Abstract
Saccharomyces pastorianus FBY0095 was mutated and variants were selected for efficient very high gravity brewing using 15% (w/v) maltose and 15% (w/v) ethanol. Two useful variants were obtained of which one (L6) had growth, wort consumption and ethanol production rates of 0.036, 1.13 and 0.49 g l−1 h−1, respectively. The corresponding results for the wild type were 0.028, 0.98 and 0.4 g l−1 h−1, respectively. The vitality of the variant (expressed as acidification power) was 2.5 while that of the wild type was 2.3. There was also an obvious improvement on flavor of resulting beer when using L6 and the other variant.
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Blieck L, Toye G, Dumortier F, Vertrepen KJ, Delvaux FD, Thevelein JM, Vandijck P (2007) Isolation and characterization of brewer’s yeast variants with improved fermentation performance under high-gravity conditions. Appl Environ Microbiol 73:815–824
Cooper DJ, Stewart GG, Bryce JH (1998) Some reasons why high gravity brewing has a negative effect on head retention. J Inst Brew 104:83–87
de Smidt O, du Preez JC, Albertyn J (2008) The alcohol dehydrogenases of Saccharomyces cerevisiae: a comprehensive review. FEMS Yeast Res 8:967–978
Gabriel P, Dienstbier M, Matoulková D, Kosař K, Sigler K (2008) Optimised acidification power test of yeast vitality and its use in brewing practice. J Inst Brew 114:270–276
Gibson B, Lawrence SJ, Leclaire JPR, Powell CD, Smart KA (2007) Yeast responses to stresses associated with industrial brewery handling. FEMS Microbiol Rev 31:535–569
Huuskonen A, Markkula T, Vidgren V, Lima L, Mulder L, Geurts W, Walsh M, Londesborough J (2010) Selection from industrial lager yeast strains of variants with improved fermentation performance in very-high-gravity worts. Appl Environ Microbiol 76:1563–1573
Kim SY, Lee BM, Cho JY (2010) Relationship between glucose catabolism and xanthan production in Xanthomonas oryzae pv. oryae. Biotechnol Lett 32:527–531
Liu L, Li Y, Shi Z, Du G, Chen J (2006) Enhancement of pyruvate productivity in Torulopsis glabrata: increase of NAD+ availability. J Biotechnol 126:173–185
Lodolo EJ, Kock JLF, Axcell BC, Brooks M (2008) The yeast Saccharomyces cerevisiae–the main character in beer brewing. FEMS Yeast Res 8:1018–1036
Pang Z, Liang J, Qin X, Wang J, Feng J, Huang R (2010) Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. Biotechnol Lett 32:1847–1851
Pereira FB, Guimarães PMR, Teixeira JA, Domingues L (2010) Selection of Saccharomyces cerevisiae strains for efficient very high gravity bio-ethanol fermentation processes. Biotechnol Lett 32:1655–1661
Pérez-Carrillo E, Cortés-Callejas LM, Sabillόn-Galeas LE, Montalvo-Villarreal JL, Canizo JR, Moreno-Zepeda MG, Serna-Saldivar SO (2011) Detrimental effect of increasing sugar concentrations on ethanol production from maize or decorticated sorghum mashes fermented with Saccharomyces cerevisiae or Zymomonas mobilis. Biotechnol Lett 33:301–307
Piddocke MP, Kreisz S, Heldt-Hansen HP, Nielsen KF, Olsson L (2009) Physiological characterization of brewer’s yeast in high-gravity beer fermentations with glucose or maltose syrups as adjuncts. Appl Microbiol Biot 84:453–464
Rautio JJ, Huuskonen A, Vuokko H, Vidgren V, Londesborough J (2007) Monitoring yeast physiology during very high gravity wort fermentations by frequent analysis of gene expression. Yeast 24:741–760
Vidgren V, Multanen JP, Ruohonen L, Londesborough J (2010) The temperature dependence of maltose transport in ale and lager strains of brewer’s yeast. FEMS Yeast Res 10:402–411
Viigand K, Alamäe T (2007) Further study of the Hansenula polymorpha MAL locus: characterization of the α-glucoside permease encoded by the HpMAL 2 gene. FEMS Yeast Res 7:1134–1144
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The authors gratefully acknowledge the National Natural Science Foundation of China (No. 31000810) and the Key Technology R&D Program of Guangdong Province (Nos. 2009A010700004, 2010A010500002 and 2011A020102001) for their financial supports.
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Yu, Z., Zhao, H., Li, H. et al. Selection of Saccharomyces pastorianus variants with improved fermentation performance under very high gravity wort conditions. Biotechnol Lett 34, 365–370 (2012). https://doi.org/10.1007/s10529-011-0780-8
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DOI: https://doi.org/10.1007/s10529-011-0780-8