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Expression of cellulase genes in Saccharomyces cerevisiae via δ-integration subject to auxotrophic markers

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Abstract

δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase) were integrated into the S. cerevisiae W303-1A chromosome as reporters, by δ-integration with the aforementioned auxotrophic markers. Eight strains, L-BGL, H-BGL, T-BGL, U-BGL, L-EG, H-EG, T-EG, and U-EG, were selected and tested. After 5 days growth, cellulase activities (U ml−1) were 3, 2.3, 1.8, 1.5, 29, 12, 8, and 1.5, respectively. The results showed that auxotrophic markers influenced the expression of cellulase genes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30900033).

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Authors and Affiliations

  1. Tianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072, China

    Lin Liu, Cheng Liu, Shaolan Zou, Huajun Yang, Jiefang Hong, Yuanyuan Ma & Minhua Zhang

  2. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Lin Liu & Huajun Yang

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  1. Lin Liu
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  2. Cheng Liu
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  3. Shaolan Zou
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  4. Huajun Yang
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  5. Jiefang Hong
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  6. Yuanyuan Ma
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  7. Minhua Zhang
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Correspondence to Jiefang Hong.

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Liu, L., Liu, C., Zou, S. et al. Expression of cellulase genes in Saccharomyces cerevisiae via δ-integration subject to auxotrophic markers. Biotechnol Lett 35, 1303–1307 (2013). https://doi.org/10.1007/s10529-013-1205-7

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  • DOI: https://doi.org/10.1007/s10529-013-1205-7

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