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Screening and Mutation of Saccharomyces cerevisiae UV-20 with a High Yield of Second Generation Bioethanol and High Tolerance of Temperature, Glucose and Ethanol

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Abstract

A wild-type strain was isolated from slightly rotted pears after three rounds of enrichment culture, identified as Saccharomyces cerevisiae 3308, and evaluated for its fermentation capability of second generation bioethanol and tolerance of temperature, glucose and ethanol. S. cerevisiae 3308 was mutated by using the physical and chemical mutagenesis methods, ultraviolet (UV) and diethyl sulfate (DES), respectively. Positive mutated strains were mainly generated by the treatment of UV, but numerous negative mutations emerged under the treatment of DES. A positive mutated strain, UV-20, produced ethanol from 62.33 ± 1.34 to 122.22 ± 2.80 g/L at 30–45 °C, and had a maximum yield of ethanol at 37 °C. Furthermore, UV-20 produced 121.18 ± 2.51 g/L of second generation bioethanol at 37 °C. Simultaneously, UV-20 exhibited superior tolerance to 50% of glucose and 21% of ethanol. In a conclusion, all of these results indicated that UV-20 has a potential industrial application value.

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Acknowledgements

This work was funded by National Natural Science Foundation of China (21666010 and 31360217), and Doctoral Starting up Foundation of Jiangxi Normal University (5451).

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Authors and Affiliations

  1. College of Life Science, Jiangxi Normal University, Nanchang, 330022, China

    Shi Yi, Xiao Zhang, Han-xin Li, Xiao-xia Du, Shao-wei Liang & Xi-hua Zhao

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  1. Shi Yi
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  2. Xiao Zhang
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  3. Han-xin Li
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Correspondence to Xi-hua Zhao.

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Yi, S., Zhang, X., Li, Hx. et al. Screening and Mutation of Saccharomyces cerevisiae UV-20 with a High Yield of Second Generation Bioethanol and High Tolerance of Temperature, Glucose and Ethanol. Indian J Microbiol 58, 440–447 (2018). https://doi.org/10.1007/s12088-018-0741-1

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