of the strains produced oil that was fluorescent. In medium containing 5% (w/v) sucrose, oil yields ranged from 0.5 to 6 g oil/l. Strain CU 43 reached stationary growth phase at day 4 while oil yields were maximal at day 6. CU 43 produced bright yellow, highly fluorescent oil that also was visible as intracellular droplets under fluorescent microscopy. Oil was surface active, suggesting that it functions as a biosurfactant. Oil from two strains (CU 43 and NRRL Y-12974) differentially inhibited mammalian cancer cell lines. MALDI-TOF MS spectra suggested that A. pullulans strains produce a family of related oil structures.

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Acknowledgments

The authors would like to acknowledge RSU grant number 37/52 from the Research Center of Rangsit University for financial support. The authors sincerely thank Dr. Christopher Dunlap for surface tension measurements. Appreciation is expressed for the kind assistance provided by Melinda Nunnally, Joseph Rich, Karen Hughes, Nathane Orwig, and Trina Hartman.

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Authors and Affiliations

  1. Biochemistry Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, 52/347 Muang Ake, Phaholyothin Rd., Lakhok, Pathumthani, 12000, Thailand

    Pennapa Manitchotpisit

  2. Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA

    Neil P. J. Price & Timothy D. Leathers

  3. Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Hunsa Punnapayak

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  1. Pennapa Manitchotpisit
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  2. Neil P. J. Price
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  3. Timothy D. Leathers
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  4. Hunsa Punnapayak
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Corresponding author

Correspondence to Timothy D. Leathers.

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Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

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Manitchotpisit, P., Price, N.P.J., Leathers, T.D. et al. Heavy oils produced by Aureobasidium pullulans . Biotechnol Lett 33, 1151–1157 (2011). https://doi.org/10.1007/s10529-011-0548-1

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  • DOI: https://doi.org/10.1007/s10529-011-0548-1

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