Summary
Distribution of a delta (δ) sequence of the Ty element on a chromosome of the yeast Saccharomyces cerevisiae was analysed by pulsed-field gel electrophoresis. More than 100 copies of the δ sequence were non-randomly distributed on the chromosome. Using the δ sequence as a recombination site, mouse α-amylase and human β-endorphin genes were introduced into the chromosomal DNA. The integration occurred on a particular chromosome in each case and the copy number was estimated as three to five. It was also found that single- or multi-copy integration occured at a single or multiple sites on the particular chromosome. The integrants secreted α-amylase and β-endorphin by three-to fivefold compared with single-copy integrants. This type of integration was mitotically stable over a period of 50 generations under non-selective conditions.
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Sakai, A., Shimizu, Y. & Hishinuma, F. Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty. Appl Microbiol Biotechnol 33, 302–306 (1990). https://doi.org/10.1007/BF00164526
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DOI: https://doi.org/10.1007/BF00164526